Method for continuously producing L-ornithine composite salt by enzyme immobilization

An enzyme immobilization, ornithine technology, applied in the direction of immobilization on/in organic carriers, fermentation, etc., can solve the complex strain control and production control, restrict the promotion and popularization of production methods, and unstable product quality, etc. problems, to achieve the effects of easy molding, high production yield and convenient handling

Active Publication Date: 2013-04-03
WUHAN SHENGJIA CHEM ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the Japanese Meizhisu company that has industrialized the production of ornithine by fermentation, there are also problems such as low acid production rate of strains, difficulty in later separation, unstable production due to differences in strain passage, and relatively complicated strain control and production control and many other factors, the actual production cost is much higher than that of chemical hydrolysis and enzymatic hydrolysis, and the product quality is not stable. The selection and cultivation technology of strains and high equipment investment have seriously restricted the promotion of this production method. and popularity

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  • Method for continuously producing L-ornithine composite salt by enzyme immobilization

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Experimental program
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Effect test

Embodiment 1

[0023] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, filter, wash the filtered resin with pure water, and then mix according to the mass ratio of resin: sodium carbonate: pure water = 1:0.3:30 Form a solution, adjust the pH value of the solution to 9, stir and lower the temperature to ≤-5°C, slowly add sodium carbonate with a mass of 0.3% of the resin dropwise, and after holding for 2 hours, add sodium carbonate with a concentration of 1% and a mass of 0.001% of the mass of the resin Enzyme aqueous solution, warmed up to 0°C for 10 hours, filtered out the resin, washed three times with 10 times pure water to obtain immobilized enzyme resin;

[0024] ②Put 1000L of deionized water into a 2000L reactor, add 300kg of L-arginine while stirring, and raise the temperature to 25°C to obtain an aqueous solution of L-arginine with a mass concentration of 300g / l;

[0025] ③Put 150g of manganese acetate into the reaction kettle, stir for 10 minute...

Embodiment 2

[0028] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, filter, wash the filtered resin with pure water, and then mix according to the mass ratio of resin: sodium carbonate: pure water = 1:0.5:60 To form a solution, adjust the pH value of the solution to 11, stir and lower the temperature to ≤-5°C, slowly add cyanogen bromide with a mass of 0.5% of the resin, and keep it for 2 hours, then add a concentration of 1% and a mass of 0.003% of the resin The enzyme aqueous solution was heated to 5°C for 13 hours, the resin was filtered out, and washed three times with 10 times pure water to obtain the immobilized enzyme resin;

[0029] ②Put 1000L of deionized water into a 2000L reactor, add 400kg of L-arginine while stirring, and raise the temperature to 40°C to obtain an aqueous solution of L-arginine with a mass concentration of 400g / l;

[0030] ③ Add 200g of manganese acetate into the reaction kettle, stir for 10 minutes, add 8 kg of immobili...

Embodiment 3

[0033] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, then filter, wash the filtered resin with pure water, and then mix it according to the mass ratio of resin: sodium carbonate: pure water = 1:0.8:100 Form a solution, adjust the pH value of the solution to 13, stir and lower the temperature to ≤-5°C, slowly add cyanogen bromide with a mass of 0.8% of the resin, and keep it for 2 hours, then add a concentration of 1% and a mass of 0.005% of the mass of the resin The enzyme aqueous solution was heated to 10°C for 16 hours, the resin was filtered out, and washed three times with 10 times pure water to obtain the immobilized enzyme resin;

[0034] ②Put 1000L of deionized water into a 2000L reactor, add 500kg of L-arginine while stirring, and raise the temperature to 55°C to obtain an aqueous solution of L-arginine with a mass concentration of 500g / l;

[0035] ③Put 250g of manganese acetate into the reaction kettle, stir for 10 minutes, ad...

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Abstract

The invention discloses a method for continuously producing an L-ornithine composite salt by enzyme immobilization. The method comprises the following steps of: selecting a macroporous adsorption resin with amidogen matrix, carrying out a special treatment by using a curing agent during a combined immobilization process of an enzyme and the resin, so that amidogens of inner surfaces and outer surfaces of holes of the macroporous adsorption resin are reacted to form a firm covalent structure during an adequate contact process of an arginine hydrolytic enzyme and the macroporous adsorption resin, and removing surface purities through simple eluting so as to reach an immobilization purpose. According to the method, the advantages that an enzymic method is high in production yield, good in quality and stable in quality are kept, the disadvantages that enzyme cannot be recycled during an enzymic method production process, so that the use cost is high and continuous production cannot be performed are solved; and meanwhile the L-ornithine composite salt can be synthesized in one step, and the method is high in yield, low in energy consumption, simple in process and stable in quality.

Description

technical field [0001] The invention relates to a method for preparing amino acid, in particular to a method for continuously producing L-ornithine compound salt with enzyme immobilization. Background technique [0002] L-Ornithine is an important non-protein basic amino acid with important activity and physiological functions. It has both nutritional and therapeutic functions and is widely used in medical care, chemical industry and other fields. [0003] At present, the synthesis of L-ornithine mainly includes chemical synthesis, chemical hydrolysis, biological enzyme hydrolysis and bacterial fermentation. The chemical synthesis method mainly uses acrolein, hydrogen cyanide, ammonia, carbon dioxide, etc. as raw materials for chemical synthesis. The main disadvantage of this method is that there are many steps and the yield is not high. At the same time, the product obtained is active L-ornithine The mixture with inactive D-ornithine is difficult to separate in the later s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/10C12N11/08
Inventor 印常智
Owner WUHAN SHENGJIA CHEM ENG
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