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Method for judging ending point of sweet corn blanching with content of hexanal

A discrimination method, sweet corn technology, applied in the field of judging the end point of sweet corn blanching, can solve the problems of publication, lack of test data, and lack of establishment of the corresponding relationship between lipoxygenase activity and blanching end point, so as to improve accuracy Effect

Active Publication Date: 2015-01-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as far as sweet corn is concerned, although some foreign experts have proposed the inactivation of lipoxygenase as a sign of the degree of blanching of sweet corn, there has been no relevant experimental data published, and no relationship between lipoxygenase activity and blanching end point has been established. Correspondence

Method used

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  • Method for judging ending point of sweet corn blanching with content of hexanal
  • Method for judging ending point of sweet corn blanching with content of hexanal
  • Method for judging ending point of sweet corn blanching with content of hexanal

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Experimental program
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Effect test

Embodiment

[0026] 1. Experimental instruments and reagents

[0027] 1) Experimental equipment

[0028] Agilent 7890 GC-8963 MS coupled instrument (Agilent, USA)

[0029] Heating magnetic stirrer (PC-420, Beijing Kanglin Technology Co., Ltd.)

[0030] CAR / PDMS / DVB extraction head (Supelco, USA)

[0031] 2) Experimental reagents

[0032] Hexanal standard (≥98%, Sigma, USA)

[0033] Chromatographically pure methanol (American Tiandi Company)

[0034] C 6 -C 20 Series of n-paraffin standard products (Sigma, USA)

[0035] 2. Experimental design

[0036] In this example, the method of homogenizing corn and buffer solution is used to fully contact and react the enzyme with the substrate;

[0037] Using the purchased hexanal reference sample, combined with the calculation of the retention index and the Agilent 7890GC-8963MS coupled instrument, the detected hexanal was qualitatively analyzed;

[0038] Establish a standard curve for the determination of hexanal for quantitative analysis;...

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Abstract

The invention provides a method for judging ending point of sweet corn blanching, comprising the following concrete steps: 1) after liquid nitrogen quick freezing of sweet corn, carrying out ice-bath homogenization with phosphate buffer with a pH of 7.0 and a proportion of 1: 4 (w / v); 2) taking 8mL homogenate in an extraction bottle, warming up to 60 DEG C, and carrying out headspace solid-phase microextraction for 20 min at the temperature of 60 DEG C; 3) detecting volatile ingredients with gas chromatograph-mass spectrometer method, resolving for 2 min at the temperature of 220 DEG C; 4) integrating a mass chromatogram, retention time alignment and retention index of a standard sample so as to carry out qualitative analysis on hexanal under the conditions of setting a temperature program and separating a chromatographic column strip, and then carrying out qualitative analysis according to a specification curve; and 5) when the content of hexanal in the sweet corn is lower than 4 microgram per gram, determining the ending point of blanching. The test results show that the method has the advantages of being high in rate of accuracy, good in correlation with quality of quick-freeze sweet corn in storage period, and the like.

Description

1. Technical field [0001] The invention relates to a method for measuring the amount of hexanal produced in sweet corn, which can be used for judging the blanching end point of sweet corn. 2. Background technology [0002] Sweet corn refers to the corn that is harvested and used for food during the milk-maturing stage. Sweet corn has higher nutrients than old corn in terms of amino acids, water-soluble proteins, vitamins and trace mineral elements, and sweet corn has good palatability, sweet taste, and is easy to eat. The characteristics of digestion and absorption are welcomed by consumers. [0003] However, because sweet corn is in the vigorous period of corn life activities when harvested, the quality drops rapidly after harvest, and quick freezing is the most commonly used processing method for sweet corn. However, at low temperatures, enzymes still maintain a certain activity, especially some enzymes involved in fat oxidation make quick-frozen corn prone to oxidative, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 牛丽影刘夫国李大婧刘春泉刘春菊
Owner JIANGSU ACAD OF AGRI SCI
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