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Method for separating small molecule peptides from soybeans and for preparing small molecule peptides and cosmetic composition

A cosmetic composition and small molecular peptide technology, which can be used in cosmetics, cosmetic preparations, cosmetic preparations, etc., can solve problems such as reducing serum cholesterol concentration, achieve excellent skin wrinkle improvement effect, promote and inhibit collagenase expression, inhibit Excellent effect

Inactive Publication Date: 2013-06-05
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, oligopeptides with a molecular weight of more than 1,000 Da have been reported to combine with bile acids and cholesterol in the small intestine to be excreted, and have the effect of reducing serum cholesterol concentration and physiological activities such as anti-tumor, anti-hypertension, and immune enhancement. No reports on skin antioxidant and anti-aging effects

Method used

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  • Method for separating small molecule peptides from soybeans and for preparing small molecule peptides and cosmetic composition
  • Method for separating small molecule peptides from soybeans and for preparing small molecule peptides and cosmetic composition
  • Method for separating small molecule peptides from soybeans and for preparing small molecule peptides and cosmetic composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 is derived from the preparation of the small molecule peptide extract of soybean cake

[0035] 1 kg of soybean cake prepared from soybeans and 3 L of purified water were added to a container and mixed and stirred. The resulting mixture was then filtered through a filter cloth to separate the residue and filtrate, and the separated filtrate was concentrated under reduced pressure. The concentrated extract was heat-treated at 100°C for 20 minutes, and the heat-treated product was centrifuged to separate the residue and filtrate. The obtained filtrate was dried to obtain 12 g of small molecular peptide extract derived from soybean cake.

experiment example 2

[0043] Experimental Example 2 Antioxidant Effect Experiment (DPPH Test)

[0044] The DPPH oxidation inhibitory effect of the small molecule peptide extract derived from soybean cake in Example 1 and the natural product extract in Comparative Example 1-2 was compared with water-soluble vitamin E to determine.

[0045] Antioxidation was evaluated using the change in absorbance by reduction of the organic radical DPPH (1,1-diphenyl-2-picryl hydrazyl) (antioxidant oxidation) ability method. The oxidation of the DPPH of the extracts of Example 1 and Comparative Examples 1-2 obtained above was measured to be inhibited and the degree to which the absorbance was reduced compared to the control group, and the concentration showing an absorbance of 50% or less compared to the absorbance of the control group was regarded as the effective concentration. Antioxidant concentrations were evaluated.

[0046] Add 190 μl of 100 μM (in ethanol) DPPH solution and 10 μl each of the examples, com...

experiment example 3

[0051] Experimental Example 3 Collagenase Expression Inhibition Effect Determination

[0052] The collagenase expression inhibition of the small molecule peptide extract derived from soybean cake in Example 1 and the natural product extract in Comparative Example 1-2 can be determined by comparing with tocopherol and EGCG. The lower the expression level of collagenase, the higher the ability to inhibit the expression of collagenase, the less the decomposition of collagen in the skin, and therefore the less the amount of wrinkles generated. In addition, tocopherol and EGCG are antioxidant substances known to have the function of regenerating skin epidermal cells and preventing skin aging.

[0053] Human fibroblasts were added at 5,000 cells / well in a 96-well microtiter plate in DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% fetal bovine serum, Culture until 90% growth. Thereafter, after cultivating in serum-free DMEM medium for 24 hours, using the comparative...

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Abstract

The invention relates to a method for separating small molecule peptides from soybeans and for preparing small molecule peptides and a cosmetic composition and specifically relates to a cosmetic composition which mainly contains small molecule peptide extractives separated from soybean cakes and therefore the cosmetic composition has antioxidant and anti-aging effects.

Description

technical field [0001] The present invention relates to the separation and preparation of small molecule peptide extracts and cosmetic compositions thereof from soybeans, in particular, relates to mainly containing soybean cakes The small molecular peptide extract isolated from the product is a cosmetic composition with anti-oxidation and anti-aging effects. Background technique [0002] Active oxygen is generated due to various physical, chemical and environmental factors such as enzyme systems, reduction metabolism, chemicals, pollution substances and photochemical reactions in the body, and produces non-selective effects on cell components such as lipids, proteins, sugars and DNA. The irreversible destructive effect can cause various diseases such as cell aging or cancer. Moreover, various peroxides in the body, such as lipid peroxides produced by lipid peroxidation caused by these reactive oxygen species, also cause oxidative damage to cells and cause various dysfuncti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64C07K1/14A61Q19/08
CPCA61K8/64A61K8/645A61K8/9789A61Q19/00A61Q19/08
Inventor 沈晋燮朴葰星黄景焕姜永圭廉明勋曹濬喆
Owner AMOREPACIFIC CORP
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