Method for enhancing soil fertility by promoting straw degradation through protease

A soil fertility and protease technology, which is applied in the field of biological enzyme application and soil fertilizer, can solve the problems of affecting seedling growth, affecting the yield of later seasons, short interval, etc., and achieves the effects of low cost, alleviation of nitrogen starvation and simple method.

Inactive Publication Date: 2013-04-24
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the interval between rice-wheat rotation or wheat-corn rotation is short, and nitrogen fixation seriously affects the growth of seedlings, which in turn affects the yield of later seasons.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Choose AS1.398 neutral protease (Bacillus subtilis neutral protease), its protease activity is 58,820 U / g, the optimal reaction pH is 6.5-7.5, and the optimal reaction temperature is 35-45?C, purchased from Beijing Fangshan enzyme preparation Main plant.

[0025] The soil samples came from the paddy field soil in Wujing Town, Minhang District, Shanghai, and the rice straw and soil were taken from the same plot. Under the condition of daily average temperature of 25-29℃, the soil samples were treated with enzyme treatment method and control method respectively.

[0026] Enzyme treatment method:

[0027] (1) The rice straw is crushed into small sections with a length of 1 mm to obtain crushed straw; the amount of straw used is 333 kg / mu.

[0028] (2) Prepare AS1.398 neutral protease into a protease solution with a concentration of 5882U / g, and spray crushed straw. Mix the crushed straw and enzyme solution evenly. The protease activity in the enzyme solution sprayed per gram of...

example 2

[0034] Choose AS1.398 neutral protease (Bacillus subtilis neutral protease), protease activity is 58,820 U / g, purchased from Beijing Fangshan Enzyme Preparation General Factory.

[0035] The soil samples were from the paddy field soil in Wujing Town, Minhang District, Shanghai, and the wheat straw and soil were taken from the same plot. Under the condition of daily average temperature of 25-29 ℃, the soil samples were treated with enzyme treatment method and control method respectively.

[0036] Enzyme treatment method:

[0037] (1) The wheat straw is crushed into small segments with a length of 1 mm to obtain crushed straw; the amount of straw used is 333 kg / mu.

[0038] (2) Prepare AS1.398 neutral protease into a protease solution with a concentration of 5882U / g, and spray crushed straw. Mix the crushed straw and enzyme solution evenly. The protease activity in the enzyme solution sprayed per gram of crushed straw is 588U.

[0039] (3) Bury the crushed straws sprayed with enzyme so...

example 3

[0044] The selected protease was AS1.398 neutral protease (Bacillus subtilis neutral protease) with an activity of 50,000 U / g, purchased from Beijing Fangshan Enzyme Preparation General Factory.

[0045] The soil samples came from the test site of Shandong Agricultural University in Tai'an City, Shandong Province, and the corn stalks and soil were taken from the same plot. Under the condition of daily average temperature of 20-25 ℃ (in the greenhouse), the soil samples were treated with enzyme treatment and control treatment respectively.

[0046] The enzyme treatment method is as follows:

[0047] (1) Cut the corn stalks into small sections with a length of 2 cm to obtain crushed stalks; the use ratio of stalks is 500 kg / mu.

[0048] (2) Prepare AS1.398 neutral protease into a protease solution with a concentration of 5000 U / g, and spray crushed straw. Mix the crushed straw and enzyme solution evenly. The protease activity in the enzyme solution sprayed per gram of crushed straw is...

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Abstract

The invention discloses a method for enhancing soil fertility by promoting straw degradation through protease, and relates to the technical field of bio-enzyme application and soil fertilizer. The method comprises the steps of at daily mean temperature of 20-29 DEG C, (1) slicing and grinding the straw into small sections of 0.1-8cm to obtain ground straw; (2) spraying a protease solution onto the ground straw; and (3) embedding the ground straw sprayed with the protease solution into the soil. Through returning the straw added with the protease solution into the field, the method for enhancing the soil fertility disclosed by the invention solves the problems of reduced nitrogen content, barrier to crop growth and the like appearing in the early stage of returning the straw into the field, and improves the nitrogen content in the soil, enhances soil fertility, improves crop growth environment, turns waste into valuable things, and reduces the cost.

Description

technical field [0001] The invention relates to the technical field of biological enzyme application and soil fertilizer, in particular to a method for enhancing soil fertility by using protease to promote straw degradation. Background technique [0002] my country is a large agricultural country with abundant straw resources. The annual output of straw in my country is about 700 million tons. Straw returning to the field is an important part of ecological agriculture and circular agriculture. However, in recent years, with the development of the rural economy, clean and convenient petrochemical energy has replaced traditional crop straws. About 2 / 3 of the total straw is burned directly in the country every year, which accelerates soil degradation and causes soil to compensate for the lack of organic matter. And soil failure, soil ecological environment into a vicious circle. The pollution of chemical fertilizers to the environment is becoming more and more serious, resulti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/00
Inventor 韩玮何明聂俊华
Owner NANJING UNIV OF INFORMATION SCI & TECH
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