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Method for enlarging pore diameter of yeast cell microcapsule wall

A technology of microcapsule wall material and yeast cell wall, which is applied in the direction of being fixed on or in biological cells, can solve the problems of low specific toxicity of polypeptide and protein drugs, and oral bioavailability of less than 1%, and achieves a reduction in production. Cost, increased pore size, prolonged in vitro activity effect

Inactive Publication Date: 2013-04-24
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Polypeptide protein drugs have the characteristics of high activity, strong specificity, and low toxicity, and can effectively treat difficult diseases such as cancer, autoimmune diseases, and hypertension, but their oral bioavailability is usually lower than 1%. Injection-based

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Yeast cells were used as the starting strain, and after slant culture and seed culture on potato medium, they were inoculated in fermentation medium for shaking flask culture for 48 hours. The obtained yeast cells were centrifuged, washed, resuspended in 0.5-10% hydrochloric acid solution, shaken at a temperature of 40-60°C for 48 h, centrifuged at 12,000 rpm for 10 min, and washed with water twice or more. Resuspend the autolyzed yeast cells in 0.1-5% mannanase solution (provided by Changsha Xiangzi Biotechnology Co., Ltd., 5000U / g), and perform shaking treatment at a temperature of 20-85°C for 24-72 After one hour, centrifuge at 12000 rpm for 10 min, wash with water more than twice, and freeze-dry to obtain the yeast cell microcapsule wall material with enlarged pore diameter. After mixing 20 g of yeast cell wall material and 6 g of solid SOD enzyme, add 260 g of water to stir, so that the yeast cell wall material and SOD enzyme solution are in high-frequency contact,...

Embodiment 2

[0032] Yeast cells were used as the starting strain, and after slant culture and seed culture on potato medium, they were inoculated in fermentation medium for shaking flask culture for 48 hours. The obtained yeast cells were centrifuged, washed, resuspended in 0.5-10% sodium hydroxide solution, shaken at a temperature of 40-60°C for 24 h, centrifuged at 12,000 rpm for 10 min, and washed with water twice or more. Resuspend the autolyzed yeast cells in 0.5-5% dextranase solution (provided by Changsha Xiangzi Biotechnology Co., Ltd., 3500U / g), and perform shaking treatment at a temperature of 20-85°C for 24-72 After one hour, centrifuge at 12000 rpm for 10 min, wash with water more than twice, and freeze-dry to obtain the yeast cell microcapsule wall material with enlarged pore diameter. After mixing 30 g of yeast cell wall material and 12 g of solid SOD enzyme, add 420 g of water to stir, so that the yeast cell wall material and SOD enzyme solution are in high-frequency contact...

Embodiment 3

[0034]Yeast cells were used as the starting strain, and after slant culture and seed culture on potato medium, they were inoculated in fermentation medium for shaking flask culture for 48 hours. The obtained yeast cells were centrifuged, washed, resuspended in 0.5-10% potassium chloride solution, shaken at a temperature of 40-60°C for 36 h, centrifuged at 12,000 rpm for 10 min, and washed with water twice or more. Resuspend the autolyzed yeast cells in 0.5-5% dextranase solution (provided by Changsha Xiangzi Biotechnology Co., Ltd., 3500U / g), and perform shaking treatment at a temperature of 20-85°C for 24-72 After one hour, centrifuge at 12000 rpm for 10 min, wash with water more than twice, and freeze-dry to obtain the yeast cell microcapsule wall material with enlarged pore diameter. After mixing 25 g of yeast cell wall material and 8 g of solid SOD enzyme, add 330 g of water for stirring, so that the yeast cell wall material and SOD enzyme solution are in high-frequency co...

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Abstract

The invention relates to a method for enlarging the pore diameter of a yeast cell microcapsule wall. A right amount of pore-enlarging agent is added to process the yeast cell wall to cause macromolecular substance to enter into the cell walls. Raw materials and equipment used in the method are familiar common raw materials and equipment, so that the dependence on expensive raw materials and instruments is avoided in the industrial production process, and the production cost is greatly reduced. Mannanase is adopted to degrade part of yeast cell wall, the pore diameter of a yeast cell can be effectively enlarged in the interval ultrasonic processing, the yeast cell wall on the same part cannot be exceedingly continuously hydrolyzed, and the pore diameter of the whole yeast cell wall is effectively controlled. The method has simple operation, the in vitro activity of macromolecule proteins is greatly prolonged, the stability of the macromolecule proteins is ensured, the production cost is reduced, and the production process is simplified.

Description

Technical field: [0001] The invention relates to a method for increasing the pore diameter of yeast cell microcapsule wall material, which belongs to the technical field of biological material processing. Background technique [0002] As a new technology that can improve the performance of functional substances, microcapsules have broad application prospects. Yeast cells are uniform in size, easy to cultivate, and cheap. The double-layer cyst structure composed of cell wall and cell membrane is a good material for embedding substances. French patent FR2179528 reported that industrial yeast was treated with a plasmolytic agent and embedded with neodymium chloride, magnesium chloride and onion extract; U.S. patent USA 4001480 introduced a method using fat cultured on a low-nitrogen high-carbon source medium A method for embedding oil-soluble compounds in yeast cells with a content as high as 40-60%; Patent EP-0085805A1 uses a public solvent that can be dissolved in both oil a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/16
Inventor 石国荣
Owner HUNAN AGRICULTURAL UNIV
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