Fibre for tissue engineering scaffold and making method thereof

A tissue engineering scaffold and fiber technology, applied in the field of biomedical engineering materials, can solve the problems of difficult biodegradation, high price, unfavorable cell attachment, division, proliferation, etc., and achieve the effect of good compatibility

Inactive Publication Date: 2002-10-02
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the silk macromolecules in the crystallization region, and the tight spatial structure, general silk products are difficult to be biodegraded, which makes natural silk fibroin in Biomedical applications, especially as tissue engineering materials, are severely limited
[0003] Before the present invention was made, artificial materials such as polylactic acid were widely used in the world as scaffolds for tissue engineering. Since these materials are prone to local accumulation of acidic substances during the degradation process in the body, it is not conducive to cell attachment, division, Proliferate and expensive
In China, the invention patent "a wound protective film and its preparation method" with the publication number CN1059129C discloses a method of drying and forming a film using silk as a raw material after degumming, dissolving and other processes; the publication number is CN1260363A In the invention patent "A Porous Silk Film and Its Preparation Method", a method for preparing a porous silk film by freeze-drying method is disclosed. For surface covering and artificial skin, it is difficult to form a three-dimensional complex scaffold for tissue engineering
At present, there is no report on the use of natural silk fibroin fibers to prepare biodegradable tissue engineering scaffold materials.

Method used

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  • Fibre for tissue engineering scaffold and making method thereof
  • Fibre for tissue engineering scaffold and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] First put 0.6 kg of leftover raw silk (the by-product of cocoon reeling in the silk reeling factory) into 10 liters of sodium carbonate aqueous solution with a concentration of 0.05%, boil for 0.5 hour, repeat the treatment three times, and remove the sericin on the periphery of the silk to obtain pure silk. Silk fibroin.

[0018] The pure silk fibroin after natural drying is dissolved in 1.5 liters of 80% phosphoric acid at a temperature of 30°C for 2 hours, purified by filtration, and ultrasonically defoamed for 1 hour to make a spinning stock solution;

[0019] See attached figure 1 , under the action of 0.4MPa nitrogen, use a spinning hole with a diameter of 0.5mm, and at a temperature of 25°C, spray the silk fibroin spinning stock solution at a speed of 2 grams per minute, and enter the concentration of 25% ammonium sulfate In the coagulation solution, the temperature of the coagulation bath is 30° C., and the primary silk fibroin fibers are coagulated by spinning...

Embodiment 2

[0022] Put 1 kg of cocoon layer into 20 liters of 0.5% neutral soap solution, boil for 2 hours, remove all the sericin around the silk fibroin, and get pure silk silk fibroin after fully washing with hot water.

[0023] The dried pure silk fibroin was dissolved into a silk fibroin mixed solution with 1.5 liters of a ternary solution of lithium bromide, ethanol and water in a weight ratio of 44:50:6 and stirred at 75°C for 4 hours. Filtration and purification, ultrasonic defoaming for 1.5 hours to make spinning stock solution.

[0024] as attached figure 1 As shown, under the action of 0.3MPa nitrogen, use two spinning holes with a diameter of 0.5mm, and at a temperature of 30°C, spray the silk fibroin spinning stock solution at a speed of 3 grams per minute, and enter the concentration of methanol at 20°C. In the 60% coagulation solution, it is coagulated into primary silk fibroin fibers.

[0025] After washing with water, immerse in the first drawing bath, the drawing solut...

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Abstract

The natural silk can be used as main raw material, and is undergone the processes of degumming, dissolution and purification to obtain silk extract protein solution, then the wet spinning process is adopted and its controlled degree of crystallization is less than 40% and the fibre which can be made into bio-degradable tissue engineering scaffold can be spun. Said fibre possesses good biological compatibility.

Description

technical field [0001] The invention relates to the field of biomedical engineering materials, in particular to a fiber for tissue engineering scaffold and a preparation method thereof. Background technique [0002] Silk is composed of about 75% silk fibroin and 25% sericin. It is a fairly pure natural protein fiber, which is non-toxic and non-irritating to the human body. Its biocompatibility is an ideal raw material for the preparation of biomedical products. However, studies have proved that due to the high crystallinity of natural silk, the strong binding force such as hydrogen bonds inside the silk macromolecules in the crystallization region, and the tight spatial structure, general silk products are difficult to be biodegraded, which makes natural silk fibroin in Applications in the biomedical field, especially as tissue engineering materials, have been severely limited. [0003] Before the present invention was made, artificial materials s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24D01F4/02
Inventor 左葆齐吴徵宇李明忠戴礼兴朱美男
Owner SUZHOU UNIV
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