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Method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum

A Tetrahymena thermophila, transmission electron microscope technology, applied in the preparation of test samples, material analysis using radiation, sampling devices, etc., can solve the problems of other impurity nanoparticles, interference, etc. that cannot be excluded

Inactive Publication Date: 2013-06-19
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for in-situ identification of nano-gold in Tetrahymena thermophila by transmission electron microscopy and energy spectrum, so as to solve the problem that other impurity nanoparticles cannot be excluded in the existing method of observing nanoparticles in organisms with transmission electron microscopy alone. interference problem

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  • Method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum
  • Method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum
  • Method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum

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[0035] 1. Synthesis of gold nanoparticles

[0036] Add 1mL (10 g·L -1 ) of HAuCl 4 , heated to reflux, under vigorous stirring, quickly added 1mL (71.4 g·L -1 ) of trisodium citrate, continue to heat, stir and reflux, and place the prepared gold nanoparticles in a refrigerator at 4°C for later use.

[0037] 2. The cultivation of Tetrahymena and the treatment of nano-gold

[0038] Preparation of liquid medium: yeast powder 2.5 g L -1 , tryptone 2.5 g L -1 , glucose 5 g·L -1 , FeCl 3 Solution 33ml, distilled water 1L, sterilized for later use.

[0039] Inoculate 400uL of Tetrahymena thermophila into an Erlenmeyer flask containing 20ml of liquid medium, and after cultivating in the dark at 30°C for 24 hours, take 400uL from it and re-inoculate it into another Erlenmeyer flask containing 20ml of liquid medium, and add 1.5ml of the step 1 Synthesized gold nanoparticles so that the final concentration of gold nanoparticles in the medium was 7.2 mg L -1 , and cultured at 30°...

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Abstract

The present invention provides a method for identifying nanogold in tetrahymena thermophila in situ by transmission electron microscope and energy spectrum, which is characterized by culturing the tetrahymena thermophila in a gold-containing liquid culture medium for a period of time, cutting into ultrathin sections of 50-70 nm thick with an ultra microtome after the steps of fixation, dehydration, infiltration and embedding, then dredging and drying, dyeing by uranyl acetate and lead citrate, watching under the transmission electron microscope, and confirming by the energy spectrum after watching particles. The invention can eliminate the interference of other impurity nanoparticles, achieve in-situ observation of nanogold particles in tetrahymena thermophila, and provide an effective analysis method for understanding the cycle metabolic process of nano-particles in vivo and evaluating the biotoxicity of the nano-particles.

Description

technical field [0001] The invention belongs to the field of environmental nano particle organism location identification and biological toxicity. Background technique [0002] Nanomaterials and technologies have been widely used, such as antibacterial agents containing nanosilver, fluorescent quantum dot labeling technology widely used in medical imaging, etc., which are closely related to modern life. Nanomaterials and technology is an emerging discipline. In recent years, most nanomaterials have been discharged directly into sewage without treatment, and then into natural water environments such as rivers and oceans. Although the biological effects of nanomaterials and their impact on environmental safety are gradually attracting people's attention, in situ observation of the way nanomaterials enter the organism, the cycle process of nanomaterials in metabolism, and migration and transformation in the food chain The accumulation of organisms has not been further studied....

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Application Information

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IPC IPC(8): G01N23/02G01N1/30G01N1/06
Inventor 徐斌张洪武史俊朋马春艳沈江珊石建稳刘兴强
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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