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Molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool

A technology of molecular markers and curly degree, applied in the field of animal molecular genetics, to achieve the effects of low cost, accelerated breeding process, and high accuracy

Inactive Publication Date: 2013-06-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the research of MTR gene in livestock and poultry

Method used

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  • Molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool
  • Molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool
  • Molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool

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Experimental program
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specific Embodiment approach 1

[0019] Embodiment 1: In this embodiment, a molecular marker method capable of simultaneously predicting and identifying the fineness and crimp of sheep wool is carried out according to the following steps:

[0020] 1. Design a pair of primers MTRF1 and MTRR1 according to the G9542341A site of the 27th intron region of the sheep MTR gene, and then perform PCR amplification on the sheep genomic DNA to obtain the PCR amplification product, and then use the endonuclease MspI to digest the PCR amplification product, to obtain enzyme cleavage product;

[0021] 2. Use agarose gel with a concentration of 2% to 3% to electrophoresis separate the digested products, and then determine the genotype according to the electrophoresis separation results. The criteria for determination: ① Electrophoresis presents two bands, the size of which is 269bp and 209bp, When the G9542341A site in the 27th intron region of the sheep MTR gene is not mutated, the PCR amplification product can be completel...

specific Embodiment approach 2

[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the reaction system for PCR amplification in step one is a 10 μL reaction system, which consists of the following components:

[0032]

[0033]

[0034] PCR amplification conditions were: 94°C pre-denaturation for 5 min, 94°C denaturation for 30 s, 65°C annealing for 25 s, 72°C extension for 30 s, a total of 33 cycles, 72°C extension for 7 min, and 4°C incubation. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the enzyme digestion system in step one is as follows:

[0036]

[0037] The enzyme digestion conditions are: 37°C enzyme digestion for 1-2h or overnight. Others are the same as in the first embodiment.

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Abstract

The invention discloses a molecular marking method capable of simultaneously predicting and identifying fineness and crimpness of sheep wool and relates to a molecular marking method. The method comprises the following steps: 1, designing a primer for performing amplification on sheep genome DNA (Deoxyribonucleic acid), and performing enzyme digestion, thereby obtaining an enzyme digestion product; 2, performing electrophoresis separation on the enzyme digestion product, and determining the genotype according to the electrophoresis separation result; 3, performing correlation analysis, and estimating the least squares mean value of the properties, wherein the results are that the wool fineness of the AA, GA genotype group in three genotypes is obviously lower than that of the GG genotype group, and the wool crimpness with the AA genotype group is obviously higher than that of the GG genotype group; and 4, dividing the experimental group into three types, and finishing the operation. The fineness and crimpness of sheep wool of the sheep can be simultaneously predicted and identified, an effective, simple and feasible molecular marking method is provided for quality character improvement and auxiliary mark selection of the sheep wool, and the method can be effectively applied to the field of molecular assisted breeding for superfine wool sheep.

Description

technical field [0001] The invention relates to the field of animal molecular genetics, in particular to a method for simultaneously detecting the fineness and crimp of sheep wool with molecular markers. Background technique [0002] Fine-wool sheep occupy an important position in my country's animal husbandry industry, and the main product of fine-wool sheep is wool. As an important textile raw material, fine wool has high economic value. The production of fine-wool sheep is not only related to the economic development and social stability of the producing areas, but also to the development of my country's wool spinning industry and the balance of import and export trade. With the increasing demand for wool at home and abroad, the cultivation of high-quality fine-wool sheep has become an urgent problem to be solved in the field of wool production and sheep breeding. [0003] The breeding of Chinese Merino sheep (Xinjiang military reclamation type) began in 1972, and has s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王宁荣恩光于磊杨华李辉王守志王志鹏
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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