Tissue engineering fiber material and preparation method thereof

A fiber material and tissue engineering technology, applied in the field of tissue engineering fiber material and its preparation, can solve the problems of difficult biodegradation and limitation, and achieve the effects of good biocompatibility, low cost and good biocompatibility

Inactive Publication Date: 2013-06-19
XIAN REJE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the silk macromolecules in the crystallization region, and the tight spatial structure, general silk products are difficult to be biodegraded, which makes natural silk fibroin in Biomedical applications, especially as tissue engineering materials, are severely limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Put 0.6 kg of leftover raw silk (the by-product of cocoon reeling in the silk reeling factory) into 10 liters of 0.05% sodium carbonate aqueous solution, boil for 0.5 hours, repeat the treatment three times, and remove all the sericin around the silk fibroin to obtain pure silk. Silk fibroin.

[0021] The pure silk fibroin after natural drying is dissolved in 1.5 liters of 80% phosphoric acid at a temperature of 30°C for 2 hours, filtered and purified, and ultrasonically defoamed for 1 hour to make a spinning stock solution;

[0022] Under the action of 0.4Mpa nitrogen, use a spinning hole with a diameter of 0.5mm, and at a temperature of 25°C, spray out the silk fibroin spinning stock solution at a rate of 2 grams per minute, and enter it with a concentration of 25% ammonium sulfate for solidification. In the liquid, the temperature of the coagulation bath is 30°C, and the primary silk fibroin fibers are coagulated by spinning. The primary silk fibroin fiber washed wi...

Embodiment 2

[0025] Put 1 kg of cocoon layer into 20 liters of 0.5% neutral soap solution, boil it for 2 hours, remove the sericin around the silk fibroin, wash it thoroughly with hot water to get pure silk fibroin.

[0026] The dried pure silk fibroin was dissolved into a silk fibroin mixed solution with 1.5 liters of a ternary solution of lithium bromide, ethanol and water with a weight ratio of 44:50:6 and stirred at a temperature of 75°C for 4 hours. Filtration and purification, ultrasonic defoaming for 1.5 hours to make spinning stock solution.

[0027] Under the action of 03Mpa nitrogen, use 2 spinning holes with a diameter of 0.5mm, and at a temperature of 30°C, spray the silk fibroin spinning stock solution at a speed of 3 grams per minute, and enter the 20°C methanol concentration of 60%. In the coagulation solution, coagulate into primary silk fibroin fibers.

[0028] After washing with water, immerse in the first drawing bath, the drawing solution is 15% ammonium acetate soluti...

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Abstract

The present invention discloses a tissue engineering fiber material and a preparation method thereof. The tissue engineering fiber material is prepared by taking silk or silk products as main raw materials. The tissue engineering fiber material retains the protein components of natural silk fibroin, has good biocompatibility and is low in cost. Because of the biodegradability of the tissue engineering fiber material and plasticity of tissue engineering scaffold obtained by postprocessing, the imported high cost biodegradable tissue engineering materials such as polylactic acid can be replaced. The tissue engineering fiber material can be used as tissue engineering scaffold materials or surgical repair materials such as artificial skin, tendon, blood vessel, cartilage, ligament, esophagus, and endocranium.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a tissue engineering fiber material and a preparation method thereof. Background technique [0002] Tissue engineering is a hot spot in life science in the 21st century. Its core content is three-dimensional cell culture in vitro, and its basic method is to form a complex of seed cells and biodegradable biomaterials to replace damaged tissues and organs. Silk is composed of about 75% silk fibroin and 25% sericin. It is a fairly pure natural protein fiber. It is non-toxic and non-irritating to the human body. The amino acid composition of silk fibroin is similar to that of human collagen. With good biocompatibility, it is an ideal raw material for preparing biomedical products. However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the silk macromolecules in the crystallizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F4/02D01D5/06D01D1/02D01D5/14A61L27/22
Inventor 步怀宇
Owner XIAN REJE BIOLOGICAL TECH
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