Method of improving concentration of butanol in process of acetone butanol fermentation

Abstract

The invention belongs to the field of the biochemical industry and particularly relates to a method of improving the concentration of butanol in the process of acetone butanol fermentation. The method of improving the concentration of the butanol in process of the acetone butanol fermentation includes the following steps of adding a surface active agent to a culture medium in the process of the acetone butanol fermentation and extracting and improving the concentration of the butanol by means of micelle formed by the surface active agent. The method of improving the concentration of butanol in the process of the acetone butanol fermentation is directed against the product inhibition effect existing in the process of the acetone butanol fermentation and extracts the butanol by means of the micelle formed by the surface active agent serving as an extraction agent, relieving the inhibition effect in the process of the acetone butanol fermentation, increases the final concentration of the butanol in fermentation liquor, and therefore energy consumption of rectification in a later period is reduced.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for increasing the concentration of butanol in the fermentation process of acetone butanol. Background technique [0002] Energy is the driving force of economic and social activities. Today, with the deepening of economic globalization, energy is no longer simply an economic issue of a country, but has become a global economic, political, diplomatic and even military issue. At present, the continuous rise in oil prices caused by the shortage of oil resources has become an irreversible trend. Whether it is a developed country or a developing country, the development and utilization of new energy are regarded as key research projects in key scientific and technological fields, and they attach importance to the rational use and regeneration of limited resources on the earth. [0003] my country's oil resources are relatively poor, with reserves of only 2% o...

AI Technical Summary

Problems solved by technology

Therefore, the fermented liquid saturated with extractant needs to be purified before reuse, and purifying this solution containing a small amount of extractant also needs to consume a lot of energy (the problem of separating butanol becomes the problem of separating extractant), if The fermented liquid saturated by the extractant cannot be reused, so the lost extractant will greatly increase the production cost of butanol, and even have a negative value, that is, the lost extractant may be more valuable than the obtained butanol; secondly, after Stirring and extracting the fermented liquid, due to the existence of dead bacteria, it is easy to form an emulsion. Once the emulsification phenomenon is formed, it will bring great difficulties to the separation operation; the gas stripping process has the following disadvantages. The selectivity of the stripping itself is low, the butanol content in the stripping gas blown from the fermenter is very low, and due to the low thermal conductivity of the gas, the energy consumption of the condensation process is relatively high, which limits the application of this process in the on-line separation of bio-butanol; On the other hand, the gas will produce a lot of bubbles in the butanol fermenter, most of the gas stripping process needs to add defoaming agent, and the defoaming agent has inhibitory effect on the cell activity

[0013] To sum up, the problem of high separation cost caused by low product concentration is the bottleneck problem restricting the large-scale production of butanol by fermentation method, and there is no good solution at present

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