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Analysis and identification method for riboflavin in tobacco

An identification method and riboflavin technology, applied in the field of cigarette detection and analysis, can solve problems such as limited qualitative analysis ability, and achieve the effects of excellent selectivity, shortening analysis time, and improving analysis efficiency

Active Publication Date: 2014-07-02
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leporati et al. used triple quadrupole tandem mass spectrometry to analyze a variety of water-soluble vitamins, but triple quadrupole tandem mass spectrometry can only obtain ion fragment information at the level of secondary mass spectrometry, and its qualitative analysis capability is limited

Method used

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  • Analysis and identification method for riboflavin in tobacco
  • Analysis and identification method for riboflavin in tobacco
  • Analysis and identification method for riboflavin in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Analysis and Identification of Riboflavin in Tobacco Samples

[0041] (one) Instruments, materials and reagents

[0042] The liquid chromatography-mass spectrometer consists of the Finnigan Surveyor liquid chromatography system and the Finningan LTQ linear ion trap mass spectrometer of Thermo Company in the United States. Dell computer with Xcalibur version 1.4 for instrument control, data acquisition and processing; Italian Claind LC-MS nitrogen generator; American Milli-Q ultrapure water instrument; German Memmert water bath oscillator; electronic balance Sartorius BS 423S (sensitivity 0.001g ); Whatman 0.2 μm nylon membrane needle filter.

[0043] Riboflavin (purity ≥ 98.0%) was purchased from Fluka Company, chromatographically pure methanol and formic acid were purchased from Merck Company and Teadia Company, Milli-Q grade water, and other reagents were of analytical grade.

[0044] (2) Experimental method

[0045] S1. Preparation of samples to be teste...

Embodiment 2

[0055] Example 2 Quantitative analysis of riboflavin in tobacco samples

[0056] (1) Instruments, materials and reagents are the same as in Example 1

[0057] (2) Experimental method

[0058] S1. Preparation of samples to be tested Weigh 0.25 g of tobacco powder, shake with 25 mL of 0.1 mol / L hydrochloric acid in a 95°C water bath for 1 hour, add 20 mL of 2 mol / L ammonium acetate after cooling, and dilute to 50 mL with water.

[0059] S2. Preparation of working standard solution The stock solution was prepared with methanol and stored at 2°C in the dark. Prepare a 4 μg / mL working standard solution with water, store it in the dark at 2°C immediately after use, and prepare it every two days. The riboflavin in the sample was determined by the standard addition method, and 5 concentration gradients were prepared with the sample solution to be tested treated by S1, and the sample was directly injected after filtering through a 0.2 μm filter membrane.

[0060] The concentration ...

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Abstract

The invention relates to the technical field of cigarette detection and provides an analysis and identification method for riboflavin in tobacco. Aiming at riboflavin in tobacco samples, the present invention establishes a liquid chromatography-electrospray linear ion trap multistage mass spectrometry method (LC-ITMS2 method) for sensitive, accurate and rapid determination of riboflavin in tobacco samples, and successfully realizes the detection of riboflavin in tobacco samples Assay. The detection method of the present invention has a linear range of 8-10000 ng / mL, a correlation coefficient R2 greater than 0.99, a detection limit of 0.5 ng / mL (S / N=3), and a precision of less than 3%. The method of the present invention avoids the problem of co-elution, has high sensitivity, excellent selectivity, simplifies the lengthy and complicated sample pretreatment, effectively shortens the analysis time, greatly improves the analysis and detection efficiency, and greatly improves the analysis and identification efficiency. From the perspective of analysis, it was confirmed that riboflavin is a trace component contained in tobacco itself.

Description

technical field [0001] The present invention relates to the technical field of cigarette detection and analysis, more specifically, to an analysis and identification method for riboflavin in tobacco, especially to the analysis and identification of riboflavin in tobacco by using liquid chromatography-electrospray linear ion trap multi-stage mass spectrometry analysis method. flavin. Background technique [0002] Riboflavin, the B vitamin 2 , is a water-soluble compound. Riboflavin has a variety of biological and physiological functions, and is an indispensable micronutrient for maintaining human health. For example, a lack of riboflavin can cause oral inflammation, glossitis, and corneal inflammation. Riboflavin in tobacco was discovered in the late 1950s. The research on riboflavin in tobacco-related fields has attracted the attention of many scholars and research institutions. It can be used as an antioxidant in the body, which is beneficial to prevent the conversion of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 黄翼飞胡静
Owner CHINA TOBACCO GUANGDONG IND