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Method for preparing ARA (Arachidonic Acid) by culturing mortierella alpina by utilizing solid material culture medium

A technology of arachidonic acid and Mortierella alpine, applied in the field of preparation of arachidonic acid, can solve the problems of low content, high development cost, instability, etc. Good synchronization effect

Active Publication Date: 2013-02-13
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past, commercial ARA was mainly derived from pork liver and fish oil, but the content was extremely low and unstable, and the development cost was expensive, which made it difficult to meet the needs of society, especially the large amount of ARA required by the dairy and pharmaceutical industries

Method used

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  • Method for preparing ARA (Arachidonic Acid) by culturing mortierella alpina by utilizing solid material culture medium
  • Method for preparing ARA (Arachidonic Acid) by culturing mortierella alpina by utilizing solid material culture medium
  • Method for preparing ARA (Arachidonic Acid) by culturing mortierella alpina by utilizing solid material culture medium

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Embodiment Construction

[0026] 1. Comparison of the small-scale fermentation results of liquid seeds and solid seeds

[0027] ① Liquid seed small-scale fermentation process: PDA slant seed → liquid shake flask cultivation → 30L seed tank (secondary seed) → 100L fermentation tank.

[0028] PDA slope production is familiar to people in this industry.

[0029] Liquid shake flask medium: glucose 30g / L, yeast powder 10g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.0g / L.

[0030] Secondary seed medium: glucose 30g / L, yeast powder 10g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.0g / L.

[0031] Fermentation medium: glucose 30g / L, yeast powder 15g / L, ammonium sulfate 0.5g / L, magnesium sulfate 0.5g / L, zinc sulfate 0.01g / L, potassium dihydrogen phosphate 1.0g / L, sodium glutamate 0.8 g / L.

[0032] Specific process: wash the PDA slant spores with 20mL sterile normal saline, insert them into a sterilized shaker flask containing 400mL seed medium, and cultivate them in a constan...

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Abstract

The invention discloses a method for preparing an ARA (Arachidonic Acid) by culturing a mortierella alpina by utilizing a solid material culture medium. The method comprises the following steps of using rice bran and niblet as material, adding inorganic salt in defined amount, using the inorganic salt as the solid material culture medium, collecting and smashing thallus after culturing strain in liquid, inoculating smashed thallus to the solid material culture medium, controlling culture temperature, mosturizing and controlling relative humidity during a culture process and appropriately stirring, and generating a large amount of spores. pH (Potential Of Hydrogen) segmentation regulation and control can be carried out during a fermentation process by using a citric acid and citrate. According to the method disclosed by the invention, the amount of seed spores of is large, the sunchronism is good, the activity is strong, the fermentation period of the mortierella alpina can be shortened, the growth of the mortierella alpine and the accumulation of ARA grease can be facilitated through the pH segmentation regulation and control, and the production and the quality of the ARA can be increased.

Description

technical field [0001] The invention relates to a method for preparing arachidonic acid in the technical field of microorganisms, in particular to the Mortierella alpina mycelium after liquid culture, which is crushed and inoculated into a solid medium, cultivated and propagated a large number of spores, and then prepared by liquid fermentation. Acrylic method. technical background [0002] Arachidonic acid (ARA) is an important precursor for the synthesis of prostaglandins in the human body. It has a series of physiological activities such as esterifying cholesterol, inhibiting platelet aggregation, increasing blood vessel elasticity, reducing blood viscosity, regulating white blood cell function, and improving immunity. In the past, commercial ARA was mainly derived from pig liver and fish oil, but the content was extremely low and unstable, and the development cost was expensive, which made it difficult to meet the needs of the society, especially the large amount of ARA ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 蔡金柱徐鲁明陈必钦朱友跑赖荣乾戴昌华吴轶詹光煌
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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