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Paenibacillus terrae biological agent and application thereof in agriculture

A technology of bacillus and biological preparations, applied in the field of biological preparations and its application in agriculture, can solve the problems of unresolved plant diseases, reduction of agricultural product yield and quality, environmental agricultural product pollution, etc., and achieve the suppression of fungal soil-borne diseases , reduce the amount of use, reduce the effect of pollution

Active Publication Date: 2013-06-12
HEILONGJIANG AGRIBUSINESS SANLONG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to statistics, in the production reduction of continuous cropping, the reduction caused by diseases accounts for more than 75%. However, agricultural producers can only rely on traditional control methods to control by continuously increasing the dosage and frequent use of pesticides. This not only fails to solve the problem of plant diseases, but brings Serious side effects, causing serious pollution to the environment and agricultural products, reducing the yield and quality of agricultural products

Method used

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  • Paenibacillus terrae biological agent and application thereof in agriculture
  • Paenibacillus terrae biological agent and application thereof in agriculture
  • Paenibacillus terrae biological agent and application thereof in agriculture

Examples

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specific Embodiment approach 1

[0015] Specific Embodiment 1: In this embodiment, Paenibacillus terrae biological preparations contain Paenibacillus terrae NK3-4; Paenibacillus terrae biological preparations are divided into Paenibacillus terrae liquid biological preparations and Paenibacillus terrae solid biological preparations preparation.

[0016] The Paenibacillus terrae NK3-4 in this embodiment is a Gram-positive bacterium, the bacterium is rod-shaped, 1.0-1.2 μm×3-4 μm, can form spores, and the proximal end of the bacterium expands when forming spores , the cyst is obviously inflated, the bud is oval, located at one end of the cyst (such as figure 1 shown). Paenibacillus terrae (Paenibacillus terrae) NK3-4 is cultured on a silicate sucrose nitrogen-free medium plate for 5 to 7 days to form a circular colony with a diameter of about 1 to 2 mm. Moist, translucent, with thick bacterial lawn, easy to stir up (such as figure 2 shown). Paenibacillus terrae (Paenibacillus terrae) NK3-4 colonies on the p...

specific Embodiment approach 2

[0027] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the liquid biological preparation of Paenibacillus terrae is a culture fermentation liquid containing Paenibacillus terrae (Paenibacillus terrae) NK3-4. Other steps and parameters are the same as those in Embodiment 1.

[0028] The liquid biological preparation of Paenibacillus terreus had obvious inhibitory effect on pathogenic Fusarium oxysporum. On the PDA medium inoculated with Fusarium oxysporum, the liquid biological preparation of Paenibacillus terreus was added dropwise for confrontation culture on a plate, and an obvious inhibition zone appeared.

[0029] In this embodiment, the number of spores in the Paenibacillus terreus liquid biological preparation is 1×10 9 ~2×10 9 Individual / ml, field crops use 3.5~9 L / hm of Paenibacillus terreus liquid biological preparation of the present invention 2 , can increase crop production by more than 8%, and combined with silicon fertilizer, can ...

specific Embodiment approach 3

[0030] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the solid biological preparation of Paenibacillus aeresis is mixed with the liquid biological preparation of Paenibacillus aerene as the main material, and then extruded and granulated to obtain Paenibacillus aerene Bacillus solid biological preparation. Other steps and parameters are the same as those in Embodiment 1 or 2.

[0031] In this embodiment, the use of the solid biological preparation of Paenibacillus aureus can not only increase the yield of crops, but also have the effect of reducing the amount of chemical fertilizer used.

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Abstract

The invention relates to a biological agent and application thereof in agriculture, particularly a Paenibacillus terrae biological agent and application thereof in agriculture. The Paenibacillus terrae biological agent contains Paenibacillus terrae NK3-4; and the Paenibacillus terrae biological agent is divided into a Paenibacillus terrae liquid biological agent and a Paenibacillus terrae solid biological agent. The Paenibacillus terrae liquid biological agent or Paenibacillus terrae solid biological agent can be applied. The invention is applicable to the field of agriculture.

Description

technical field [0001] The invention relates to a biological agent and its application in agriculture. Background technique [0002] Nitrogen, phosphorus, and potassium are macronutrient elements necessary for plant growth. The total phosphorus content in the soil cultivation layer is 0.1-2.0g / kg, of which inorganic phosphorus accounts for 15%-80% of the total phosphorus, and most of them are insoluble Inorganic phosphorus compounds are difficult to be directly used by plants; the total potassium content in the soil cultivation layer is 0.5-45g / kg, of which mineral potassium accounts for 90%-98% of the total potassium, which is difficult to be directly used by plants; applied in agricultural production The utilization rate of phosphorus and potassium fertilizers is only 40% to 60%, and the rest is refixed by the soil. Due to the low utilization rate of crops, it is easy to lose, causing serious waste and environmental pollution. [0003] Due to the continuous increase of hu...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P21/00A01P1/00A01P3/00C05G3/04C05G3/80
Inventor 于文清胡广民田艳洪赵晓锋刘文志李鹏隋文志
Owner HEILONGJIANG AGRIBUSINESS SANLONG BIOTECH
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