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Sample preparation instrument

A technology of sample preparation and nitrogen blowing plate, which is applied in the field of chemical analysis and pharmaceutical analysis, can solve the problems of poor sample repeatability, large space occupation, and low frequency of use, so as to save laboratory space, be easy to carry and move, and avoid The effect of introducing error

Inactive Publication Date: 2013-06-26
安徽百奥秘科生物医药研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For manual operation, there are the following disadvantages: too much human intervention can easily introduce errors, the repeatability of samples is poor, automation and high throughput cannot be realized, and the organic reagents used in the sample preparation process are relatively harmful to the human body.
At present, 96-well plate or 384-well plate sample pretreatment technology is not used frequently in analytical laboratories or scientific research institutions in my country. The main reason is that there is no matching high-throughput sample preparation instrument; even if there are high-throughput If the sample preparation instrument is used, the nitrogen blowing instrument and oscillator and other instruments required in the subsequent steps are not only expensive but also occupy a relatively large space, which is not conducive to practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1: SPE operation process

[0091] C18 bonded silica filler, 2mL 96-well plate with a specification of 100mg filler / well Operation procedure:

[0092] Placement of sample preparation 96-well C18 packing plate: fix a 2mL 96-well C18 packing plate in the second fixing tank 6 where the upper pretreatment plate of the solid-phase extraction device is placed, so that it completely matches the surrounding. Note that the receiving device below is not put away for the time being, and ensure that the 96-well plate is completely consistent with the surroundings, so as to ensure the sealing of the entire part of the solid-phase extraction.

[0093] Activation: add 2mL of methanol to each well, after adding 1mL for the first time, after equilibrating for 1min, vacuumize to make it flow out, then add 1mL of methanol to activate, and after equilibrating for 1min, vacuumize to make it flow out. Then add 2 mL of water to each well, and activate according to the steps of addi...

Embodiment 2

[0100] Example 2: Medium liquid-liquid extraction (SLE) operation process

[0101] For the operation process of medium-liquid-liquid extraction, the specifications of the 96-well SLE plate used are: 2 mL SLE 96-well plate with a filler content of 200 mg / well.

[0102] Place the receiving plate: place the receiving plate in the groove of the first fixed groove 7 where the receiving plate is placed in the solid phase extraction device.

[0103] Placement of the 96-well SLE plate of the sample preparation plate: Fix the 2mL 96-well SLE plate in the second fixing groove 6 where the upper pretreatment plate of the solid-phase extraction device is placed, so that it completely matches the surrounding.

[0104] Sample loading: pipette 200 μL of 200 μg / mL propylparaben aqueous solution, and load the sample into the SLE column hole. Note: the maximum sample volume should ensure that the sample cannot flow out, and equilibrate for 5 minutes.

[0105] Extraction: pipette 1mL of dichloro...

Embodiment 3

[0108] Example 3: Operation process of novel protein precipitation technology

[0109] In the operation process of rapid protein precipitation technology, the size of the 96-well plate used is 1 mL permeable plate.

[0110] Putting in the receiving plate: placing the receiving plate in the first fixing groove 7 where the receiving plate is placed in the solid phase extraction device.

[0111]Placement of the 96-well PPT plate of the sample preparation plate: fix a 1mL 96-well PPT plate in the second fixing groove 6 of the upper pretreatment plate of the solid-phase extraction device, and ensure that the 96-well plate is completely consistent with the surrounding to ensure that the solid phase Extract the tightness of the entire section.

[0112] Sample loading: pipette 1mL of acetonitrile into the 96-well PPT sample preparation plate with a 96 pipette tip, then pipette 200μL of the sample solution to be treated into the sample well containing 1mL of acetonitrile, shake for 2m...

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Abstract

The invention discloses a sample preparation instrument which comprises an outer shell; a nitrogen blow tank is formed in the top in the shell; the lower part of the nitrogen blow tank is provided with a nitrogen blow plate device; slide ways are respectively arranged at the two sides of the nitrogen blow plate device and in the middle of the nitrogen blow plate device; the nitrogen blow plate device is also provided with nitrogen blow plates capable of sliding along the slide ways; each slide way is provided with a blocking groove, and each nitrogen blow plate is fixed by the corresponding blocking groove; a first fixing groove used for placing a sample liquid receiving plate is formed in the bottom in the outer shell; the first fixing groove is sequentially provided with an intermediate sealing ring and a second fixing groove; the second fixing groove is provided with a sample preparation plate and a solid phase extraction (SPE) bracket; a lever, an oscillator and a negative pressure pump are arranged at the lower part of the first fixing groove; the side surface of the first fixing groove is provided with two small holes which are respectively connected with two fans, and a gas heating device located at the back of the first fixing groove; and the back of the outer shell is provided with a waste liquid outlet hole, an outer nitrogen interface, a negative pressure interface, a power interface and a nitrogen heating device.

Description

technical field [0001] The invention relates to the fields of chemical analysis and pharmaceutical analysis, in particular to a sample preparation instrument. Background technique [0002] In the process of analytical chemistry and pharmaceutical analysis, sample pretreatment is a key link in the accuracy of analysis and detection results. It is an important means to concentrate the measured trace components, improve detection sensitivity and remove interfering substances to the analysis system. The steps of sample pretreatment have a great impact on the high throughput of sample detection, the reliability of data, the cost of analysis, and the satisfaction of experimenters. With the rapid development of sample detection technology, such as: LC-MS (Liquid Chromatography-Mass Spectrum, liquid chromatography and mass spectrometry) technology, GC-MS (Gas Chromatography-Mass Spectrum, gas chromatography and mass spectrometry) technology and CE-MS (Capillary Electrophoresis-Mass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N1/44
Inventor 包建民续竞生马志爽张元津李优鑫
Owner 安徽百奥秘科生物医药研究院有限公司
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