Molecular motor biosensor kit for detecting rotavirus

Abstract

The invention relates to a molecular motor biosensor kit for detecting food-borne rotavirus, belonging to the field of quick detection of food-borne viruses. The invention mainly solves the problems of overlong period and expensive instrument and equipment in the traditional detection. The core technique is to utilize an F0F1-ATPase molecular motor biosensor on chromatophore; and since the F0F1-ATPase can quickly rotate, coupling between the catalytic site and the proton transfer is established through the rotation. A VP7 probe is connected to an epsilon subunit of the ATPase; after a sample to be detected and a negative control are respectively combined with the biosensor, the ATP synthesis amounts under the catalytic action after 10 minutes are compared; and the ATP synthesis amount can be measured according to the amount of H<+> in the environment, and the amount of H<+> is acquired according to the fluorescence intensity embodied by the H-DHPE. The kit can be used for quickly and sensitively detecting food-borne rotavirus in a sample to be detected at high flux, and can effectively shorten the detection period and enhance the work efficiency.

Description

technical field [0001] The invention relates to a rapid detection technology for food-borne viruses. Background technique [0002] Rotavirus is the main pathogen of infantile diarrhea. The classic pathogen detection methods of rotavirus include electron microscope technique and isolation and culture. Electron microscopy requires that each milliliter of sample contains about 10 6 virus particles, and the virus particles are easy to degrade, which affects the correct diagnosis. The detection sensitivity of isolation and culture detection technology is low, requiring special culture conditions, and has limited value for basic research and clinical diagnosis of rotavirus. Antigen-based detection methods have low specificity and sensitivity, and cannot be effectively quantified. PCR technology has higher sensitivity and does not require cell culture, but the detection cost is high, and the operation steps are cumbersome and take a long time. Among the currently developed rot...

AI Technical Summary

Problems solved by technology

Antigen-based assays have low specificity and sensitivity, and cannot be effectively quantified

PCR technology has higher sensitivity and does not require cell culture, but the detection cost is high, and the operation steps are cumbersome and take a long time

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