Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for preparing molecularly imprinted solid-phase extraction membrane in separation capillary

A separation capillary and solid-phase extraction membrane technology, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve the problems of difficult filling and fixing of polymer particles, large back pressure, dead volume, etc. problems, to achieve the effects of improved sensitivity, long service life, and reduced dosage

Inactive Publication Date: 2014-10-08
CHENGDU UNIVERSITY OF TECHNOLOGY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has problems such as dead volume caused by the interface, difficulty in filling and fixing polymer particles, and large back pressure.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing molecularly imprinted solid-phase extraction membrane in separation capillary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The polymerization solution was 0.05 mmol of methyltestosterone, 0.2 mmol of functional monomer methacrylic acid, 1 mmol of ethylene glycol dimethacrylate and 0.06 mmol of initiator azobisisobutyronitrile (AIBN) dissolved in 3 mL of acetonitrile Prepared.

[0024] The preparation method of the solid-phase extraction membrane imprinted on the column methyltestosterone is as follows:

[0025] (1) In the separation quartz capillary (100 μm in inner diameter, 60 cm in length), burn a window with a width of 3 mm near the injection port as the irradiation window of ultraviolet light; use 0.1 mol L -1 NaOH, 0.1 mol L -1 Rinse and activate the separation capillary with HCl for 30 min, then rinse with water and acetone for 5 min each, then dry the capillary with nitrogen flow;

[0026] (2) Suction 3-(methacryloyloxy)propyltrimethoxysilane acetone solution with a length of 1.5 cm and a volume ratio of 30% from the inlet port of the capillary through capillary action, seal both ...

Embodiment 2

[0030] The polymerization solution was 0.05 mmol of methyltestosterone, 0.2 mmol of functional monomer methacrylic acid, 1 mmol of ethylene glycol dimethacrylate and 0.06 mmol of initiator azobisisobutyronitrile (AIBN) dissolved in 3 mL of acetonitrile Prepared.

[0031] The preparation method of the solid-phase extraction membrane imprinted on the column methyltestosterone is as follows:

[0032] (1) In the separation quartz capillary (inner diameter 100 μm, length 60 cm), burn a window with a width of 3 mm near the injection port as the irradiation window for ultraviolet light; use 1 mol L -1 NaOH, 1 mol L -1 Rinse and activate the separation capillary with HCl for 30 min, then rinse with water and acetone for 5 min each, then dry the capillary with nitrogen flow;

[0033] (2) Suction 3-(methacryloyloxy)propyltrimethoxysilane acetone solution with a length of 1.5 cm and a volume ratio of 30% from the inlet port of the capillary through capillary action, seal both ends of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

A method for preparing a molecularly imprinted solid-phase extraction membrane in a separation capillary, mainly using ultraviolet light to initiate polymerization at the sampling end of the separation capillary to directly prepare a layer of molecularly imprinted polymer membrane with a length of several millimeters for use in capillary electrophoresis Online solid phase extraction. This method can be used to prepare various molecularly imprinted solid-phase extraction membranes in separation capillaries. The prepared capillary with molecularly imprinted solid-phase extraction membranes has the advantages of zero dead volume, avoiding polymer filling and fixing steps, and small back pressure. It can be used for online separation, enrichment and subsequent capillary electrophoresis analysis and determination of target substances in complex samples such as biology, medicine, environment and food.

Description

technical field [0001] The invention relates to a method for preparing a molecularly imprinted solid-phase extraction membrane in a separation capillary, and belongs to the technical field of sample pretreatment in capillary electrophoresis. Background technique [0002] Capillary electrophoresis technology has attracted extensive attention in recent years due to its advantages such as high resolution, fast analysis speed, and low sample consumption. Capillary electrophoresis has a variety of separation modes, which can be used for the analysis of inorganic ions, small organic molecules, peptides, proteins, nucleic acids and even cells. However, when applied to complex samples such as biology, medicine, environmental samples and food, this method needs to rely on efficient and highly selective sample pretreatment techniques. In capillary electrophoresis analysis, the sample pretreatment technology has two main purposes, namely, eliminating the matrix and enriching the targe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/281B01J20/28B01J20/30C08F222/14C08F220/06C08F4/04C08J9/26
Inventor 张信凤许淑霞王艳艳孙永华
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com