Keratin-peptide-modified polyethylene glycol hydrogel, and preparation method and application thereof

A keratin peptide, polyethylene glycol technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problem of heavy environmental burden, high cost of synthetic peptides, and reproduction of natural biological materials. It has the effect of promoting cell adhesion and growth, and reducing production costs.

Inactive Publication Date: 2013-03-06
THE HONG KONG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a keratin peptide-modified polyethylene glycol hydrogel with biological functions in view of the

Method used

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  • Keratin-peptide-modified polyethylene glycol hydrogel, and preparation method and application thereof
  • Keratin-peptide-modified polyethylene glycol hydrogel, and preparation method and application thereof
  • Keratin-peptide-modified polyethylene glycol hydrogel, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Example 1 - Preparation of Vinyl Pegylated Keratin Peptides

[0060] Extraction of Keratin Peptides:

[0061] Get 60 grams of washed and dried wool and add it to 400ml of 4mol / L hydrochloric acid solution. React for 24 hours in a constant temperature water bath at 95°C. The obtained wool hydrolyzate is filtered, and sodium hydroxide solution is dropped in the obtained filtrate to adjust the keratin peptide solution to neutrality while stirring, and then spray-dried to form keratin peptide powder.

[0062] Preparation of vinyl PEGylated keratin peptides:

[0063]The keratin peptide powder was dissolved in 100 mM sodium bicarbonate solution until saturated, and the pH value of the resulting solution was adjusted to 8.2 by dropwise adding 1 M sodium hydroxide solution to form a keratin peptide solution. Filter through a filter with a pore size of 0.2 microns. Add 100 mg / ml of Acrl-PEG-NHS (MW3400) powder to the pH-adjusted keratin peptide solution, and react at room te...

Embodiment 2

[0064] Example 2 - Preparation of Vinyl Pegylated Keratin Peptides

[0065] Extraction of Keratin Peptides:

[0066] Get 50 grams of washed and dried human hair, and add it in 400ml hydrochloric acid solution of 3mol / L. React for 12 hours in a constant temperature water bath at 95°C. The obtained human hair hydrolyzate is filtered, and then freeze-dried into keratin peptide powder.

[0067] Preparation of vinyl PEGylated keratin peptides:

[0068] 2 g of keratin peptide powder was dissolved in 5 ml of 50 mM phosphate solution, and the pH value of the resulting solution was adjusted to 8.5 to form a keratin peptide solution. Filter through a filter with a pore size of 0.2 microns. After adding 50 mg / ml of Acrl-PEG-SCM (MW2000) powder to the pH-adjusted keratin peptide solution, it was reacted at 4° C. in the dark for 3 hours. The Acrl-PEG-Keratin solution obtained after the reaction was separated with a gel chromatographic column. The molecular weight of Acrl-PEG-Keratin ...

Embodiment 3

[0069] Example 3 - Preparation of Vinyl Pegylated Keratin Peptides

[0070] Extraction of Keratin Peptides:

[0071] Take 60 grams of washed and dried wool and add it to 400ml of 0.5mol / L sodium hydroxide solution. React in a constant temperature water bath at 100°C for 24 hours. The obtained wool hydrolyzate is filtered, hydrochloric acid solution is dropped in the obtained filtrate to adjust the keratin peptide solution to neutrality while stirring, and then spray-dried to form keratin peptide powder.

[0072] Preparation of vinyl PEGylated keratin peptides:

[0073] Dissolve keratin peptide powder in 100mM HEPES solution until saturated. The pH of the resulting solution was adjusted to 7.5 by dropwise addition of 1 M sodium hydroxide solution. Filter through a filter with a pore size of 0.2 microns. After adding 100 mg / ml of Acrl-PEG-SVA (MW5000) powder to the pH-adjusted keratin peptide solution, react at room temperature in the dark for 2 hours. The Acrl-PEG-Keratin...

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Abstract

The invention discloses a keratin-peptide-modified polyethylene glycol hydrogel, and a preparation method and an application thereof. The keratin-peptide-modified polyethylene glycol hydrogel comprises a copolymer of keratin-peptide-modified polyethylene glycol and polyethylene glycol acrylate monomer. According to the keratin-peptide-modified polyethylene glycol hydrogel provided by the invention, with the good biological performances of natural keratin peptide and the fine regulation capacity of polyethylene glycol hydrogel, the bio-functional material hydrogel product with promotion function upon cell adhesion and growth is obtained.

Description

technical field [0001] The invention relates to a biofunctional material, in particular to a keratin peptide-modified polyethylene glycol hydrogel, as well as its preparation method and application. Background technique [0002] The disadvantage of synthetic biomaterials that can be produced on a large scale with tunable structures and properties compared to natural biomaterials with poor reproducibility is poor biological performance. In order to develop and design new biomaterials, the modification of biologically inert synthetic materials with bioactive peptides is an important means to improve the bioactivity of synthetic materials. Appropriate bioactive peptide modification can enhance cell adhesion and promote cell proliferation, thereby improving the biological performance of synthetic materials. The most commonly used adhesion peptide RGD (amino acid sequence Arg-Gly-Asp) can be recognized by integrins on the cell membrane and participate in the adhesion between cel...

Claims

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Application Information

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IPC IPC(8): C08F299/02C08F2/48C08H1/00C08G65/48C12M3/00
Inventor 李翼胡军岩章瑜刘璇
Owner THE HONG KONG POLYTECHNIC UNIV
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