Biogas production process with enzymatic pre-treatment

A technology of enzyme pretreatment and production method, which is applied in the direction of biofuel, waste fuel, fermentation, etc., can solve the problem of not being transformed, and achieve the effect of high productivity and low investment

Inactive Publication Date: 2013-01-16
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Processes for the production of biogas from biomass have not yet been optimized to achieve a theoretical total conversion to biogas, leaving fibrous lignocellulosic waste, which is not converted at all

Method used

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  • Biogas production process with enzymatic pre-treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0233] Example 1: Enzymatic liquefaction of biomass raw material for biogas production

[0234] The washing process is carried out under alkaline conditions, the purpose of which is to remove the soluble fraction of lignin and to swell the biomass material. Alkaline soluble compounds removed during washing include unwanted inhibitory substances to microorganisms and enzymes used in subsequent processing. During or after washing, cell wall degrading enzymes are used to enzymatically liquefy the biomass material and open up the recalcitrant structure of the biomass, which allows easier digestion of cellulose and other fermentable materials.

[0235] The main structural polysaccharides of lignocellulosic materials are generally composed of cellulose, hemicellulose (rich in neutral sugars), pectin materials containing D-galacturonic acid residues and mannan, which in different plant species It exists in combination with lignin in different proportions.

[0236] 1. Grind 200 g of...

Embodiment 2

[0260] Example 2: Enzymatic Digestion of Wheat Straw Biomass Material

[0261] 1. Grind 200 g of straw material for 2 minutes using a coffee grinder Bosch KM13 (E nr: MKM 6003FD 9512). A slurry of ground straw was prepared using 2000 mL of 1.2% NaOH with slow stirring at room temperature for 2 hours.

[0262] 2. Pour the material on a separating sieve with a mesh size of 0.295 mm, wash the material on the sieve with about 15 L of tap water and press with a spoon.

[0263] 3. Measure the pH of the filter cake to 8.1 using a WTW pH meter.

[0264] 4. A slurry 2000 g with 6.27% w / w dry matter was prepared and divided into 4 reactors, each containing 500 g, and the reactors were placed in a 50°C water bath.

[0265] 5. Table 1 above shows the activity of the enzyme products used in the test.

[0266] 6. The calculated enzyme dosage per gram of washed biomass shown in Table 6 was used for pretreatment.

[0267] 7. The hydrolysis reaction was carried out using the doses shown in...

Embodiment 3

[0282] Example 3: Enzymatic Digestion of Wheat Straw Biomass Material

[0283] 1. Grind 200 g of straw material for 2 minutes using a coffee grinder Bosch KM13 (E nr: MKM 6003FD 9512). A slurry of ground straw was prepared using 2000 mL of 1.2% NaOH with slow stirring at room temperature for 2 hours.

[0284] 2. Pour the material on a separation sieve with a mesh size of 0.295 mm, wash the material on the sieve with about 30 L of tap water and press with a spoon.

[0285] 3. Measure the pH of the filter cake to 7.8 using a WTW pH meter.

[0286]4. A slurry 2000 g with 6.26% w / w dry matter was prepared and divided into 4 reactors, each containing 500 g, and the reactors were placed in a 50°C water bath.

[0287] 5. Table 1 above shows the activity of the enzyme products used in the test.

[0288] 6. The calculated enzyme dosage per gram of washed biomass shown in Table 11 was used for hydrolysis pretreatment in each reactor.

[0289] 7. To verify by analysis that the enzyma...

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Abstract

A biogas production process with enzymatic pre-treatment, said process comprising the steps of providing a slurry comprising a lignocellulose-containing material, water and one or more enzyme; allowing the one or more enzyme to degrade the lignocellulose-containing material at a suitable temperature and pH; and adding the enzyme-degraded material to a biogas digester tank at a suitable rate and ratio to effectively convert the material to biogasin the digester.

Description

technical field [0001] The present invention relates to a method of biogas production with enzymatic pretreatment, said method comprising the steps of: providing a slurry comprising lignocellulose-containing material, water and one or more enzymes; at an appropriate temperature and pH, allowing the degrading the lignocellulose-containing material by the one or more enzymes; and adding the enzymatically degraded material to a biogas digester tank at an appropriate rate and ratio to efficiently convert the material in the digester for biogas. Background technique [0002] Most natural plant-based materials contain significant amounts of lignocellulosic fibers, which are either indigestible or only slowly digestible in many biological systems. As a result, for many biological processes that transform plant-based material, a significant portion of the treated material is not digested or is only poorly digested during processing. [0003] For example, in typical biogas producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12P7/08C12P7/10
CPCY02E50/16Y02E50/343C12P5/023C12P2201/00C12P7/10Y02E50/10Y02E50/30
Inventor H.S.奥尔森
Owner NOVOZYMES AS
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