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Method for increasing activity of microbial flocculant

A microbial flocculant and flocculant technology, applied in separation methods, chemical instruments and methods, flocculation/sedimentation water/sewage treatment, etc., can solve the problems of unstable microbial flocculant production technology, high cost, secondary pollution, etc. Achieve the effect of simple operation, remarkable effect and broad application prospect

Active Publication Date: 2013-06-05
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These technologies lead to high cost, secondary pollution, unstable microbial flocculant production technology, etc.

Method used

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  • Method for increasing activity of microbial flocculant
  • Method for increasing activity of microbial flocculant
  • Method for increasing activity of microbial flocculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 microbial flocculant

[0031] 1. Microbial flocculant fermentation method

[0032] Medium A was used for the activation and fermentation of Brevibacillus brevis CGMCC1.931, and Medium B was used for the activation and fermentation of Rhodococcus erythropolis CGMCC1.2362. After the strains were activated, they were transferred to (30mL / 300mL) Erlenmeyer flasks of corresponding fermentation medium according to the inoculated amount of 1% respectively, and then fermented with flocculants at 30°C, 120rpm, and fermented for 96h.

[0033] 2. Microbial flocculant preparation

[0034]Brevibacillus CGMCC1.931 polysaccharide flocculant preparation: fermentation broth 100mL, 10000×g, 4°C, centrifuged for 15min, and the supernatant was taken. The supernatant was concentrated in a vacuum concentrator to 1 / 4 of the original volume. Add 3 times the volume of absolute ethanol and let stand at -20°C overnight. Centrifuge at 10,000×g at 4°C for 15 minu...

Embodiment 2 4

[0036] Example 2 The stress-resistance enhancement pretreatment of microbial flocculants by ectoine substances

[0037] 1. Flocculant sample preparation:

[0038] Sample a: 1 g of dry Bacillus brevis CGMCC1.931 flocculant powder prepared by the method described in Example 1 was dissolved in 10 mL of deionized water to prepare a concentration of 0.1 g / mL.

[0039] Samples b1-b3: flocculants treated with ectoine stress-enhancing treatment (sample a was prepared with 0.1, 1, and 10 g / L ectoine, and placed at room temperature for 30 minutes before use).

[0040] Samples c1~c3: flocculants treated with hydroxyectoine stress-enhancing treatment (sample a was prepared with 0.1, 1, 10 g / L hydroxyectoine, and used after standing at room temperature for 30 minutes). The flocculant sample preparation is shown in Table 1.

[0041] Table 1 Flocculant sample preparation

[0042]

[0043] 2. Determination method of flocculation rate:

[0044] Under the stirring state of 120rpm, add 0....

Embodiment 3

[0051] Example 3 ectoine improves the flocculation activity of microbial flocculants at high temperatures

[0052] Rhodococcus erythropolis CGMCC1.2362 produces protein flocculants [Wang Lan, Tang Jing, Zhao Xuan, Research methods for the flocculation mechanism of microbial flocculants, Journal of Environmental Engineering Vol.5, No.3, March 2011]. The structure of protein flocculants is unstable at high temperature, and the flocculation activity is significantly reduced. This example is used to prove that the use of ectoine can protect the flocculation activity of protein flocculants at high temperatures, and the specific methods are as follows:

[0053] Prepare 2 parts of kaolin suspension (0.5g of kaolin is added to 100mL of distilled water), one of which is added with 0.5mL of Rhodococcus erythropolis CGMCC1.2362 flocculant (the dry powder of Rhodococcus erythropolis CGMCC1.2362 flocculant prepared by the method described in Example 1 1g, dissolved in 10mL deionized water...

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Abstract

The invention relates to a method for increasing activity of a microbial flocculant, which is characterized in that Ectoine or tetrahydropyrimidine is added, the stable conformation of the microbial flocculant is protected, and the flocculant activity can be enhanced. The method provided by the invention solves the problem that when the microbial flocculant, is used for treating waste water with high ion concentration, high concentration heavy metal, extreme pH, and high / low temperature, the flocculation activity of the microbial flocculant is obviously reduced. The method for increasing activity of the microbial flocculant has application prospect on treatment of water and waste water, fermentation and food postprocessing.

Description

technical field [0001] The invention belongs to the technical field of microbial flocculants, and in particular relates to a method for improving the flocculation activity of microbial flocculants. Background technique [0002] Microbial flocculants (Microbial Flocculants, MBF) are macromolecular substances with flocculation activity produced by microorganisms. Compared with the currently widely used inorganic flocculants and artificially synthesized organic polymer flocculants, microbial flocculants are highly efficient, non-toxic, non-environmental pollution, easy to biodegrade, no secondary pollution, and have the functions of flocculation, decolorization, complexation of heavy metals, etc. It is a new generation flocculant with various functions. Microbial flocculants have shown great application value and market prospects in the fields of water supply, sewage / wastewater treatment, fermentation and food post-processing. [0003] Microbial flocculants are divided into d...

Claims

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Application Information

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IPC IPC(8): C02F1/56B01D21/01
Inventor 张苓花朱益民陈箐
Owner DALIAN MARITIME UNIVERSITY
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