Method for improving germination rate of wild hemp seeds
A hemp seed and germination rate technology, applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problem of wild hemp non-germination germination rate, etc., and achieve the effect of easy batch processing, neat germination and seedling emergence, and a wide range of applications
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Embodiment 1
[0023] (1) Harvest mature wild hemp seeds in Northeast China, thresh, remove impurities, and store them in the room at room temperature for 6 months after drying. Select plump and undamaged wild hemp seeds.
[0024] (2) Use the selected seeds with 1% H 2 o 2 Soak the aqueous solution at room temperature for 5 hours, take it out and rinse it with sterile water for 3 to 5 times, the soaking ratio is: use 200ml of diluted H for every 100g of seeds 2 o 2 aqueous solution.
[0025] (3) Bury the seeds treated in step 2 in wet sand at a temperature of 4°C, and add a small amount of water every 6 to 7 days to keep the absolute water content of the sand layer at 5 to 10%.
[0026] (4) After 20 days of cold-wet stratification treatment, remove the sand and rinse with sterile water for 3 to 5 times; take a clean petri dish with a diameter of 12 cm as a germination bed, and use double-layer filter paper germination method for germination culture. After the seeds in the bed are moisten...
Embodiment 2
[0028] (1) Harvest the mature wild hemp seeds in Yunnan, thresh, remove impurities, and store them in the room at room temperature for 30 days after drying. Select plump and undamaged wild hemp seeds.
[0029] (2) Use the selected seeds with 0.1%, 0.5%, 1% H 2 o 2 Soak the aqueous solution at room temperature for 5 or 24 hours, take it out and rinse it with sterile water for 3 to 5 times, the soaking ratio is: use 200 ml of diluted H for every 100 grams of seeds 2 o 2 aqueous solution.
[0030] (3) Bury the seeds treated in step 2 in wet sand at a temperature of 6°C. From the 7th day, observe whether the seeds have cracks, and observe once every other day. When the seeds are observed to have 40-50% of the seeds Split, and the radicle is exposed (that is, Lubai) for the next step of germination culture. During the treatment period, add a small amount of water every 6-7 days to keep the absolute water content of the sand layer at 5-10%.
[0031] (4) Remove the sand and rins...
Embodiment 3
[0035] The difference from Example 1 is that:
[0036] The cold-wet stratification treatment was: -20, 1, 4, 6°C for 20 days or -20°C for 7 days and then 4°C for 13 days. The germination results are shown in Table 2.
[0037] Table 2. Germination of wild cannabis under different cold-wet stratification temperatures
[0038] Processing number
[0039] 1
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