Method for preparing molecular imprinting porous gel membrane for tissue engineering

A technology of molecular imprinting and tissue engineering, applied in the field of functional materials and biomaterials, can solve the problems of lack of identification of tissue engineering materials, uncontrollable thickness of hydrogel film, etc.

Inactive Publication Date: 2013-04-17
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Aiming at the deficiencies of the prior art, the technical problems to be solved by the present invention are uncontrollable hydrogel film thickness, lack of identification and selective release ability of tissue engineering materials, etc.
[0011] The technical solution of the present invention to solve the problems of the uncontrollable thickness of the hydrogel film and the lack of identification and selective slow-release ability of tissue engineering materials is to design a molecularly imprinted porous gel film for tissue engineering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. A preparation method of epidermal growth factor molecularly imprinted porous gel membrane for tissue engineering

[0024]A) preparation mass percentage is 1% sodium alginate, mass percentage is 0.5% carboxymethyl chitosan, mass percentage is 0.01% epidermal growth factor and mass percentage is the mixed aqueous solution of 0.5% polyethylene glycol, Standing for use after defoaming; prepare an aqueous solution of calcium chloride with a mass percentage concentration of 15% at the same time;

[0025] b) 1 g of the sodium alginate mixed aqueous solution containing carboxymethyl chitosan, epidermal growth factor and polyethylene glycol obtained in step a) is poured into a flat glass sheet with a dry surface, and a scraper with a thickness of 20 μm is used. The film rod is scraped flat to form a film of uniform thickness, and then immediately immersed in the calcium chloride aqueous solution prepared in step a) together with the glass sheet, continued to soak for ...

Embodiment 2

[0028] Example 2. A method for preparing a fibroblast growth factor molecularly imprinted porous gel membrane for tissue engineering

[0029] a) preparation mass percentage is 5% sodium alginate, mass percentage is 0.1% polyacrylic acid, mass percentage is 0.01% fibroblast growth factor and mass percentage is the mixed aqueous solution of 0.5% polyvinyl alcohol, static disinfection Stand-by after soaking; prepare a calcium chloride aqueous solution with a mass percentage concentration of 0.5% at the same time;

[0030] b) Take 5 g of the sodium alginate mixed aqueous solution containing polyacrylic acid, fibroblast growth factor and polyvinyl alcohol obtained in step a), pour it into a flat glass sheet with a clean surface, and scrape it with a scraper stick with a thickness of 1000 μm flat, forming a film of uniform thickness, then immediately soaked in the calcium chloride aqueous solution prepared in step a) together with the glass sheet, continued to soak for 12 hours, and...

Embodiment 3

[0033] Example 3. A method for preparing a vancomycin molecularly imprinted porous gel membrane for tissue engineering

[0034] a) preparation mass percentage is 2% sodium alginate, mass percentage is 1% polymethacrylic acid, mass percentage is the mixed aqueous solution of 0.05% vancomycin and 2% polyvinylpyrrolidone, let stand Stand-by after defoaming; prepare a calcium chloride aqueous solution with a mass percentage concentration of 2% at the same time;

[0035] b) Take 2 g of the sodium alginate mixed aqueous solution containing polymethacrylic acid, vancomycin and polyvinylpyrrolidone obtained in step a), pour it into a flat glass sheet with a clean surface, and use a squeegee stick with a thickness of 200 μm Scrape off to form a film of uniform thickness, then immediately soak into the calcium chloride aqueous solution prepared in step a) together with the glass sheet, continue to soak for 12 hours, rinse 3-5 times with deionized water, and obtain the vancomycin-embedde...

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Abstract

The invention provides a method for preparing a molecular imprinting porous gel membrane for tissue engineering. Bioactive molecules such as growth factor, polypeptide, protein and the like are taken as template molecules, natural polymer sodium alginate which has good biocompatibility and can be absorbed in a degradable manner is used as a basal body, water-soluble polymer or inorganic salt serves as a pore-forming agent, and a green environment-friendly aqueous-phase imprinting method is adopted to prepare the molecular imprinting porous gel membrane with the thickness controlled between 20 and 1000mu m. The method is simple to operate and does not use any toxic and harmful solvents; the adopted materials have good biocompatibility; and cell culture porous gel membrane stents with identification and slow release performances to different template molecules can be obtained according to cell culture requirements and are used for regulating cell growth, and the method has a good application prospect in the tissue engineering field.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted porous gel membrane used for tissue engineering, and belongs to the field of functional materials and biological materials. Background technique [0002] Tissue engineering applies the principles and methods of cell biology and engineering, starting from understanding the relationship between normal and pathological tissue structure and function, researching and developing biological substitutes to restore, maintain or improve the shape and function of damaged tissues and organs, and achieve Restoration and reconstruction purposes. One of the key technologies in tissue engineering is to prepare biocompatible, degradable and absorbable cell scaffolds. Hydrogels are polymers that swell in water and hold large amounts of water without dissolving, much like body tissues that are filled with large amounts of aqueous fluid. The soft, wet surface and affinity with tissue greatly reduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/52A61L27/56
Inventor 赵孔银林贝贝阚伯红范文佳王霞
Owner TIANJIN POLYTECHNIC UNIV
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