Micro injection system for organic pollutant toxicity test and micro injection method and application thereof

A technology of organic pollutants and microinjection, applied in the direction of biochemical equipment and methods, specific-purpose bioreactors/fermenters, microbial determination/inspection, etc.

Inactive Publication Date: 2013-03-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to overcome the deficiency that it is difficult to realize accurate and fine non-injury injection in the process of organic pollutant toxicity test in the prior art, and provides a kind of microinjection system and microinjection method for organic pollutant toxicity t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of microinjection system that is used for the toxicity test of organic pollutant of the present embodiment, comprises microinjection host computer, injection arm, microinjection capillary, N 2 A steel cylinder, a vacuum pump, a stereo microscope, an agar plate and a stage micrometer, wherein: the microinjection capillary is drawn from a hollow borosilicate capillary glass tube, and the inner diameter of the microinjection capillary in this embodiment is 0.7 mm, and the outer diameter is 1mm, and breaks under a stereomicroscope, and the microinjection capillary is installed at the front end of the injection arm; N 2 The cylinder is connected to the microinjection host, N 2 Valve installed in N 2 At the outlet of the steel cylinder, the microinjection host controls the N 2 Valve regulation N 2 The air outlet pressure is used to adjust the droplet diameter at the tip of the microinjection capillary needle; the vacuum pump is used to suck the organic pollutant liq...

Embodiment 2

[0039] The microinjection system in this example is basically the same as in Example 1. In this example, zebrafish embryos are used as the injected body, and polybrominated diphenyl ether 2, 2', 4, 4'-tetrabromodiphenylether (BDE47) is injected, and the actual toxicity test is carried out. A kind of microinjection method that is used for organic pollutant toxicity test of the present embodiment, its concrete steps are:

[0040] (1) Preparation of organic pollutant solution: use DMSO to prepare a series of BDE-47 concentration gradients of 0, 0.1, 1, 10, 100 g / L, and store at -20 °C when not in use;

[0041] (2) Use a needle puller to pull the hollow borosilicate capillary glass tube into a microinjection capillary for injection, place the microinjection capillary on the stage of a stereo microscope, adjust the magnification, and see clearly in the field of view At the tip position, use the cover glass to lightly touch the microinjection capillary to make a small break. The inn...

Embodiment 3

[0049] The microinjection system of this example is basically the same as that of Example 1. In this example, zebrafish embryos are used as the injected body, 6-OH-BDE47 is injected, and the actual toxicity test is carried out. A kind of microinjection method that is used for organic pollutant toxicity test of the present embodiment, its concrete steps are:

[0050] (1) Preparation of organic pollutant solution: use DMSO to prepare a series of concentration gradients of 6-OH-BDE-47 at 0, 0.0156, 0.0625, 0.2500, 1.0000, 4.0000 g / L, and store at -20°C when not in use ;

[0051] (2) Use a needle puller to pull the hollow borosilicate capillary glass tube into a microinjection capillary for injection, place the microinjection capillary on the stage of a stereo microscope, adjust the magnification, and see clearly in the field of view At the tip position, use the cover glass to lightly touch the microinjection capillary to make a small break. The inner diameter of the microinjecti...

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Abstract

The invention discloses a micro injection system for an organic pollutant toxicity test and a micro injection method and the application thereof and belongs to the field of micro injection. The system comprises a micro injection main machine, an injection arm, a micro injection capillary, an N2 steel bottle, a vacuum pump, a stereoscopic microscope, an agar plate and a stage micrometer. The micro injection method includes (1) preparing organic pollutant solution; (2) preparing the micro injection capillary and the agar plate with the inner diameter of the micro injection capillary being 0.7mm and the outer diameter of the micro injection capillary being 1mm; (3) absorbing organic pollutant liquid to be injected into the micro injection capillary; (4) hanging the organic pollutant liquid at the needle point end of the micro injection capillary in hanging mode and controlling the volume of the injection liquid drop to reach a preset value; and (5) controlling the injection arm, injecting the organic pollutant liquid drop into a body to be injected. The micro injection system achieves accurate fine unharmful micro injection and provides technical support for evaluation of discharge, effectively control and management of pollutants.

Description

technical field [0001] The invention relates to the technical field of organic pollutant toxicity testing, more specifically, to a microinjection system, a microinjection method and an application for organic pollutant toxicity testing. Background technique [0002] At present, there are about 7 million kinds of known organic substances, of which hundreds of thousands are artificially synthesized, and the number is increasing at a rate of 2,000 every year. These organic substances enter the environment during various human social activities such as production, consumption, and transportation, and easily enter human and animal bodies through bioaccumulation and food chains. Some of these toxic and harmful pollutants can further accumulate in tissues such as liver, kidney, and heart. It causes many toxic reactions in the human body, including neurotoxicity, immunotoxicity, liver toxicity, endocrine disruption toxicity, reproductive and developmental toxicity, and carcinogenici...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/02
Inventor 刘红玲郑新梅陈怡于红霞
Owner NANJING UNIV
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