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Method for extracting and purifying sorrel smartweed flavonoids

A technology of Polygonum sorrel and flavonoids, which is applied in the field of extraction and purification of flavonoids from Polygonum sorrel, can solve the problems of few active ingredients, and no report on the extraction and purification process of Polygonum sorrel, flavonoids, etc., and achieve simple and easy operation line, reduce extraction time and reduce production cost

Inactive Publication Date: 2013-04-03
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polygonum sorrel is rich in flavonoids, but there are very few studies on the development and utilization of Polygonum sorrel resources and its active ingredients at home and abroad, and there is no report on the extraction and purification process of Polygonum sorrel flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Crush the air-dried or oven-dried Polygonum sorrel, pass through a 60-mesh sieve to obtain Polygonum sorrel powder, weigh 5g of Polygonum sorrel powder into a stoppered triangular bottle, add a certain amount of petroleum ether to soak, and the dosage Based on the submerged raw material, seal it, vibrate at 50°C and 70r / min for 3 hours, filter with a vacuum filter pump, remove the filtrate, dry and collect the grass powder to remove lipids and chlorophyll.

[0016] (2) Add 20 times the weight of the grass powder to the above-mentioned treated grass powder, and the concentration is 80% ethanol. Place the triangular flask in the ultrasonic extractor, raise the temperature to 70°C, then keep the temperature constant, turn on the ultrasonic wave, and use 60kHz ultrasonic wave for 30 minutes under the power of 400W, and stir during this period to make the grass powder evenly stressed; After the effect, filter with a vacuum pump to remove the grass powder, collect the fil...

Embodiment 2

[0022] (1) Crush the air-dried or oven-dried Polygonum sorrel, pass through a 60-mesh sieve to obtain Polygonum sorrel powder, weigh 5g of Polygonum sorrel powder into a stoppered triangular bottle, add a certain amount of petroleum ether to soak, and the dosage Based on the submerged raw material, seal it, vibrate at 50°C and 70r / min for 3 hours, filter with a vacuum filter pump, remove the filtrate, dry and collect the grass powder to remove lipids and chlorophyll.

[0023] (2) Add 30 times the weight of the grass powder to the above-mentioned treated grass powder, and the concentration is 65% ethanol. Place the triangular flask in the ultrasonic extractor, raise the temperature to 60°C, then keep the temperature constant, turn on the ultrasonic wave, and use 60kHz ultrasonic wave for 35 minutes under 400W power, and stir during this period to make the grass powder evenly stressed; After the effect, filter with a vacuum pump to remove the grass powder, collect the filtrate, ...

Embodiment 3

[0029] (1) Crush the air-dried or oven-dried Polygonum sorrel, pass through a 60-mesh sieve to obtain Polygonum sorrel powder, weigh 5g of Polygonum sorrel powder into a stoppered triangular bottle, add a certain amount of petroleum ether to soak, and the dosage Based on the submerged raw material, seal it, vibrate at 50°C and 70r / min for 3 hours, filter with a vacuum filter pump, remove the filtrate, dry and collect the grass powder to remove lipids and chlorophyll.

[0030] (2) Add 40 times the weight of the grass powder to the above-mentioned treated grass powder, and the concentration is 70% ethanol. Place the triangular flask in the ultrasonic extractor, raise the temperature to 70°C, then keep the temperature constant, turn on the ultrasonic wave, and use 60kHz ultrasonic wave for 45 minutes under the power of 400W, and stir during this period to make the grass powder evenly stressed; After the effect, filter with a vacuum pump to remove the grass powder, collect the fil...

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PUM

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Abstract

The invention relates to a method for extracting and purifying sorrel smartweed flavonoids, which is capable of being produced in large scale, simple in process, low in cost and high in efficiency. The method comprises the following steps: sorrel smartweed grass meal is added with petroleum ether to be soaked and vibrated for 2 to 6 hours at the temperature of 40 to 70 DEG C and 50 to 110 r / min, added with 50 to 80% of ethanol of which the ratio of solid to liquid is 1: 10 to 1: 40 after filtration and drying, heated for 15 to 60 min under the action of ultrasonic at the temperature of 40 to 70 DEG C and filtered, and then the filtrate is concentrated to 1 / 3 volume, and freeze-drying is carried out to obtain the flavonoids crude product of which the purity is 37%; flavonoids are purified by the macroporous resin chromatography: the flavonoids crude product is dissolved in water and prepared to a solution of which the concentration is 3 to 12 mg / mL, the solution is loaded to a column under the conditions that the sample volume is 25 mL, the mobility is 0.5 to 2.5 mL / min, and the sample solution is pH 5 to 7, then the macroporous resin is rinsed by the distilled water until the flushing fluid is colorless and eluted by 60 to 90% of ethanol at the mobility of 0.5 to 2.5 mL / min until the eluate is colorless, the eluate is collected and concentrated to 1 / 3 volume, and freeze-drying is carried out to obtain the pure crystal of flavonoids of which the purity is more than 70%.

Description

technical field [0001] The invention belongs to a method for extracting and purifying plant active ingredients, in particular to a method for extracting and purifying flavonoids from Polygonum sorrel leaves. Background technique [0002] Bioflavonoids (bioflavonoids) is a kind of phenolic substances that widely exist in nature, also known as vitamin P, often accompanied by vitamin C, is an important class of natural organic compounds produced by plant photosynthesis. Flavonoids are one of the main active ingredients in natural medicinal plants, which have physiological activities such as regulating blood lipids, eliminating oxygen free radicals, anti-oxidation, anti-tumor, and anti-virus. At present, there are reports about extracting flavonoids from ginkgo leaves, hawthorn leaves, mulberry leaves, roses and other materials. Flavonoids have broad application prospects in the fields of health care products, medicine, food and feed additives, but there are still raw material s...

Claims

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Application Information

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IPC IPC(8): A61K36/70A23K1/16
Inventor 朱宇旌张勇李艳
Owner SHENYANG AGRI UNIV
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