Initial culture medium special for preventing brown stain of strawberries and method for producing tissue culture strawberry seedlings by using initial culture medium

A culture medium and anti-browning technology, applied in the field of plant tissue culture, can solve browning and other problems, achieve the effects of preventing browning, reducing production and expansion, and enhancing activity

Inactive Publication Date: 2013-03-27
SUZHOU HEMEI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Initial culture medium special for preventing brown stain of strawberries and method for producing tissue culture strawberry seedlings by using initial culture medium

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Embodiment

[0029]In June-August, the selected plant grows vigorously, the stolon is substantial, and the tip grows well, and the excellent strawberry plant without damage by diseases and insect pests gets the explant of its shoot tip tissue culture. After trimming the shoot tip tissue, wrap it with gauze and rinse it under the tap for 2-4 hours, and then carry out surface disinfection. First rinse with 70% alcohol for half a minute, then soak with 0.1% bromogeramine for 15-20 minutes, then disinfect with 0.1% mercuric chloride (mercuric chloride) for 5-8 minutes, and finally disinfect with 1-2% peracetic acid 1 minute. Each time it should be rinsed with sterile water 3-4 times before the next disinfection. The entire disinfection process should be completed in an ultra-clean workbench.

[0030] Disinfect the inoculation equipment (tweezers, inoculation needles, inoculation racks, etc.) and let it cool naturally, then peel off the young leaves and scales outside the shoot tip layer by l...

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Abstract

The invention discloses an initial culture medium special for preventing brown stain of strawberries, which is prepared by taking an MS culture medium containing 0.2-0.5 mg/L of 6-benayl aminopurine (BA) and 0.1-0.2 mg/L of naphthylacetic acid (NAA) as a basic culture medium and adding vitamin C (VC) with the concentration of 5-15 mg/L and brassinolide (BR) with the concentration of 0.001-0.5 mg/L, wherein the brown stain rate in the initial strawberry tissue culture process can be greatly reduced; and the germination rate can be increased. The invention further discloses a method for producing tissue culture strawberry seedlings by culturing strawberry stem tip tissues through the culture medium, which comprises the steps of taking strawberry seedlings, sterilizing, inoculating, initially culturing, sub-culturing, acclimating, carrying out virus identification, breeding in a net yarn greenhouse and the like. According to the invention, the brown stain phenomenon in the initial strawberry culture can be effectively prevented; the anti-oxidant condition in the initially cultured strawberry tissues is effectively improved; and the germination rate is increased to be above 70% from 40-60%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium for preventing strawberry tissue culture from browning and a method for cultivating strawberry seedlings using the culture medium. technical background [0002] Strawberry is a perennial herb of Rosaceae Strawberry genus. It is one of the seven largest fruits in the world. It is rich in nutrition and has the reputation of "Queen of Fruits". In addition, eating strawberries has a certain curative effect on gastrointestinal diseases and anemia, and plays an important role in promoting intellectual development. It is deeply loved by consumers at home and abroad. [0003] Strawberry is an economic crop with short cultivation period, early results and quick results, but the virus damage of strawberry seriously affects the production of strawberry. Strawberry, as a high-grade berry, is mostly used for asexual propagation of seedlings in the current produ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 毕德
Owner SUZHOU HEMEI BIOTECH
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