Separating and domesticating cultivation method for wild Yanqi black mushroom spawn

A technology for bacterial species isolation and cultivation methods, applied in the fields of botany equipment and methods, horticulture, application, etc.

Inactive Publication Date: 2013-03-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the domestication and cultivation of Yanqi black mushroom at present. The purpose of the present invention is to provide a method for the isolation and domestication of wild Yanqi black mushroom strains. Excellent new variety of fungus - Yanqi black mushroom, through the method of the present invention, so that wild Yanqi black mushroom can be domesticated and cultivated, and provide high-quality new varieties for the edible fungus market

Method used

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  • Separating and domesticating cultivation method for wild Yanqi black mushroom spawn
  • Separating and domesticating cultivation method for wild Yanqi black mushroom spawn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Strain isolation:

[0022] a. The conventional PDA medium is potato 200g / L, glucose 20g / L, agar powder 1%, and tissue separation method is used to inoculate 1 square millimeter bacterial block from the cap of wild Yanqi black mushroom and inoculate it in a test tube at a temperature of 25°C. 15 days to obtain white seed;

[0023] Liquid multiplication of parent species:

[0024] b. Use shake flasks to cultivate and propagate the original seed to obtain the mother seed. The liquid propagation medium is 100 g / L of wheat, 9 g / L of bran, 15 g / L of brown sugar, 2 g / L of yeast powder, and potassium dihydrogen phosphate 0.5 g / L, dipotassium hydrogen phosphate 0.5 g / L and magnesium sulfate 0.5 g / L, put the original seed in the test tube into the liquid medium at 25°C, and cultivate for 20 days to obtain the mother seed;

[0025] Cultivation medium formula and production:

[0026] c. Soak the green grass with 1% lime water, wash it with clear water, take it out and bottle it ...

Embodiment 2

[0031] Strain isolation:

[0032] a. The conventional PDA medium is potato 200g / L, glucose 20g / L, agar powder 1%, and tissue separation method is used to inoculate 1 square millimeter bacterial block from the cap of wild Yanqi black mushroom and inoculate it in a test tube at a temperature of 25°C. 10 days to obtain white seed;

[0033] Liquid multiplication of parent species:

[0034] b. Use shake flasks to cultivate and propagate the original seed to obtain the mother seed. The liquid propagation medium is 150 g / L of wheat, 12 g / L of bran, 20 g / L of brown sugar, 2.5 g / L of yeast powder, and potassium dihydrogen phosphate 1.5 g / L, dipotassium hydrogen phosphate 1 g / L and magnesium sulfate 1 g / L, put the original seed in the test tube into the liquid medium at 25°C, and cultivate for 15 days to obtain the mother seed;

[0035] Cultivation medium formula and production:

[0036] c. Soak the green grass with 3% lime water, wash it with clear water, pull it out and bottle it w...

Embodiment 3

[0041] Strain isolation:

[0042] a. Use conventional PDA medium as potato 200g / L, glucose 20g / L, agar powder 1%, and tissue separation method to inoculate about 1 square millimeter of bacteria block from the cap of wild Yanqi black mushroom, and inoculate it in a test tube at a temperature of 25°C Cultivate for 7 days to obtain white stock;

[0043] Liquid multiplication of parent species:

[0044] b. Use shake flasks to cultivate and propagate the original seed to obtain the mother seed. The liquid propagation medium is: wheat 200 g / L, bran 15 g / L, brown sugar 25 g / L, yeast powder 3 g / L, dihydrogen phosphate Potassium 2 g / L, dipotassium hydrogen phosphate 1.5 g / L and magnesium sulfate 1.5 g / L, the original seed of the test tube was inserted into the liquid medium, the temperature was 25°C, and the mother seed was obtained by culturing for 10 days;

[0045] Cultivation medium formula and production:

[0046] c, soak green grass with 5% lime water, clean with clear water, p...

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Abstract

The invention relates to s separating and artificial cultivation method for a wild Yanqi black mushroom spawn. The method comprises the following steps of: spawn separation, liquid propagation of stock culture, formulation and preparation of cultivating culture medium, and domesticating cultivation. By the adoption of the method, the product of Yanqi black mushroom with high quality can be obtained via artificial cultivation, and the excellent novel product of Yanqi black mushroom is provided for the market of edible fungi. The cultivation period of the Yanqi black mushroom obtained via the method is 4-5 months, a biological rate can reach above 50 percent, the pileus and the mushroom stalk of a fruiting body are white and are provided with tawny squamae, and after the pileus opens, the mushroom wrinkles are red brown. The obtained Yanqi black mushroom is provided with the high content of protein, delicious taste, and the unique quality of a wild mushroom. The mushroom has the functions of strong antioxidation and anti-aging, and is popular among people.

Description

technical field [0001] The invention belongs to the domestication and cultivation technology of edible fungus, and relates to strain separation, multiplication, cultivation material and domestication method of Yanqi black mushroom. Background technique [0002] Yanqi black mushroom is a unique high-quality wild edible fungus in Xinjiang. The fruiting bodies are mostly buried in sandy soil. The cap is large, the flesh is thick, the water content is low, the texture is crisp and tender, the fragrance is strong, the protein content is high, and it has strong anti-oxidation and anti-aging properties. Function. However, there is no report about the domestication and cultivation of black mushroom Yanqi at present. The purpose of the present invention is to provide a method for the isolation and domestication of wild black mushroom strains. The method can be eaten by separating, multiplying and domesticating The excellent new variety of fungus-Yanqi black mushroom can be artificia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 付振艳王晓军王建宝苟小清
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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