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Preparation method of Fuzhuan tea golden flower fungus spore powder

A technology of golden flower fungus spore powder and Fu brick tea, which is applied in the direction of using spores, microorganism-based methods, biochemical equipment and methods, etc. and other problems, to achieve the effect of shortening the production cycle, reducing the pollution of miscellaneous bacteria and reducing the pollution of miscellaneous bacteria

Inactive Publication Date: 2013-03-13
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillus, Penicillium and other miscellaneous bacteria are easy to grow in naturally fermented Fuzhuan tea, which affects the normal growth of Jinhua fungus, thereby reducing the quality of Fuzhuan tea, and the fermentation period is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of Fuzhuan brick tea golden flower fungus spore powder, comprising the following steps:

[0028] Step 1) Bacteria activation: the golden flower fungus isolated and purified from Fuzhuan brick tea was inoculated into CZG (5% NaCl) slant medium (test tube 18×180mm), cultured at 28°C (aerobic), and the colonies formed after 5 days of cultivation When it turns golden yellow, the seed culture is obtained and set aside.

[0029] Step 2) Preparation of spore suspension: Add 30 mL of sterile water to the cultured seed culture above, wash off the spores of Chrysanthemum chrysogenum, and pour them into a conical flask with equal volume of glass beads by aseptic operation, at 100 r min -1 Shake for 10 minutes to obtain a uniformly dispersed spore suspension, which is set aside.

[0030] Step 3) Spore culture: take bran, add 80mL of water per 100g to prepare a solid medium, mix well and then pack into 250mL Erlenmeyer flasks, pave to a thickness of 1.0-1.3cm, ...

Embodiment 2

[0034] Step 1): Bacteria activation: the golden flower fungus isolated and purified from Fuzhuan tea was inoculated into CZG (5% NaCl) slant medium (test tube 15×150mm), cultured at 30°C, and the colonies were golden yellow when cultured for 5 days , that is, to obtain the seed culture, and set aside.

[0035] Step 2): Preparation of spore suspension: add 20 mL of sterile water to the above-mentioned cultivated seed culture, wash off the spores of Chrysanthemum spp. min -1 Shake for 10 minutes to obtain a uniformly dispersed spore suspension, which is set aside.

[0036] Step 3): Spore culture: take bran, add 100mL of water per 100g to prepare a solid medium, mix well and then pack into 500mL Erlenmeyer flasks, pave the thickness of 0.8 ~ 1.0cm, wrap it with eight layers of gauze, 121 Sterilize at ℃ for 30min. Immediately after the sterilization, shake the culture medium by shaking. Cool to room temperature in a sterile operating table, inoculate 2mL of the spore suspensio...

Embodiment 3

[0040] Step 1): Bacteria activation: the golden flower fungus isolated and purified from Fuzhuan tea was inoculated into CZG (5% NaCl) slant medium (test tube 20×200mm), cultured at 28°C, and the colonies were golden yellow when cultured for 5 days , that is, to obtain the seed culture, and set aside.

[0041]Step 2): Preparation of spore suspension: Add 30 mL of sterile water to the above-mentioned cultured seed culture, wash off the spores of Chrysanthemum chrysogenum, and pour them into a conical flask with equal volume of glass beads aseptically. min -1 Shake for 5 minutes to obtain a uniformly dispersed spore suspension, which is set aside.

[0042] Step 3): Spore culture: take bran, add 80mL of water per 100g to prepare a solid medium, mix well and then pack into 500mL Erlenmeyer flasks, pave the thickness of 0.8-1.0cm, wrap it with eight layers of gauze, 121 Sterilize at ℃ for 30min. Immediately after the sterilization, shake the culture medium by shaking. Cool to r...

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Abstract

The invention discloses a preparation method of Fuzhuan tea golden flower fungus spore powder, which comprising the steps of: inoculating golden flower fungus to a CZG slant culture medium, culturing for 5days at a temperature of 26-30 DEG C to obtain a seed culture; adding sterile water in the seed culture, washing down golden flower fungus spores, fully oscillating to obtain a uniformly-dispersed spore suspension; inoculating the spore suspension on the culture medium, carrying out constant-temperature culture at 26-30 DEG C, covering with a sterile breathable sealing film before white hypha germinates, removing the sterile breathable sealing film when yellow hypha germinates, continuously culturing for 3-5 days to obtain a yeast seed; then carrying out enlargement to obtain mouldy bran; and crushing the mouldy bran and then sieving to obtain the Fuzhuan tea golden flower fungus spore powder. The artificially cultured Fuzhuan tea golden flower fungus spore powder can be used for extracting effective components of the golden flower fungus and inoculating fermented Fuzhuan tea; and the fermentation period can be shortened and the pollution of infectious microbe is reduced.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation, and relates to a method for preparing Fuzhuan brick tea golden flower spore powder. Background technique [0002] Jinhua Fungus is a dominant bacterium with probiotic properties naturally formed during the flowering process of Fuzhuan Tea, and its scientific name is Eurotium cristatum. Fu brick tea is an ancient tea that is mainly sold in frontier areas. It has beneficial effects such as promoting body fluid to quench thirst, eliminating stagnation and losing weight. Research shows that this is inseparable from the existence of Jinhua fungus. Aspergillus, Penicillium and other miscellaneous bacteria are easy to grow in naturally fermented Fuzhuan tea, which affects the normal growth of Jinhua fungus, thereby reducing the quality of Fuzhuan tea, and the fermentation period is longer. If the spore powder of Jinhua fungus can be artificially cultivated and used to inoculate fermented Fuz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 吕嘉枥李玉婷
Owner SHAANXI UNIV OF SCI & TECH
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