Pesticide composition with functions of preventing and controlling nematodes
A technology of pesticide composition and nematodes, which is applied in the direction of nematicides, biocides, animal repellents, etc., can solve the problems of mixing thiazophosphine and marigold extracts and no reports on the application, so as to reduce the cost of medication, Obvious synergistic effect and effect of improving drug efficacy
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[0021] The preparation method of above-mentioned prochloraz (prochloraz manganese salt) comprises the following steps:
[0022] Thiazophosphine 6.0%; Marigold Extract 4.0%; Attapulgite 2.0%; Polyvinylpyrrolidone 0.15%; Reactive Blue 0.1%; Quartz Sand 87.75%.
[0023] According to the preparation method of medicinal fertilizer granules of the present invention, the above-mentioned materials are mixed evenly in a closed preparation kettle, granulated by coating method, dried at 120°C until the water content of the granules is 1%, and sieved, and the particle size range is 4000 microns (μm) ), after passing the test, 10% thiazophosphine marigold extract granules were obtained.
Embodiment 1
[0024] Bioassay Example 1: Indoor bioassay test on cucumber root-knot nematode by mixing thiazophosphine and marigold extract.
[0025] Test method: Take the cucumber root system that produces a large number of root knots, rinse it gently with tap water, gently pick out the milky white oocysts on the diseased root with a dissecting needle, put it into a small petri dish with a diameter of 6 cm, add a small amount of sterile water, and dry it at 25 Incubate in an incubator at ℃ for 3-4 days, collect the 2nd instar larvae, and add sterile water to prepare a suspension with a certain concentration (about 500 / ml) for later use.
[0026] Five concentration gradient treatments were set, and sterile water was used as the blank control. Take a 96-well cell culture plate, add 150 microliters of different concentrations of prepared medicines to each well, then add an equal volume of nematode suspension, and culture at 25°C with moisture, and check the 2nd instar of A. incognita at diffe...
Embodiment 2
[0032] Biological Assay Example 2: Field efficacy test of mixture of thiazophosphine and marigold extract against cucumber root-knot nematode.
[0033] Test drugs: (1) 10% thiazophosphine · marigold extract granules, (2) 10% thiazophosphine granules, (3) 10% marigold extract granules.
[0034] Application time: water the roots 7 days after the cucumbers are transplanted.
[0035] Test results: see Table 2, as can be seen from the test results: 20 days and 30 days after application, the amount of 10% thiazophosphine marigold extract granule preparation of the present invention is 1000-2000 g / mu to control cucumber The effect of root-knot nematode is more than 85.92%, which is obviously better than that of a single dose, indicating that the pesticide composition of the present invention can well prevent and treat crop nematodes, reduce dosage, reduce costs, delay the generation of nematode resistance, and reduce environmental pollution.
[0036] Table 2. Field efficacy test res...
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