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Preparation method of tissue engineering scaffold fibrous material

A tissue engineering scaffold and fiber material technology, which can be applied in the fields of fiber chemical characteristics, spinning solution preparation, wet spinning method, etc. Compatibility effect

Inactive Publication Date: 2013-06-19
XIAN REJE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the silk macromolecules in the crystallization region, and the tight spatial structure, general silk products are difficult to be biodegraded, which makes natural silk fibroin in Biomedical applications, especially as tissue engineering materials, are severely limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The pure silk fibroin after natural drying is dissolved in 1.5 liters of 80% phosphoric acid at a temperature of 30°C for 2 hours, filtered and purified, and ultrasonically defoamed for 1 hour to make a spinning stock solution;

[0020] Under the action of 0.4Mpa nitrogen, use a spinning hole with a diameter of 0.5mm, and at a temperature of 25°C, spray out the silk fibroin spinning stock solution at a rate of 2 grams per minute, and enter it with a concentration of 25% ammonium sulfate for solidification. In the liquid, the temperature of the coagulation bath is 30°C, and the primary silk fibroin fibers are coagulated by spinning. The primary silk fibroin fiber washed with water is immersed in the first drawing bath, and the draft ratio is 1 time; then immersed in the second drawing bath, and the draft ratio is 2 times. The drafting solution for the first and second drafting is methanol with a concentration of 60%, and the drafting temperature is 80°C.

[0021] Finall...

Embodiment 2

[0024] The dried pure silk fibroin was dissolved into a silk fibroin mixed solution with 1.5 liters of a ternary solution of lithium bromide, ethanol and water with a weight ratio of 44:50:6 and stirred at a temperature of 75°C for 4 hours. Filtration and purification, ultrasonic defoaming for 1.5 hours to make spinning stock solution.

[0025] Under the action of 03Mpa nitrogen, use 2 spinning holes with a diameter of 0.5mm, and at a temperature of 30°C, spray the silk fibroin spinning stock solution at a speed of 3 grams per minute, and enter the 20°C methanol concentration of 60%. In the coagulation solution, coagulate into primary silk fibroin fibers.

[0026] After washing with water, immerse in the first drawing bath, the drawing solution is 15% ammonium acetate solution, the temperature is 70 ℃, the drawing rate is more than 1.5 times; then immersed in the second drawing bath, the drawing temperature is 80 ℃, the drawing solution It is 10% ammonium acetate solution, an...

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Abstract

The present invention discloses a preparation method of tissue engineering scaffold fibrous material. Silk is used as the main raw material, therefore the obtained regenerated silk fibroin protein fiber has the advantages of good biocompatibility between natural silk fibroin protein and human bodies. Because aggregation structure parameters including crystallinity, orientation degree, etc. of the regenerated silk fibroin protein fiber are controlled in the spinning process, the regenerated silk fibroin protein fiber is biodegradable and becomes an ideal material used for tissue engineering scaffolds.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a preparation method of a tissue engineering scaffold fiber material. Background technique [0002] Tissue engineering is an emerging interdisciplinary field developed in the past 30 years. Its ultimate goal is to produce products for restoring, maintaining, or enhancing the structure and function of human damaged organs or tissues, such as tissue engineered skin, bone, cartilage, tendon etc. Silk is composed of about 75% silk fibroin and 25% sericin. It is a fairly pure natural protein fiber. It is non-toxic and non-irritating to the human body. The amino acid composition of silk fibroin is similar to that of human collagen. With good biocompatibility, it is an ideal raw material for preparing biomedical products. However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F4/02D01F1/00D01D5/06D01D5/14D01D1/02D01C3/02A61L27/22
Inventor 步怀宇
Owner XIAN REJE BIOLOGICAL TECH
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