Method for preparing antioxidant peptide by utilizing ox hair

An antioxidant peptide, cattle hair technology, applied in the preparation methods of peptides, chemical instruments and methods, food preparation and other directions, can solve the problems of difficulty in recycling, short cattle hair fibers, waste disposal, etc., to achieve recycling, promote high value Transforming, easy-to-use effects

Active Publication Date: 2013-03-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the short fiber and dark color of cow hair, most of it is discarded as waste and difficult to recycle

Method used

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  • Method for preparing antioxidant peptide by utilizing ox hair
  • Method for preparing antioxidant peptide by utilizing ox hair
  • Method for preparing antioxidant peptide by utilizing ox hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Wash 50 parts of cow hair with water, drain / remove impurities, dry at 35°C for 8h, soak in 500 parts of NaOH solution with a concentration of 1wt% for 0.5h, wash with water until neutral, and use a hydrochloric acid solution with a concentration of 0.05M 1000 parts were soaked for 8 hours, then washed with water until neutral, drained, and dried for later use;

[0037] (2) put the cow hair after the above treatment into 500 parts of NaOH solution with a concentration of 0.5wt%, and under stirring, hydrolyze at a temperature of 40° C. for 48 hours to obtain a cow hair protein hydrolyzate;

[0038] (3) Centrifuge the above bovine hair protein hydrolyzate at 5,000rpm for 30min, take the supernatant, and dialyze in pure water for 48h with a dialysis bag with a molecular weight cut-off ≤ 10,000Da to obtain a bovine hair polypeptide solution;

[0039] (4) Concentrate the bovine hair peptide solution at a low temperature at a temperature of 35°C and a vacuum of 0.05MPa, an...

Embodiment 2

[0042] (1) Wash 50 parts of cow hair with water, drain / remove impurities, dry at 50°C for 6 hours, and use 2.5wt% NaCO 3 Soak 700 parts of the solution for 1 hour, wash with water until neutral, soak with 700 parts of sulfuric acid solution with a concentration of 0.3M for 5 hours, then wash with water until neutral, drain the water, and dry it for later use;

[0043] (2) put the cow hair after the above treatment into 700 parts of KOH solution with a concentration of 1.5wt%, and under stirring, hydrolyze at a temperature of 60° C. for 24 hours to obtain a cow hair protein hydrolyzate;

[0044] (3) Centrifuge the above bovine hair protein hydrolyzate at 8,000rpm for 20min, take the supernatant, and dialyze in pure water for 36h with a dialysis bag with a molecular weight cut-off ≤ 10,000Da to obtain a bovine hair polypeptide solution;

[0045] (4) Concentrate the bovine hair polypeptide solution at a low temperature and vacuum at a temperature of 40°C and a vacuum of 0.08MPa, ...

Embodiment 3

[0048] (1) Wash 50 parts of cow hair with water, drain / remove impurities, dry at 65°C for 4 hours, and use 5wt% NaHCO 3 Soak 1000 parts of the solution for 2 hours, wash with water until neutral, soak with 500 parts of nitric acid solution with a concentration of 0.5M for 2 hours, then wash with water until neutral, drain the water, and dry it for later use;

[0049] (2) Put the cow hair after the above treatment into Na with a concentration of 3wt% 2 CO 3 In 1000 parts of the solution, under agitation, the hydrolysis reaction was carried out at a temperature of 80° C. for 10 hours to obtain a oxalin hydrolyzate;

[0050] (3) Centrifuge the above-mentioned bovine hair protein hydrolyzate at 10,000rpm for 10min, take the supernatant, and dialyze in pure water for 12h with a dialysis bag with a molecular weight cut-off ≤ 10,000Da to obtain a bovine hair polypeptide solution;

[0051] (4) Concentrate the bovine hair polypeptide solution at a low temperature and vacuum at a temp...

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Abstract

The invention discloses a method for preparing antioxidant peptide by utilizing ox hair. The method is characterized by comprising the following steps of: cleaning 50 parts of ox hair with water, draining/removing impurities; drying for 4-8 hours at the temperature of 35-65 DEG C; soaking for 0.5-2 hours by using 500-1000 parts of 1-5wt% alkaline solution, and washing to be neutral; soaking for 2-8 hours by using 500-1000 parts of 0.05-0.5 M inorganic acid solution, then washing to be neutral, draining, airing, placing in 500-1000 parts of 0.5-3wt% alkaline solution, and conducting hydrolysis reaction for 10-48 hours at the temperature of 40-80 DEG C while stirring to obtain ox hair protein hydrolysate; centrifuging for 10-30 minutes under the condition of 5000-10000 rpm, and taking supernatant fluid, conducting dialysis for 12-48 hours in pure water by using a dialysis bag with molecular weight cutoff less than or equal to 10000 Da to obtain ox hair polypeptide solution; conducting low-temperature concentration under the conditions that the temperature is 35-45 DEG C and the vacuum degree is 0.05-0.1 MPa; and carrying out freeze-drying on concentrated solution under the conditions that the temperature is from-65 to -45 DEG C and the vacuum degree is 3-10 Pa for 12-48 hours to obtain the ox hair antioxidant peptide.

Description

technical field [0001] The invention relates to a method for preparing antioxidant peptides from cow hair, which belongs to the field of biological material preparation. Background technique [0002] Free radicals refer to atoms, atomic groups, ions or molecules containing unpaired electrons, which have high oxidative activity and are easy to react with biological macromolecules such as proteins, lipids and deoxyribonucleic acid (DNA), causing the molecules, cells and tissues of the body to Various injuries to organs accelerate the aging of the body and induce various chronic diseases. At the same time, free radicals can cause chain oxidation of food, seriously affecting the quality and safety of food. Scientific research shows that scavenging free radicals through antioxidant substances is an effective means to delay aging, prevent diseases, and inhibit food oxidation. At present, the widely used antioxidants are mainly chemically synthesized substances such as butylated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12A61K8/64A61K38/01A23L1/305
Inventor 曾维才石碧廖学品曹明蓉
Owner SICHUAN UNIV
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