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Composition for preventing or treating osteoporosis, containing ssangwhatang or lactobacillus fermented product thereof

A kind of technology of osteoporosis, soup lactic acid bacteria, applied in the field of compositions for prevention and/or treatment

Active Publication Date: 2013-04-10
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is almost no relevant literature on Shuanghetang fermented medicinal materials.

Method used

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  • Composition for preventing or treating osteoporosis, containing ssangwhatang or lactobacillus fermented product thereof
  • Composition for preventing or treating osteoporosis, containing ssangwhatang or lactobacillus fermented product thereof
  • Composition for preventing or treating osteoporosis, containing ssangwhatang or lactobacillus fermented product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: make the lactic acid bacteria fermented product of Shuanghe Tang

[0041] The Shuanghe Decoction is a Chinese medicine preparation made from Radix Paeoniae Alba, Rehmannia glutinosa, Radix Astragali, Angelica, Rhizoma Chuanxiong, Cinnamon Bark, Licorice, Ginger, and Jujube. One pair was used as a unit to make 50 pairs for this experiment. The dosage for one batch is 9.37g of white peony root, 3.75g of Rehmannia glutinosa, 3.75g of astragalus, 3.75g of angelica, 3.75g of Chuanxiong, 2.81g of cinnamon, 2.81g of licorice, 1.49g of ginger, and 2.0g of jujube. extract. The total amount of 50 pairs of medicinal materials is 1,674g, add 10 times the amount of distilled water 16.74L, heat for 150 minutes to extract (Korea, Kyungseo medicine machine cosmos-600), using a standard testing sieve (Standard testing sieve, Aperture 500 μm and 150 μm) to make Shuanghe soup. Add 1% volume (v / v) of lactic acid bacteria (1-5 × 10 8 CFU / ml), after fermenting at 37°C for...

Embodiment 2

[0042] Example 2: Effects of Shuanghe Decoction and Fermented Shuanghe Decoction on Osteoclast Differentiation

[0043] RAW264.7 cells exhibited an increase in TRAP activity as a marker of osteoclast differentiation upon RANKL treatment and differentiated into osteoclasts (Minkin C. Calcif Tissue Int. 1982 34(3): 285-90; Hsu H, et al. al., 1999, Proc. Nat. Acad. Sci. USA, 96, 35403545). In order to confirm the influence of Shuanghetang (SHT) and Shuanghetang lactic acid bacteria fermented product (SFT) made in Example 1 on osteoclast differentiation, RAW264.7 cells were divided into 1×10 3 Density of cells / well, seeded in 96-well plate, treated with Shuanghetang and Shuanghetang fermented product at a concentration of 25-200μg / ml for 3 days, using α-MEM medium containing differentiation-inducing factor RANKL (100ng / ml) (10% FBS) as medium.

[0044] On day 4 after the experimental treatment, TRAP activity was detected after performing TRAP staining to confirm the formation ...

Embodiment 3

[0046] Example 3. Expression of osteoclast differentiation-related genes in Shuanghe Decoction

[0047] It is known that TRAP, c-Src and cathepsin K genes are essential genes related to osteoclast differentiation and bone resorption function, and their expression increases during osteoclast differentiation (Hsu H, et al., 1999, Proc.Nat.Acad . Sci. USA, 96, 35403545). In order to confirm the effect of Shuanghetang, which inhibits TRAP staining and activity, on the expression of the three genes, real time quantitative PCR (real time quantitative PCR) was performed. For this, RANKL treatment was performed and after 24 hours, total RNA was isolated using TRIzol (Invitrogen, Inc. NY, USA). Specific primers for genes related to osteoclast differentiation were designed by an online primer design program (Rozen and Skaletsky, 2000), and the primers for each gene were used as forward (Forward) and reverse (Reverse) primers shown in Table 1 below right. cDNA synthesis using 2 μg o...

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Abstract

The present invention relates to a composition for preventing or treating osteoporosis, and more specifically, to a pharmaceutical composition and a dietary supplement for preventing or treating osteoporosis, containing Ssangwhatang, or a lactobacillus fermented product thereof prepared by inoculating a lactobacillus to Ssangwhatang and fermenting the same, as an active ingredient. The composition containing Ssangwhatang or lactobacillus fermented product thereof of the present invention can remarkably suppress the differentiation of osteoclast and the expression of related genes and increases bone density in in vivo experiment, and thus can be used for preventing and / or treating osteoporosis.

Description

technical field [0001] The present invention relates to a composition for preventing and treating osteoporosis, in particular to a composition containing Shuanghe decoction or its lactic acid bacteria fermented product as an active ingredient, which can be used for preventing and / or treating osteoporosis. Background technique [0002] The body's bone tissue has been in a repeated dynamic balance between osteoblast formation tissue and osteoclast resorption tissue. When osteoblasts and osteoclasts are out of balance, bone resorption exceeds bone formation, leading to osteoporosis, which is characterized by a decrease in calcium in bone tissue, thinning of bone compact, and enlargement of the bone marrow cavity; such a state Continued, bone hardness will decrease, easy to cause fractures. There are many known causes of osteoporosis, such as increasing age, lack of exercise, low body weight, smoking, decreased calcium intake, amenorrhea, post-ovariectomy surgery, and the like....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/65A61K36/804A61K36/232A61P19/10
CPCA61K36/72A61P19/10
Inventor 马珍烈沈棋奭李智慧
Owner KOREA INST OF ORIENTAL MEDICINE
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