Method of improving concentration of butanol in process of acetone butanol fermentation

An acetone-butanol fermentation and concentration technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, can solve the problems of high energy consumption in the condensation process, low butanol content, high separation costs, etc., to reduce distillation Energy consumption, increased final product concentration, and less dosage

Active Publication Date: 2013-06-05
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

Therefore, the fermented liquid saturated with extractant needs to be purified before reuse, and purifying this solution containing a small amount of extractant also needs to consume a lot of energy (the problem of separating butanol becomes the problem of separating extractant), if The fermented liquid saturated by the extractant cannot be reused, so the lost extractant will greatly increase the production cost of butanol, and even have a negative value, that is, the lost extractant may be more valuable than the obtained butanol; secondly, after Stirring and extracting the fermented liquid, due to the existence of dead bacteria, it is easy to form an emulsion. Once the emulsification phenomenon is formed, it will bring great difficulties to the separation operation; the gas stripping process has

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  • Method of improving concentration of butanol in process of acetone butanol fermentation
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  • Method of improving concentration of butanol in process of acetone butanol fermentation

Examples

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Embodiment 1

[0029] Embodiment 1 micellar toxicity test

[0030]The seed solution (Clostridium beijerinckiiY3) of the ABE (acetone, butanol, ethanol) fermentation process was anaerobically cultured at 37 degrees for 12 hours, and was inoculated into 30mL medium (see Table 1 for the medium composition) according to the 10% inoculum size of the seed solution. , different types of surfactants were added to the culture medium, including Tween 20, Tween 80, Tween 85, Span 80, Span 85, the addition amount was 2wt%, and cultured in a shaker at 150rpm / min for 12 hours, Utilize drainage method to measure the gas production in each shake flask, after fermentation carries out 24 hours, measure butanol concentration in each shake flask, do blank control simultaneously (referring to figure 2 ). Depend on figure 2 It can be seen from the figure that after 24 hours of fermentation under this culture condition, the concentration of butanol did not reach the inhibitory concentration, so the gas product...

Embodiment 2

[0034] Taking Tween 80 as an example, the effect of concentration is similar to that of micellar toxicity experiment. Tested by shake flask batch fermentation. The seed solution (Clostridium beijerinckii Y3) was anaerobically cultured at 37 degrees for 12 hours, and was inoculated into 10 mL of medium (see Table 1 for medium composition) according to 10% inoculum size, and different concentrations of Tween 80 were added to the medium. From 0-6%, cultivated in 150rpm / min shaker for 12 hours, utilized the drainage method to measure the gas production in each shake flask, after fermentation carried out 72 hours, measured the butanol concentration in each shake flask, and blank control was done simultaneously (see image 3 ). image 3 It can be seen that with the increase of surfactant addition, the gas production and butanol concentration of the product both increase. This indicated that surfactants could effectively solubilize butanol in the product, thereby weakening the inhi...

Embodiment 3

[0036] Configure a series of butanol aqueous solutions without surfactant, and use the headspace gas phase to measure the proportional relationship between the butanol concentration in the solution and the butanol chromatographic area in the gas above, as a standard curve (see Figure 4 ). Configure a series of butanol aqueous solution containing surfactant and known butanol concentration, according to the above-mentioned standard curve, compare the concentration contrast containing surfactant and not containing active agent (see Figure 5 ). Depend on Figure 4 It can be seen that the linear correlation between butanol concentration and chromatographic area in the standard curve is very high, indicating that the concentration of butanol solution can be converted by the gas concentration above the solution. Depend on Figure 5 It can be seen that for samples with different concentrations of butanol, the concentration change decreases with the increase of surfactant addition...

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Abstract

The invention belongs to the field of the biochemical industry and particularly relates to a method of improving the concentration of butanol in the process of acetone butanol fermentation. The method of improving the concentration of the butanol in process of the acetone butanol fermentation includes the following steps of adding a surface active agent to a culture medium in the process of the acetone butanol fermentation and extracting and improving the concentration of the butanol by means of micelle formed by the surface active agent. The method of improving the concentration of butanol in the process of the acetone butanol fermentation is directed against the product inhibition effect existing in the process of the acetone butanol fermentation and extracts the butanol by means of the micelle formed by the surface active agent serving as an extraction agent, relieving the inhibition effect in the process of the acetone butanol fermentation, increases the final concentration of the butanol in fermentation liquor, and therefore energy consumption of rectification in a later period is reduced.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for increasing the concentration of butanol in the fermentation process of acetone butanol. Background technique [0002] Energy is the driving force of economic and social activities. Today, with the deepening of economic globalization, energy is no longer simply an economic issue of a country, but has become a global economic, political, diplomatic and even military issue. At present, the continuous rise in oil prices caused by the shortage of oil resources has become an irreversible trend. Whether it is a developed country or a developing country, the development and utilization of new energy are regarded as key research projects in key scientific and technological fields, and they attach importance to the rational use and regeneration of limited resources on the earth. [0003] my country's oil resources are relatively poor, with reserves of only 2% o...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12P7/28C12R1/145
Inventor 侯同刚王立英张跃冬刘杰牟新东
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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