Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid

A temperature-sensitive chitosan technology, applied in the field of medical biomaterials, can solve problems such as large volume expansion and virus infection

Active Publication Date: 2013-06-26
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, EVICEL and Tisseel are blood products, and there is a possibility of virus infection during use; the safety of BioGlue is still in doubt; and DuraSeal is a synthetic pure chemical material, which is mainly obtained by mixing the two

Method used

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  • Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid
  • Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid
  • Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of thermosensitive chitosan

[0040] In the first step, the chitosan with a degree of deacetylation of 50% is activated with potassium hydroxide with a mass fraction of 70% for 24 hours to become an activated form of hydroxy-K type.

[0041] The second step, hydroxybutylation of chitosan:

[0042] Filter and extrude excess lye in 10 grams of activated hydroxy-K chitosan with 100-mesh silk cloth, transfer to a three-necked bottle, add 100 ml of isopropanol and stir for 1-2 hours to completely disperse chitosan Open, then slowly add 100 ml of 1,2-epoxybutane dropwise with a separatory funnel, react at room temperature for 4-5 days, after the reaction is complete, use 8-10 times the volume of acetone to precipitate, wash, and vacuum dry at 50°C A white powder was obtained.

Embodiment 2

[0043] Embodiment 2: the preparation of thermosensitive chitosan

[0044] In the first step, the chitosan with a deacetylation degree of 30% is activated with potassium hydroxide with a mass fraction of 50% for 36 hours to the activated form of hydroxy-K type.

[0045] All the other steps are the same as in Example 1.

Embodiment 3

[0046] Embodiment 3: the preparation of thermosensitive chitosan

[0047] In the first step, the chitosan with a deacetylation degree of 30% is activated with potassium hydroxide with a mass fraction of 30% for 48 hours to become an activated form of hydroxy-K type.

[0048] All the other steps are the same as in Example 1.

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Abstract

The invention relates to the medical biomaterial technical field. The current product which is widely used for preventing postoperative leakage of cerebrospinal fluid has the disadvantages of tedious operation step, expanded volume and latent infection risk. The invention provides a preparation method for medical grade temperature sensitive hydroxybutyl chitosan capable of internally using, a purifying method and an application method. The prepared material has appropriate deacetylation degree, can guarantee good biocompatibility, and has the advantages of simple operation, complete wound enclosing, injectable performance, biodegradability and excellent plugging effect. According to the invention, a chitosan raw material with deacetylation degree of 10-70% is prepared, then a hydroxyl butylation reaction is carried out, and a unique temperature sensitive performance is endowed for chitosan; and then a unique salting out method and a phase transition method are employed for purifying. The prepared medical grade temperature sensitive chitosan preparation has good temperature sensitive performance, good gel resilience performance, simple usage; and good biocompatibility and biological mechanical property can prove that the preparation method of the medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid has good clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a medical-grade temperature-sensitive chitosan preparation and its preparation method and application. The preparation has good biocompatibility and temperature responsiveness and can be used as a preparation for preventing cerebrospinal fluid leakage to use. Background technique [0002] Chitosan is a naturally occurring ecological alkaline polysaccharide. Years of research have shown that chitosan has very good biological properties and a variety of specific biological functions, such as antibacterial, anti-adhesion, wound healing, etc., because chitosan molecules It contains relatively active 2-NH2 and 6-OH, so a variety of chemical modifications can be carried out to give it more chemical properties and biological functions. For example, the hydroxybutyl chitosan used in this product is chitosan Results of sugar hydroxybutylation. As we all know, the amino grou...

Claims

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Application Information

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IPC IPC(8): C08B37/08A61L15/28A61L15/42
CPCY02P20/141
Inventor 侯春林魏长征周霖奚宏伟蒋丽霞吴剑英
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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