Bacillus mucilaginosus and preparation method of crude polysaccharide of bacillus mucilaginosus beneficial to growth of poultry

A technology of bacillus and crude polysaccharide, which is applied in the preparation method of Paenibacillus colloidum crude polysaccharide and the field of bacteria used in poultry growth, and achieves the effects of wide sources, low price, and reduced feed-to-meat ratio

Inactive Publication Date: 2013-03-27
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Utilization of capsular polysaccharide of this fun

Method used

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  • Bacillus mucilaginosus and preparation method of crude polysaccharide of bacillus mucilaginosus beneficial to growth of poultry

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Embodiment 1

[0038] Embodiment 1, the acquisition of Paenibacillus colloidus YC-13 bacterial strain:

[0039] Soil sample collection location: strawberry greenhouse in Yuancheng, Xiasha, Hangzhou.

[0040] Soil sample collection: adopt the S-shaped 5-point sampling mixing method for sampling, first remove the floating soil, collect the soil of 0~20cm in the plow layer, collect 500g of soil at each point, mix the soil at 5 points evenly, and take 500g of it by the five-point method As a soil sample, put it into a sterilization bag and store the soil sample at 4°C.

[0041] Separation and purification: Weigh 5g of soil sample into a sterile Erlenmeyer flask, add 45ml of sterile water, vibrate at 200rpm for 15min, and let it stand for 5min to obtain the stock solution of the bacterial suspension. Take 10 -1 、10 -2 and 10 -3 100 μl of bacterial suspension dilution (i.e. dilution 10 times, 100 times, 1000 times) was applied on the plate. Place the plate in an incubator at 30°C for 48 hours, a...

Embodiment 2

[0044] Embodiment 2, a kind of preparation method of the crude polysaccharide of Paenibacillus colloidus, selects Paenibacillus colloidus CCTCC 2012406 as the production bacterium; Carry out the following steps successively:

[0045] 1) Strain activation:

[0046] Paenibacillus colloidus CCTCC 2012406 was selectively cultured based on activation at 28°C for 24 hours, then a single colony was picked and inoculated in 100ml of seed fungus production medium, and cultured at 28°C with a shaker speed of 220r / min for 24 hours to obtain seeds bacteria.

[0047] The preparation method of selective medium is: in sucrose 8.0g, NaH 2 PO 4 0.8 g, MgSO 4 ·7H 2 O 0.5 g, CaCO 3 0.1 g, 17 g of agar powder, add water to make up to 1 L; then adjust the pH to 7.0 with 10 mol / L NaOH, and sterilize at 121°C for 20 minutes.

[0048] The preparation method of the seed fungus production medium is: in sucrose 10.0 g, K 2 HPO 4 0.5 g, MgSO 4 ·7H 2 O 0.5 g, (NH 4 ) SO 4 2.0g, CaCO 3 ...

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Abstract

The invention discloses a bacillus mucilaginosus with the preservation name of bacillus mucilaginosus YC-13# (Paenibacillus mucilaginosus YC-13#) and the preservation number of CCTCC No: M 2012406, which is preserved in the China typical culture collection center in the Wuhan University in Wuhan of China at October 16 in 2012. The invention also discloses an application of the bacillus mucilaginosus in the preparation of a feed additive beneficial to the growth of poultry and a preparation method of a crude polysaccharide of the bacillus mucilaginosus beneficial to the growth of poultry, wherein the preparation method comprises the steps of: activating a bacterial strain, cultivating by fermentation and extruding the crude polysaccharide.

Description

technical field [0001] The invention relates to a preparation method of Paenibacillus colloidum crude polysaccharide which is beneficial to the growth of poultry and the bacteria used. Background technique [0002] Microbial polysaccharides are biopolymers produced by microorganisms such as bacteria, fungi and cyanobacteria in the process of secondary metabolism that have protective effects on microorganisms. In recent years, more and more bacterial polysaccharides have played a wide range of roles in production and life. These bacterial polysaccharides can be produced using various waste residues and waste liquids under artificial control conditions, which reduces the pressure on environmental protection. The application prospect is better than that of animals and plants. Polysaccharides are more expansive. Research surveys in recent years by the biotechnology byproducts consortium (the biotechnology byproducts consortium, referred to as BBC, which is mainly committed to i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A23K1/16C12R1/07
Inventor 胡秀芳伍渡清钱鹏
Owner ZHEJIANG SCI-TECH UNIV
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