Preparation method of high-purity diglyceride

A diglyceride, high-purity technology, applied in the field of preparation of high-purity diglyceride, can solve the problem of difficult separation of MAG, achieve the effect of simplifying the separation process, simplifying the separation process, and reducing energy consumption

Active Publication Date: 2013-03-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the defect that MAG is not easy to separate in the traditional high-purity di

Method used

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  • Preparation method of high-purity diglyceride

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Embodiment 1

[0029] Take 285g oleic acid (fatty acid composition: 80% oleic acid, 10% linoleic acid, 2% stearic acid, 8% palmitic acid) and 184g glycerin (molar ratio is 1:2) and 4.7g distilled water , put into a stoppered Erlenmeyer flask and mix evenly, and place it on a constant temperature magnetic stirrer with a rotating speed of 400rpm to react, add partial glyceride lipase LipaseSMG1 based on the total mass of the reaction mixture of 240U / g, and control the reaction temperature to 25°C; After 24 hours, the esterification product mixture was centrifuged, and the upper oil phase was recovered. The product composition is shown in Table 1. To the recovered oil phase, add water that is 4 times the molar number of monoglyceride (MAG), based on the total mass of the reaction mixture 200U / g of monoglyceride lipase MGL, put it into a stoppered Erlenmeyer flask, mix evenly, and place React on a magnetic stirrer with a rotation speed of 400rpm, program cooling (the initial reaction temperature...

Embodiment 2

[0031]Take 285g oleic acid (fatty acid composition: 80% oleic acid, 10% linoleic acid, 2% stearic acid, 8% palmitic acid) and 368g glycerin (molar ratio is about 1:4) and 6.5g Distilled water was put into a stoppered Erlenmeyer flask, mixed evenly, and placed on a constant temperature magnetic stirrer with a rotation speed of 400rpm for reaction, and partial glyceride lipase LipaseG50 based on the total mass of the reaction mixture of 240U / g was added, and the reaction temperature was controlled at 25°C; After reacting for 24 hours, the esterification product mixture was centrifuged, and the upper oil phase was recovered. The product composition is shown in Table 1. Add dehydrated ethanol 4 times relative to the MAG molar number to the recovered oil phase, based on the monoglyceride lipase bMGL of 200U / g of the total mass of the reaction mixture, put it into a stoppered Erlenmeyer flask and mix evenly, and place at a speed of The reaction was performed on a magnetic stirrer at...

Embodiment 3

[0033] Take 285g oleic acid (fatty acid composition: 80% oleic acid, 10% linoleic acid, 2% stearic acid, 8% palmitic acid) and 368g glycerin (molar ratio is about 1:4) and 6.5g Distilled water was put into a stoppered Erlenmeyer flask, mixed evenly, and placed on a constant temperature magnetic stirrer with a rotation speed of 400rpm for reaction, and partial glyceride lipase LipaseSMG1 based on the total mass of the reaction mixture of 240U / g was added, and the reaction temperature was controlled at 25°C; After reacting for 24 hours, the esterification product mixture was centrifuged, and the upper oil phase was recovered. The product composition is shown in Table 1. Add 6 times the water relative to the MAG mole number to the recovered oil phase, based on the monoglyceride lipase bMGL of 200U / g of the total mass of the reaction mixture, put it into a stoppered Erlenmeyer flask and mix it evenly, and place it in a rotating speed of 400rpm React on a magnetic stirrer, program ...

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Abstract

The invention discloses a preparation method of high-purity diglyceride, which comprises the following steps: 1) under the catalytic action of partial glyceride lipase, reacting a fatty acid donor and glycerol to synthesize a mixture containing diglyceride and monoglyceride, performing centrifugal separation on the mixture, and recovering the supernatant oil phase; 2) under the catalytic action of monoglyceride lipase, reacting the monoglyceride in the oil phase obtained in the step 1) and a hydroxyl donor, thus converting to obtain non-glyceride; 3) separating the non-glyceride component in the mixture obtained in the step 2) from the diglyceride, thus obtaining the high-purity diglyceride. According to the invention, the monoglyceride in the mixture is selectively removed through an enzyme method, the separation process is simplified, the energy consumption of the separation process is reduced, and the problem on the separation of MAG in the mixture is well solved. Meanwhile, the diglyceride content in the prepared product is up to 98% or above.

Description

technical field [0001] The invention relates to a preparation method of high-purity diglyceride. Background technique [0002] Diacylglycerol (DAG for short) is a product obtained by esterifying glycerol (glycerol) with two fatty acids. It is a natural component of oil and an intermediate product of oil metabolism. Diacylglycerol functional edible oil is a new type of oil modified by enzymatic or chemical methods from natural edible oil. It has clear functions of preventing obesity and lowering blood fat, and is safe to eat. It is a replacement product for ordinary edible oil. , can fundamentally alleviate the current situation of obesity, hyperlipidemia, diabetes and other chronic diseases caused by high-fat and high-calorie diet structure, and adjust the sub-health level of the human body and curb it without changing the existing eating habits. The occurrence and development of related chronic diseases. The relative content of DAG in natural oils mainly depends on the so...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 王永华杨博王卫飞蓝东明覃小丽
Owner SOUTH CHINA UNIV OF TECH
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