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Composite manual bile duct and preparation method thereof

An artificial bile duct and tubular technology, applied in the field of composite artificial bile duct and its preparation, can solve problems such as biliary stricture, tissue adhesion, and death of experimental animals, and achieve the effects of low immunogenicity, excellent repair effect, and good clinical application prospects

Inactive Publication Date: 2014-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Gomez et al., "Comez NA, Zapafier JA, Vargas PE. Re: "Smallintestinal submucosa as a bioscafold for biliary tract regeneration", Surgery, 2004,135(4):460] questioned their research results, thinking that SIS is used for Repair of bile duct defects, which can cause tissue adhesions due to bile leakage, inflammation from a strong immune response, and other complex problems
According to the applicant's research, using bile duct acellular matrix alone to repair biliary defects will also cause bile leakage to cause tissue adhesions, biliary strictures, and inflammation caused by a strong immune response, eventually causing the death of experimental animals

Method used

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  • Composite manual bile duct and preparation method thereof
  • Composite manual bile duct and preparation method thereof
  • Composite manual bile duct and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of the composite artificial bile duct of the present invention

[0043] 1. Preparation of tubular acellular matrix:

[0044] Take an adult porcine bile duct, remove the fat and connective tissue around the lumen of the bile duct, and cut it into a 30mm long duct with a diameter of 4mm and a thickness of 1mm;

[0045] The pipeline was stirred and cleaned in PBS solution for 2 hours, soaked and stirred in 1% SDS solution for 24 hours;

[0046] Take out the PBS and wash for 2 hours, then soak and stir in 1% Triton-100 solution for 24 hours;

[0047] The decellularized scaffold was washed in PBS for 2 hours, fixed on a polytetrafluoroethylene rod with a diameter of 6 mm at the large end and a diameter of 4 mm at the small end, and freeze-dried to obtain a dry acellular matrix scaffold.

[0048]2. Coating the inner surface of the dried decellularized scaffold with a polyurethane aqueous emulsion with a concentration of 5 wt%, controlling the thickness...

Embodiment 2

[0049] Example 2 Preparation of the composite artificial bile duct of the present invention

[0050] 1. Preparation of tubular acellular matrix:

[0051] Take an adult pig bile duct, remove the fat and connective tissue around the lumen of the bile duct, and cut it into a 10mm long duct with a diameter of 6mm and a thickness of 2mm;

[0052] The pipeline was stirred and cleaned in PBS solution for 2 hours, soaked and stirred in 1% SDS solution for 24 hours;

[0053] Take out the PBS and wash for 2 hours, then soak and stir in 1% Triton-100 solution for 24 hours;

[0054] The decellularized scaffold was washed in PBS for 2 hours, fixed on a polytetrafluoroethylene rod with a diameter of 8 mm at the large end and a diameter of 6 mm at the small end, and freeze-dried to obtain a dry acellular matrix scaffold.

[0055] 2. Coat the inner surface of the dry decellularized scaffold with a polyurethane aqueous emulsion with a concentration of 15wt%, control the tube wall thickness t...

Embodiment 3

[0056] Example 3 Preparation of the composite artificial bile duct of the present invention

[0057] 1. Preparation of tubular acellular matrix:

[0058] Take an adult pig bile duct, remove the fat and connective tissue around the lumen of the bile duct, and cut it into a 10mm long duct with a diameter of 6mm and a thickness of 2mm;

[0059] The pipeline was stirred and cleaned in PBS solution for 2 hours, soaked and stirred in 1% SDS solution for 24 hours;

[0060] Take out the PBS and wash for 2 hours, then soak and stir in 1% Triton-100 solution for 24 hours;

[0061] The decellularized scaffold was washed in PBS for 2 hours, fixed on a cylindrical polytetrafluoroethylene rod with a diameter of 6 mm, and freeze-dried to obtain a dry decellularized matrix scaffold.

[0062] 2. Coat the inner surface of the dry decellularized scaffold with a polyurethane aqueous emulsion with a concentration of 15wt%, control the tube wall thickness to 1 mm, dry at 37°C for 24 hours, seal t...

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Abstract

The invention discloses a composite manual bile duct. A tubular acellular matrix made of a degradable high-molecular polymer material is coated on the inner surface and outer surface of the composite manual bile duct, wherein 2-20 mg of the degradable high-molecular polymer material is uniformly distributed on the inner surface of the acellular matrix for each square centimeter, and 0-20 mg of the degradable high-molecular polymer material is uniformly distributed on the outer surface of the acellular matrix for each square centimeter. The manual bile duct provided by the invention has the advantages of low immunogenicity, good in-vivo repairing effect and good clinical application prospect.

Description

technical field [0001] The invention relates to a composite artificial bile duct and a preparation method thereof. Background technique [0002] The extrahepatic bile duct is the only channel for bile to descend. Clinically, bile duct anastomosis is usually used in the treatment of diseases such as bile duct tumors, bile duct injury, bile duct congenital malformations and liver transplantation. This operation can dredge the biliary tract, but the operation changes the normal physiological pathway of the biliary tract, which will cause the loss of Oddi's sphincter function, and postoperative complications such as retrograde infection and anastomotic stenosis are prone to occur. Therefore, the development and research of artificial bile ducts that can be used in clinical treatment has very important practical significance. [0003] At present, there are many kinds of materials used to make artificial bile ducts. According to the degradability of materials, they can be divided...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/04A61L27/34A61L27/36
Inventor 程南生蒋霞张杰熊先泽林圯昕李富宇许瑞华陆燕蓉程惊秋
Owner WEST CHINA HOSPITAL SICHUAN UNIV