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Bacterial strain with ACE (Angiotensin Converting Enzyme) inhibitory activity for leavening agent of meat product

A technology for inhibiting activity, meat products, applied in the direction of microbial-based methods, bacteria, microorganisms, etc.

Active Publication Date: 2013-04-17
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to lactic acid bacteria, there is no research on other microorganisms producing ACE inhibitory activity at home and abroad

Method used

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  • Bacterial strain with ACE (Angiotensin Converting Enzyme) inhibitory activity for leavening agent of meat product
  • Bacterial strain with ACE (Angiotensin Converting Enzyme) inhibitory activity for leavening agent of meat product
  • Bacterial strain with ACE (Angiotensin Converting Enzyme) inhibitory activity for leavening agent of meat product

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specific Embodiment approach 1

[0021] In this embodiment, the bacterial strain with ACE inhibitory activity used in meat product starter is isolated and purified from fermented beans produced by traditional methods, and its sample is currently stored in the Microbiological Laboratory of Heilongjiang Bayi Agricultural Reclamation University; it is carried out according to the following steps Separation and purification culture:

[0022] 1. Culture medium:

[0023] Nutrient agar (g / L): 10.0g peptone, 3.0g beef powder, 5.0g sodium chloride, 15.0g agar, 1000mL distilled water, pH 7.3±0.1, autoclaved at 121°C for 15 minutes.

[0024] Nutrient broth (g / L): peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, distilled water 1000mL, pH value 7.2±0.2, autoclave at 121°C for 15 minutes.

[0025] Casein medium II: 1g casein, 1g yeast extract, 8g sodium chloride, 4g agar powder, 200mL distilled water, pH 7.2~7.4.

[0026] Lipase activity detection medium II: 15% lard, 0.2g neutral red indicator, 100mL nutrient aga...

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Abstract

The invention provides a bacterial strain with ACE (Angiotensin Converting Enzyme) inhibitory activity for a leaving agent of a meat product, and relates to bacillus licheniformis. The bacterial strain with the ACE (Angiotensin Converting Enzyme) inhibitory activity for the leaving agent of the meat product is bacillus licheniformis (Bacillus licheniformis) F2 and is stored in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.6574 on September 18, 2012. The bacterial strain F2 has the ACE inhibition ratio of 83.13%, can bear 6% of NaCl and 100mg / kg of nitrite, and cannot generate acid; the bacterial strain is remarkable in proteinase activity, and is obvious in the effect of inhibiting staphylococcus aureus; the bacterial strain serving as the bacterial strain of the leaving agent can be coordinated with other bacterial strains in proper condition to produce fermented foods or to independently produce foods or fungicide with the ACE inhibitory activity.

Description

technical field [0001] The invention relates to a strain of bacillus licheniformis. Background technique [0002] Hypertension is a worldwide problem, affecting 15-20% of adults. Among the various factors that control blood pressure in the human body, renin-angiotensin system (Renia-angiotensin system, RAS) and kallikrein-kinin system (Kallikrein-kinin system, KKS) are two important adjustment system. Among them, angiotensin-converting enzyme (ACE) is an enzyme that plays a key role in the system regulation process and is the main factor that increases blood pressure. Inhibiting the activity of ACE can effectively prevent and treat the occurrence of hypertension. [0003] It has been found that some foods contain hypotensive peptides, but the types are limited, and fermented foods produced by microbial methods can solve the problem that antihypertensive peptides with ACE inhibitory activity are limited by food sources and food varieties. Develop healthy food with antihyp...

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Application Information

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IPC IPC(8): C12N1/20C12R1/10
Inventor 俞龙浩王欣李艳青薛冰孟质文
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY