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Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof

A 4-D, herbicide technology, applied in the field of herbicide 2,4-D-insecticide CHI dual detection card and its preparation field, can solve the problems of high analysis cost, low efficiency, unfavorable popularization and use, etc. Accurate results and simple methods

Active Publication Date: 2013-04-17
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the analysis methods of environmental hormone pollutants are mainly divided into instrumental analysis methods represented by GC-MS, HPLC and LC-MS technologies, and immunoassay methods based on antigen-antibody reactions. These conventional residual analysis methods usually have complicated operating procedures. The efficiency is low, the analysis cost is high, it is not conducive to popularization and use, and it is difficult to adapt to the requirements of a large number of samples and on-site testing

Method used

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  • Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof
  • Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof
  • Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] figure 1 It is the structural diagram of the herbicide 2,4-D-insecticide CHI dual detection card of the present invention, figure 2 It is a cross-sectional structure diagram of a test strip of the herbicide 2,4-D-insecticide CHI dual detection card of the present invention, in which 10 is a PVC backing; 8 is a sample pad; 9 is a colloidal gold bonding pad, the The colloidal gold binding pad is coated with 2,4-D monoclonal antibody and CHI monoclonal antibody colloidal gold marker; 11 is the coating membrane, that is, the nitrocellulose membrane, and the nitrocellulose membrane is coated with 2,4-D Protein conjugates, CHI protein conjugates, and rabbit anti-mouse IgG.

[0023] A sample pad 8 and a colloidal gold bonding pad 9 are adhered to one end of the PVC backing 10 (sample loading end), and the sample pad 8 is lapped on the colloidal gold bonding pad 9 .

[0024] In the middle of the PVC backing 10 is adhered a nitrocellulose membrane 11 . A 2,4-D protein conjug...

Embodiment 2

[0027] The preparation of herbicide 2,4-D and insecticide CHI dual colloidal gold detection card is realized by the following steps:

[0028] (1) Preparation of colloidal gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 O) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated to boil, then added 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2O) Solution 5-7 mL, continue to stir and heat, when the color of the solution turns completely transparent purple red, stop heating after maintaining for 5 minutes, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0029] (2) Antibody pretreatment: centrifuge the 2,4-D or CHI monoclonal antibody to be labeled at 1000 r / min, 4°C for 20 min, take the supernatant, and dilute it to 1 mg with 0.01 mol / L PBS / mL; or dilute the labeled 2,4-D or CHI monoclonal antibody to 1 mg / mL with 0.01 mol / L PBS, and pa...

Embodiment 3

[0036] Minimum detection limit test.

[0037] Prepare the standard herbicide 2,4-D and insecticide CHI standard solution concentration: 0, 5 ng / mL, 10 ng / mL, 15 ng / mL, 20 ng / mL; add 2 drops of the sample to On the sample hole of the detection card, observe the color development results of the detection line T and the quality control line C after 5 minutes. After multiple tests, the detection rate of 2,4-D and CHI was over 99%, and the minimum detection limit was less than 15 ng / mL.

[0038]

[0039] 。

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Abstract

The invention discloses a herbicide 2, 4-D and pesticide CHI bigeminy detection card and a preparation method thereof, relating to the technical field of environmental hormone detection. A test paper strip is arranged in an outer shell of the detection card, and the detection card consists of a PVC (Poly Vinyl Chloride) rubber plate, a sample pad, a colloidal gold combining pad, a wrapping membrane and a water absorbing pad, wherein the colloidal gold membrane is a glass fiber cellulose membrane containing 2, 4-D monoclonal antibody and CHI monoclonal antibody colloidal gold markers; the wrapping membrane is a nitric acid cellulose membrane; two detection lines T and a quality control line C are arranged on the wrapping membrane; the detection lines T are wrapped by a 2, 4-D protein conjugate and a CHI protein conjugate; and the quality control line C is wrapped by a rabbit anti-mouse IgG antibody. By utilizing the method, the herbicide 2, 4-D and pesticide CHI in a water sample can be detected at the same time, and the method is simple, convenient, rapid, and the result is accurate.

Description

technical field [0001] The invention relates to the technical field of environmental hormone detection, in particular to a herbicide 2,4-D-pesticide CHI dual detection card and a preparation method thereof. Background technique [0002] In recent years, scientists have successively discovered some substances that exist outside the biological body and have similar effects to human and biological endocrine hormones. These substances can simulate the function of endocrine hormones and cause biological endocrine system disorders. They are called environmental hormones. or environmental hormones. Environmental hormones have become the third largest environmental problem in the world after the ozone layer and global warming, and have become a hot topic in the field of environmental science research. [0003] Environmental hormones refer to chemical substances that interfere with the endocrine of organisms exogenously. Chemicals released into the environment as a result of human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 杜道林洪霞张祯薛永来
Owner JIANGSU WISE SCI & TECH DEV