Method for purifying seaweed ketose

A technology of trehalulose and trehalulose ketose, which is applied in the field of bioengineering, can solve the problems of increasing the production cost of trehalulose, increasing the pressure of the production environment, and difficult crystallization of trehalulose, so as to reduce the pressure of the production environment and reduce the utilization rate , The effect of fast separation speed

Inactive Publication Date: 2013-02-13
GUANGXI UNIV
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Problems solved by technology

Sucrose and trehalulose are isomers, and trehalulose is difficult to crystallize and cannot be separated and purified by crystallization, so the separation of trehalulose is very difficult
[0004] At present, there is no relevant literature report on the separation and purification of trehalulose in China. The separation method of trehalulose and other sugar components can use gel resin or ion exchange resin to separate trehalulose from other

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  • Method for purifying seaweed ketose
  • Method for purifying seaweed ketose

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Embodiment Construction

[0024] Below by embodiment the present invention is described further, following experiment and example described content belong to the main content of the present invention, some belong to routine experimental method for those skilled in the art and do not appear in this patent description.

[0025] A method for purifying trehalulose, comprising the steps of:

[0026] 1. Culturing Saccharomyces cerevisiae cells

[0027] Inoculate the strain of Saccharomyces cerevisiae into the following medium (g / L): 20 grams of glucose, 20 grams of peptone, and 10 grams of yeast extract, then shake and culture overnight on a constant temperature shaker at 25-30°C until the OD600 is After 0.8, centrifuge at 5000 rpm for 3 minutes to collect yeast cells for the following assimilation step.

[0028] 2. Conversion of sucrose in sucrose solution into trehalose ketose by trehalose synthase

[0029] Add trehalose synthase derived from Thermobifida fusca reported in Chinese Patent No. ZL20041007...

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Abstract

The invention relates to a method for purifying seaweed ketose. The method comprises the following steps of: transforming 20% of sucrose solution by utilizing trehalose synthase to generate mixed syrup containing 80% of seaweed ketose; then assimilating sucrose which is not reacted in the mixed syrup, as well as byproducts, namely fructose and glucose by utilizing saccharomyces cerevisiae cells, wherein trehalose is very difficult to be degraded and assimilated by yeast; and further removing the saccharomyces cerevisiae cells in the syrup by utilizing centrifugation to get a seaweed ketose solution with single sugar component. According to the purification method, the recovery rate of the seaweed ketose can achieve 95%; and as for the seaweed ketose with the single sugar component, the syrup can be further concentrated, desalted and refined to produce the high-purity seaweed ketose.

Description

technical field [0001] The invention relates to the field of bioengineering, and belongs to the separation and purification of trehalulose syrup, in particular to a method for purifying trehalulose. Background technique [0002] Trehalulose (Trehalulose) and isomaltulose (1somaltulose) are both isomers of sucrose. Sucrose is a disaccharide formed by α, α-1, 2-glycosidic bonds. Seaweed Ketose is a disaccharide composed of a molecule of glucose and a molecule of fructose combined by α, α-1, 1-glycosidic bonds. Isomaltulose is a molecule of glucose and a molecule of fructose bonded by α, α-1 , a disaccharide formed by 6-glycosidic bonds. Most bacteria and yeast cannot ferment and utilize trehalulose and isomaltulose, so trehalulose and isomaltulose are good substitutes for sucrose in the fields of food and medicine. Although both trehalulose and isomaltulose are isomers of sucrose, their chemical and physical properties are very different. The sweetness of isomaltulose is 42%...

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Application Information

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IPC IPC(8): C12P19/12C12R1/865
Inventor 韦宇拓梁甲元杜丽琴黄日波
Owner GUANGXI UNIV
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