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Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation

A technology for edible fungi and nutritional powder, which is applied in the fields of botanical equipment and methods, food preparation, food forming, etc., can solve the problems of limited fruit body cultivation and utilization, and achieve the effect of promoting the efficiency of degradation, transformation and metabolism

Active Publication Date: 2013-04-03
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is not uncommon to use edible fungi to carry out research on the biotransformation and enrichment of inorganic selenium, but most of them are limited to the research on the cultivation and utilization of fruiting bodies; the research on the conversion of selenium-enriched liquid culture of edible fungi is also mainly found in academic reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The plate culture adopts PDA classic medium; the amount of wheat bran added for the optimized culture of the primary and secondary liquid strains is 1g / 100mL of wheat bran boiled filtrate and 3g / 100mL of crushed 20 mesh wheat bran powder, sodium selenite The dosage is 0.0μg / mL and 3.0μg / mL respectively, the dosage of yeast powder is 0.30g / 100mL, and the secondary strain fermentation tank is cultivated for 48 hours; the dosage of sodium selenite for the pretreatment of solid fermented wheat bran matrix is ​​3.0μg / g wheat bran, the water consumption for the preparation of sodium selenite solution is 90% of the amount of wheat bran used; the inoculum amount of bacteria for solid culture wheat bran is 30% of the wheat bran matrix, and it is placed at 25°C±0.5 and protected from light. , cultivated for 10 days; the selenium content of the selenium-enriched wheat bran powder prepared by solid fermentation is 0.90 mg / kg.

Embodiment 2

[0036] Plate culture adopts PDA enriched medium and 1g / 100mL wheat bran boiling filtrate; the wheat bran addition amount for the optimized culture of primary and secondary liquid strains is 1g / 100mL wheat bran boiling filtrate and 1.0g / 100mL respectively Wheat bran powder was crushed to 20 mesh, the dosage of sodium selenite was 1.0μg / mL and 3.0μg / mL respectively, the dosage of yeast powder was 0.15g / 100mL and 0.20g / 100mL respectively, and the secondary strain was cultured in shake flask for 96 hours The sodium selenite consumption of solid fermented wheat bran matrix pretreatment is 3.0 μ g / g wheat bran, and the water consumption of sodium selenite solution preparation is 90% of wheat bran consumption; 30% of the substrate was kept at 25°C ± 0.5°C, protected from light, and cultured for 10 days; air-dried at 55°C until constant weight. The product is the selenium-enriched wheat bran pear mushroom powder, and the selenium content of the selenium-enriched wheat bran powder prep...

Embodiment 3

[0038] Plate culture uses PDA enriched medium and 1g / 100mL wheat bran boiled filtrate; the addition of wheat bran for the optimized culture of primary and secondary liquid strains is 1g / 100mL wheat bran boiled filtrate and 3g / 100mL crushed wheat bran The dosage of sodium selenite was 1.0 μg / mL and 3.0 μg / mL respectively for wheat bran powder passing 20 mesh, and the dosage of yeast powder was 0.30 g / 100 mL; the dosage of sodium selenite for pretreatment of solid fermented wheat bran matrix was 3.0 μg / g wheat bran, the water consumption for the preparation of sodium selenite solution is 70% of the wheat bran consumption; the inoculum amount of the solid culture wheat bran is 30% of the wheat bran matrix, and it is placed at 25°C ± 0.5, protected from light , cultured for 12 days; air-dried at 55°C until constant weight. The product is the selenium-enriched wheat bran pear mushroom powder, and the selenium content of the selenium-enriched wheat bran powder prepared by solid ferm...

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PUM

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Abstract

The invention relates to a method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation. By adopting the method, an organic Se bran mushroom powder food raw material compounded by Se-enriched bran dietary fiber, edible mushroom vegetative hypha, extracellular bacteria polysaccharide and the like can be prepared. According to the method, pleurotus geesteranus Se-enriched liquid production bacteria are prepared by stimulation by adopting a liquid optimization cultivation technology for gradually optimizing bran and Se nutrition matrixes; the bran matrixes are subjected to high temperature and high pressure hydrolysis pretreatment by adopting a proper and safe dosage of sodium selenite; and finally, the bran is fermented, hydrolyzed and pretreated by Se-enriched pleurotus geesteranus liquid bacteria solids, so that the Se-enriched bran edible mushroom nutrition powder which contains organic Se raw materials and can be applied to Se-enriched food processing can be prepared.

Description

technical field [0001] The invention relates to a method for preparing selenium-enriched wheat bran edible fungus nutrient powder by solid culture. Background technique [0002] The trace element "selenium" is an important trace nutrient element in the physiological metabolism of organic organisms. In the last century, scientists have reported a large number of researches on its functional nutrition. Not only because of its importance, but also because the available resources of selenium in the natural distribution are extremely scarce, it has received great attention from natural science researchers in the world. According to reports by Chinese scientists in the 1980s, 70% of my country's arable land is located in the soil resource belt that lacks available selenium resources. Therefore, the development and utilization of biotransformation and enrichment technology to produce organic selenium nutrient resources has always been an important aspect of agriculture. An area o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23P1/06A23L1/28A01G1/04A23L33/00
Inventor 沈恒胜陈君琛汤葆莎吴俐张炎灼
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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