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Cordyceps militaris soybean and preparation method thereof

A technology of Cordyceps militaris and soybeans, which is applied in the field of Cordyceps militaris soybeans and its preparation, and can solve the problems that have not been reported.

Active Publication Date: 2014-03-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are no reports at home and abroad on the biotransformation of soybeans with the Cordyceps militaris fungus, that is, the research on the production of cordycepin in the solid fermentation of soybeans

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) soaking soybeans

[0024] Weighing 250g of soybeans, adding 500g of water, and soaking at room temperature for 12 hours, so that the water content of the soaked soybeans is controlled within the range of 100-120%.

[0025] 2) Sterilization

[0026] After the soaked soybeans are drained, they are divided into Erlenmeyer flasks, covered with stoppers, sterilized at 121°C for 30 minutes, cooled and then inoculated.

[0027] 3) Incline culture of Cordyceps militaris

[0028] Inoculate the preserved Cordyceps militaris strains on the slant medium, and cultivate them for 7-9 days at a temperature of 23°C. The composition of the slant medium is: potato 18-22%, glucose 1-3%, peptone 0.1 ~1.0%, potassium dihydrogen phosphate 0.1~0.5%, magnesium sulfate 0.1~0.3%, vitamin B 1 0.008~ 0.02%, agar 1~3%. One specific formula is as follows: 20% potato, 2% glucose, 0.5% peptone, 0.3% potassium dihydrogen phosphate, 0.25% magnesium sulfate, vitamin B 1 0.01%, agar 2%. The pr...

Embodiment 2

[0049] 1) soaking soybeans

[0050] Weigh 250g of soybeans, add water at a ratio of 1:2 to 1:5, soak at room temperature for 10 to 16 hours, and control the water content of soybeans at 100 to 120%.

[0051] 2) Soybean cellulase treatment

[0052] Drain the soaked soybeans and put them into a triangular flask, add citric acid-disodium hydrogen phosphate buffer solution at a ratio of 1:3 to 5, carry out cellulase treatment at 50°C for 1 to 3 hours, add The amount of enzyme is 8000-12000U / g soybean, rinse with running water for 10-20 minutes, drain, and dry in the shade for 5-8 hours. Cellulase is derived from green wood enzyme with an activity of 11000U / mg.

[0053] 3) Sterilization

[0054] 10-15 mesh peeled soybean granules are divided into Erlenmeyer flasks, covered with stoppers, sterilized at 121°C for 30 minutes, cooled and then inoculated.

[0055] 4) Incline culture of Cordyceps militaris

[0056] Inoculate the preserved Cordyceps militaris strains on the slant med...

Embodiment 3

[0071] 1) soaking soybeans

[0072] Weigh 250g of soybeans, add water at a ratio of 1:2 to 1:5, soak at room temperature for 10 to 16 hours, and control the water content of soybeans at 100 to 120%.

[0073] 2) Soybean cellulase treatment

[0074] Drain the soaked soybeans and put them into a triangular flask, add citric acid-disodium hydrogen phosphate buffer solution at a ratio of 1:3 to 5, carry out cellulase treatment at 50°C for 1 to 3 hours, add The amount of enzyme is 8000-12000U / g soybean, rinse with running water for 10-20 minutes, drain, and dry in the shade for 5-8 hours.

[0075] 3) Matching of two-component solid media

[0076] Mix the cellulase-treated soybeans and peeled peanuts at a ratio of 1:0.1 to 1.0, then add water and mix at a ratio of 2:0.5 to 2.5, and wait for sterilization.

[0077] 4) Sterilization

[0078] The two-component solid medium matched in a certain proportion is covered with a stopper, sterilized at 121°C for 30 minutes, cooled and then ...

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Abstract

The invention relates to a cordyceps militaris soybean. A preparation method comprises the steps of soaking soybeans with water; carrying out sterilization on the soybeans after soaking; after cooling, feeding seed liquid of cordyceps bacteria for fermental cultivation; and then after fermentation, air-drying to obtain the cordyceps militaris soybean. When biological transformation is carried out on cordyceps in the soybeans, the seed coats of the soybeans have proper inhibition effect on the biological transformation. Therefore, after peeling treatment is carried out on the soaked soybeans, the cordycepin content after fermentation can be improved. Moreover, the inoculation area is increased and uniform, and the biological transformation amount of cordyceps cordycepin can be improved. Therefore, through cellulose treatment, the peeling rate of the soaked soybeans is improved; and since the soybeans is subjected to peeling, the transformation amount of cordycepin in the cordyceps militaris soybeans is improved.

Description

technical field [0001] The invention belongs to the technical field of food preparation, and in particular relates to soybeans from Cordyceps militaris and a preparation method thereof. Background technique [0002] Cordyceps militaris (Cordyceps militaris) is a species of Ascomycota, Sclerotinia, Hypocreales, Ergotaceae, Cordyceps genus, and Cordyceps sinensis is the same genus of different fungi, commonly known as militaris. Because it has the same medicinal and nourishing functions as Cordyceps sinensis, and is easier to cultivate artificially than Cordyceps sinensis, Cordyceps militaris is selected as the best substitute of Cordyceps sinensis and a large number of artificial cultivations are carried out, and it has been used as a new resource food and a new class of medicine. Approved by the Ministry of Health and the State Food and Drug Administration to enter the market. [0003] The active components of Cordyceps militaris include cordycepin, adenosine, cordyceps pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/20A23L1/29A23L1/28A23L11/50A23L33/00
Inventor 朴美子王凤舞金玉兰
Owner QINGDAO AGRI UNIV
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