Tumor cell active targeting drug delivery system based on nanometer particles and construction method thereof

A nanoparticle and active targeting technology, which is applied in the direction of antineoplastic drugs, pharmaceutical formulations, drug combinations, etc., can solve the problems of no research reports, etc., and achieve the effects of reduced toxicity, no immunogenicity, and good stability

Active Publication Date: 2013-06-05
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no related research has been reported

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  • Tumor cell active targeting drug delivery system based on nanometer particles and construction method thereof
  • Tumor cell active targeting drug delivery system based on nanometer particles and construction method thereof
  • Tumor cell active targeting drug delivery system based on nanometer particles and construction method thereof

Examples

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Effect test

Embodiment 1

[0037] Construction method of tumor cell-targeted drug delivery system based on titanium oxide-based nanoparticles

[0038] (1) Synthetic folic acid / TiO 2 Nanocomposite: Take TiO 2 Nanoparticle aqueous solution 2mL (TiO 2 It is a spherical titanium oxyhydroxide precursor, particle size 1-2nm, Ti molar concentration 0.1mM), and stirred for 0.5 hours under nitrogen gas; under stirring state, 0.1mL of 0.1mM folic acid solution was added dropwise, protected from light, and stirred for 24 hours to make Folic acid molecules are fully adsorbed to TiO 2 Nanoparticle surface; after stirring, transfer the mixture solution to a centrifuge tube and centrifuge at a speed of 10,000 rpm for 10 minutes. After centrifugation, collect the unadsorbed folic acid in the supernatant, and then add The same amount of ultrapure water was used to resuspend the precipitate, and the centrifugation was repeated three times to obtain folic acid / TiO 2 Complex.

[0039] (2) Synthesis of folic acid / TiO ...

Embodiment 2

[0041] Construction method of tumor cell-targeted drug delivery system based on titanium oxide-based nanoparticles

[0042] (1) Synthesis of TiO 2 / Epirubicin nanocomposite: take TiO 2 Nanoparticle aqueous solution 1mL (TiO 2 Rod-shaped, particle size 100nm, Ti molar concentration 1000mM), nitrogen gas and stirred for 12 hours; under stirring, 2mL epirubicin solution (10mM) was added dropwise to TiO 2 Nanoparticle aqueous solution, protected from light, stirred for 24 hours; after stirring, the mixture solution was transferred to a centrifuge tube for centrifugation at a speed of 15,000 rpm, and the centrifugation time was 30 minutes. After centrifugation, the unadsorbed surface in the supernatant was collected. rubicin, then add the same amount of ultrapure water as the supernatant, resuspend the precipitate, and repeat centrifugation three times to obtain TiO 2 / Epirubicin Nanocomplex.

[0043] (2) Synthesis of folic acid / TiO 2 / epirubicin nanocomposite: the TiO obta...

Embodiment 3

[0045] Construction method of tumor cell-targeted drug delivery system based on titanium oxide-based nanoparticles

[0046] (1) Synthesis of aminopterin / TiO 2 Nanocomposite: Take TiO 2 Nanoparticle aqueous solution 1mL (TiO 2 Spherical anatase, particle size 10nm, Ti molar concentration 500mM), stirred for 1 hour under nitrogen gas; under stirring state, gradually added dropwise 0.5mL of 50mM aminopterin solution, protected from light, and stirred for 72 hours to make the aminopterin molecules Fully adsorbed to TiO 2 surface of nanoparticles; after stirring, transfer the mixture solution to a centrifuge tube for centrifugation, the centrifugation speed is 10,000 rpm, and the centrifugation time is 20 minutes. After centrifugation, the unadsorbed aminopterin in the supernatant is collected, and then added with The same amount of ultrapure water as the supernatant was used to resuspend the pellet, and the centrifugation was repeated three times to obtain the aminopterin / TiO ...

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Abstract

The invention relates to a tumor cell active targeting drug delivery system based on nanometer particles and a construction method thereof. The tumor cell active targeting drug delivery system is characterized by using the nanometer particles with photocatalytic performance as carriers to obtain targeted molecule/nanometer particle/chemotherapy drug compounds in a physical absorption or chemical bonding mode, the mole ratio of the nanometer particles and targeted molecules is 50:1-20:1, the mole ratio of the nanometer particles and chemotherapy drugs is 50:1-10:1. The tumor cell active targeting drug delivery system integrates the chemotherapy drugs, the targeted molecules and the nanometers particles with photocatalytic performance, enables a nanometer particle dry carrying system to have active tumor cell targeting capacity, effectively reduces side effects of the chemotherapy drugs, can realize controllable release of the chemotherapy drug and reverse multiple drug resistance of the tumor cell, and is simple and easy in construction method, low in cost, and favorable to industrialized production and market popularization.

Description

technical field [0001] The invention belongs to the interdisciplinary field of nanomaterials and biomedical sciences, and relates to a nanoparticle-based active targeted drug delivery system for tumor cells and a construction method thereof. The system is used to realize the controllable release of chemotherapy drugs and reverse the multidrug resistance of tumor cells sex. Background technique [0002] Malignant tumors are the most serious diseases threatening human health. At present, several methods such as surgery, radiotherapy, chemotherapy, and traditional Chinese medicine are mainly used to treat malignant tumors. Among them, chemotherapy, as a systemic treatment, has a positive effect on killing tumor cells and inhibiting tumor spread. However, traditional chemotherapeutic drugs are cytotoxic drugs. While killing tumor cells, they also damage normal cells to varying degrees, especially the vigorously growing blood and lymphocytes in the human body, destroying the hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/704A61K47/22A61K47/42A61K47/48A61P35/00
Inventor 任文智吴爱国
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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