Molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of molecular markers and curly degree, applied in the field of animal molecular genetics, to achieve the effects of low cost, accelerated breeding process, and high accuracy
Inactive Publication Date: 2013-06-12
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF2 Cites 8 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, there are few reports on the res
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
specific Embodiment approach 1
[0019] Embodiment 1: In this embodiment, a molecular marker method capable of simultaneously predicting and identifying the fineness and crimp of sheep wool is carried out according to the following steps:
[0020] 1. Design a pair of primers MTRF1 and MTRR1 according to the G9542341A site of the 27th intron region of the sheep MTR gene, and then perform PCR amplification on the sheep genomic DNA to obtain the PCR amplification product, and then use the endonuclease MspI to digest the PCR amplification product, to obtain enzyme cleavage product;
[0021] 2. Use agarose gel with a concentration of 2% to 3% to electrophoresis separate the digested products, and then determine the genotype according to the electrophoresis separation results. The criteria for determination: ① Electrophoresis presents two bands, the size of which is 269bp and 209bp, When the G9542341A site in the 27th intron region of the sheep MTR gene is not mutated, the PCR amplification product can be completel...
specific Embodiment approach 2
[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the reaction system for PCR amplification in step one is a 10 μL reaction system, which consists of the following components:
[0032]
[0033]
[0034] PCR amplification conditions were: 94°C pre-denaturation for 5 min, 94°C denaturation for 30 s, 65°C annealing for 25 s, 72°C extension for 30 s, a total of 33 cycles, 72°C extension for 7 min, and 4°C incubation. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0035] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the enzyme digestion system in step one is as follows:
[0036]
[0037] The enzyme digestion conditions are: 37°C enzyme digestion for 1-2h or overnight. Others are the same as in the first embodiment.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool and relates to a molecular marking method. The method comprises the following steps: 1, designing a primer for performing amplification on sheep genome DNA (Deoxyribonucleic acid), and performing enzyme digestion, thereby obtaining an enzyme digestion product; 2, performing electrophoresis separation on the enzyme digestion product, and determining the genotype according to the electrophoresis separation result; 3, performing correlation analysis, and estimating the least squares mean value of the properties, wherein the results are that the wool fineness of the AA, GA genotype group in three genotypes is obviously lower than that of the GG genotype group, and the wool crimpness with the AA genotype group is obviously higher than that of the GG genotype group; and 4, dividing the experimental group into three types, and finishing the operation. The fineness and crimpness of sheep wool of the sheep can be simultaneously predicted and identified, an effective, simple and feasible molecular marking method is provided for quality character improvement and auxiliary mark selection of the sheep wool, and the method can be effectively applied to the field of molecular assisted breeding for superfine wool sheep.
Description
technical field [0001] The invention relates to the field of animal molecular genetics, in particular to a method for simultaneously detecting the fineness and crimp of sheep wool with molecular markers. Background technique [0002] Fine-wool sheep occupy an important position in my country's animal husbandry industry, and the main product of fine-wool sheep is wool. As an important textile raw material, fine wool has high economic value. The production of fine-wool sheep is not only related to the economic development and social stability of the producing areas, but also to the development of my country's wool spinning industry and the balance of import and export trade. With the increasing demand for wool at home and abroad, the cultivation of high-quality fine-wool sheep has become an urgent problem to be solved in the field of wool production and sheep breeding. [0003] The breeding of Chinese Merino sheep (Xinjiang military reclamation type) began in 1972, and has s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more