Preparation method of plantain herb polyphenol with antioxidant activity

An antioxidant activity, plantain technology, applied in the fields of food and traditional Chinese medicine, can solve problems such as less research, and achieve the effects of large adsorption capacity, cost saving, and high physical and chemical stability

Inactive Publication Date: 2013-01-30
长春师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on plantain polyphenols

Method used

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  • Preparation method of plantain herb polyphenol with antioxidant activity
  • Preparation method of plantain herb polyphenol with antioxidant activity
  • Preparation method of plantain herb polyphenol with antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Extraction of plantain polyphenols

[0019] Add 100g of plantain coarse powder, add 800mL of 70% ethanol solution to reflux and extract 3 times, each time for 1 hour, filter, combine the filtrate, recover ethanol, add water to make 200mL extract, centrifuge at 5000rpm / min for 30min, discard the precipitate, The plantain polyphenol extract solution is obtained.

[0020] (2) Purification of plantain polyphenols

[0021] The plantain polyphenol extract was uploaded to the D101 macroporous resin column at a speed of 3BV / h (the sample volume was 20-30mL / g resin), after adsorption for 2 hours, it was first eluted with distilled water until the effluent was colorless, and then Use 30% ethanol as the elution solvent, elute at a rate of 4BV / h until colorless, collect the eluate, recover ethanol, and freeze-dry to obtain 3.8 g of plantain polyphenols.

Embodiment 2

[0023] (1) Extraction of plantain polyphenols

[0024] Add 1600 mL of 70% ethanol solution to 200g plantain coarse powder, reflux and extract 3 times, each time for 1 hour, filter, combine the filtrate, recover ethanol, add water to make 400mL extract, centrifuge at 5000rpm / min for 30min, discard the precipitate , to obtain the plantain polyphenol extract solution.

[0025] (2) Purification of plantain polyphenols

[0026] The plantain polyphenol extract was uploaded to the D101 macroporous resin column at a speed of 3BV / h (the sample volume was 20-30mL / g resin), after adsorption for 2 hours, it was first eluted with distilled water until the effluent was colorless, and then Use 30% ethanol as the elution solvent, elute at a rate of 4BV / h until colorless, collect the eluate, recover the ethanol, freeze-dry, and obtain 8 g of total polyphenols of Plantago.

Embodiment 3

[0028] (1) Extraction of plantain polyphenols

[0029] Add 2400 mL of 70% ethanol solution to 300g plantain coarse powder, reflux and extract 3 times, each time for 1 hour, filter, combine the filtrate, recover ethanol, add water to make 600mL extract, centrifuge at 5000rpm / min for 30min, discard the precipitate , to obtain the plantain polyphenol extract solution.

[0030] (2) Purification of plantain polyphenols

[0031] The plantain polyphenol extract was uploaded to the D101 macroporous resin column at a speed of 3BV / h (the sample volume was 20-30mL / g resin), after adsorption for 2 hours, it was first eluted with distilled water until the effluent was colorless, and then Use 30% ethanol as the elution solvent, elute at a rate of 4BV / h until colorless, collect the eluate, recover the ethanol, freeze-dry, and obtain 10 g of plantain polyphenols.

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Abstract

The invention provides a preparation method of plantain herb polyphenol with antioxidant activity, belonging to the technical field of the traditional chinese medicines and foods. The preparation method comprises the following steps of: a, smashing the plantain herb into coarse powder, carrying out reflux extraction by ethanol solution, wherein the reflux extraction time is 0.5-2 hours, recovering the ethanol after extraction, preparing the extraction solution by adding water, and abandoning precipitate to obtain plantain herb polyphenol extraction solution; b, charging the plantain herb polyphenol extraction solution onto a D101 macroporous resin column, wherein the absorption time is 1-3 hours, the elution solvent is a 10-90% ethanol solution, and the elution speed is 206BV / h; and carrying out ethanol elution to obtain the plantain herb polyphenol ethanol solution, recovering the ethanol, and carrying out freeze drying to obtain the plantain herb polyphenol. The preparation method of the plantain herb polyphenol has the beneficial effects that the macroporous resin is high in physical and chemical stability, high in adsorption capacity, good in selectivity, quick in adsorption speed, convenient in regenerating treatment, long in service life, cost-saving and the like, thereby being suitable for the industrial production. The prepared plantain herb polyphenol has the functions of radical removal and antioxidation. The preparation method has great developing potential in the aspects of the food, the drug, the cosmetics and the functional food.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine and food. Background technique [0002] Plant polyphenols are a class of complex compounds widely distributed in plants. Modern studies have shown that many diseases such as tissue and organ aging are related to excess free radicals in the body. Molecular damage plays a protective role. Therefore, polyphenolic compounds have good physiological activities in many aspects such as anti-aging, anti-tumor, anti-radiation, suppression of high blood pressure, and lowering blood fat. [0003] Plantain is the dry whole herb of Plantago asiatica L., a traditional Chinese medicine, and one of the plants that can be used as health food according to the Ministry of Health. , the effect of eliminating phlegm, and also has various pharmacological effects such as protecting the liver, lowering blood pressure, inhibiting bacteria, and lowering serum cholesterol. Great healthcare value. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/68A61K8/97A23L1/29A61P39/06A23L33/00
Inventor 刘春明王强王晶马冰
Owner 长春师范学院
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