Unlock instant, AI-driven research and patent intelligence for your innovation.

Separation and purification method of GA4 (gibberellin A4) and/or GA7 (gibberellin A7)

A technology of separation and purification, separatory funnel, applied in the direction of organic chemistry, can solve the problems of difficult separation and purification, difficult to distinguish, etc., and achieve the effect of simple process, saving detection cost and large processing capacity

Active Publication Date: 2013-02-27
NANJING UNIV OF TECH
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production titer of strains after mutagenesis or improvement by means of genetic engineering has been improved, but a single GA still cannot be obtained. 4+7 , there are various gibberellin mixtures in the fermentation broth, which brings difficulties to the later separation and purification
GA 4 with GA 7 They are all 19 carbon gibberellins. There is only one double bond difference in their chemical structure, and their chemical properties are very similar, so it is difficult to distinguish them by general detection methods.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0021] Measure 600ml of Gibberella fermentation broth, after testing, GA 4+7 The mass accounted for 5% of the mass of Gibberella fermentation broth, adjusted the pH to 4.0 with HCl, added 200ml ethyl acetate, and oscillated and extracted at 120rpm for 0.5h to obtain the extract. Transfer the extract to a separatory funnel, let it stand until the layers are separated, and obtain the organic phase A and the solution respectively. Adjust the pH of the solution to 2.0 with HCl, then add 200ml of ethyl acetate, shake and extract at 120rpm for 0.5h to obtain an extract, transfer the extract to a separatory funnel, and let it stand until the layers are separated to obtain an organic phase B. Mix the obtained organic phase A and B to obtain 400ml of the organic phase mixture, add 1200ml of distilled water adjusted to pH 4.0 with HCl, shake and extract at 120rpm for 0.5h to obtain the extract, transfer the extract to a separatory funnel, and let stand After layering, 400 ml of organic...

Embodiment example 2

[0023] Measure 600ml gibberellin mixed solution, after testing, GA 4 The quality accounts for 70% of the quality of the gibberellin mixed solution, with H 2 SO 4 Adjust the pH to 5.5, add 150ml of pentanone, shake and extract at 140rpm for 1h to obtain the extract. Transfer the extract to a separatory funnel, let it stand until the layers are separated, and obtain the organic phase A and the solution respectively. The solution was washed with H 2 SO 4 Adjust the pH to 2.5, then add 150ml of pentanone, shake and extract at 140rpm for 1 hour to obtain an extract, transfer the extract to a separatory funnel, let stand until the layers are separated, and obtain an organic phase B. The obtained organic phases A and B were mixed to obtain 300ml of organic phase mixed solution, which was added with H 2 SO 4 Adjust 1200ml of distilled water with pH 5.0, vibrate and extract at 140rpm for 1h to obtain the extract, transfer the extract to a separatory funnel, let it stand until the...

Embodiment example 3

[0025] Measure 600ml of Gibberella mixed solution, after testing, GA 7 The mass of the mixture accounted for 90% of the mass of the Gibberella mixed solution, adjusted the pH to 7.0 with HCl, added 120ml of butanol, and oscillated and extracted at 160rpm for 1.5h to obtain the extract. Transfer the extract to a separatory funnel, let it stand until the layers are separated, and obtain the organic phase A and the solution respectively. Adjust the pH of the solution to 3.0 with dilute HCl, then add 120ml of butanol, shake and extract at 160rpm for 1.5h to obtain an extract, transfer the extract to a separatory funnel, and let it stand until the layers are separated to obtain an organic phase B. The obtained organic phase A and B are mixed to obtain 240 ml of organic phase mixed solution, which is added with H 2 SO 4 Adjust the pH of 1200ml of distilled water to 6.0, shake and extract at 160rpm for 1.5h to obtain the extract, transfer the extract to a separatory funnel, let it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a separation and purification method of GA4 (gibberellin A4) and / or GA7 (gibberellin A7), belonging to the field of biological separation. The separation and purification method of the GA4 and / or GA7 mainly comprises the steps of extraction, back extraction, depressurized concentration, separation through crystallization, melting point detection and high-performance liquid chromatographic detection. The method solves the problems that the GA4 and / or GA7 are difficult to separate and purify, a lack of high-efficiency detection techniques exists, requirements on equipment are high, the process is complex and the like in the prior art. By using the characteristic that the distribution coefficients of the GA4 and / or GA7 and other GAs (gibberellins) in different organic phases and a water phase are different, an extraction system is established, and after depressurized concentration and separation through crystallization, a quick melting point detection method is used for roughly judging the purity and then quantitative detection in combination with high-performance liquid chromatography. The separation and purification method of the GA4 and / or GA7 has the advantages that the separation process is simplified, the detection accuracy is improved, the cost is saved, the process is simple, the operation is easy to conduct and the method is suitable for industrial production.

Description

technical field [0001] The present invention relates to a GA 4 and / or GA 7 The separation and purification method of the invention relates specifically to extraction, back extraction, decompression concentration, and crystallization separation, belonging to the field of biological separation. Background technique [0002] Gibberellins (Gibbeerllins, referred to as GAs) is a natural plant growth regulator, which has a variety of physiological functions on plant growth, and can regulate the process of plant rooting, germination, growth, flowering and fruiting. Therefore, it is widely used in crop production and vegetable planting. Has a wide range of applications. More than one hundred species of gibberellins have been discovered, but they cannot be completely synthesized by chemical methods. The gibberellins produced industrially are all obtained by microbial fermentation. GA 3 It is the main product of bacterial fermentation and accumulation, and it is also the most phys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
Inventor 杨文革胡永红李佼佼唐容容
Owner NANJING UNIV OF TECH