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Micro-fluidic chip for droplet extraction

A microfluidic chip and droplet technology, which is applied in liquid solution solvent extraction, solvent extraction, laboratory containers, etc., can solve the problems of unfavorable liquid-liquid extraction and small specific surface area of ​​droplets, and achieve good extraction effect, Good light transmittance and improved compatibility

Active Publication Date: 2015-01-14
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the droplets produced by this method are plug-type, and the specific surface area of ​​the droplets is small, which is not conducive to liquid-liquid extraction.

Method used

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  • Micro-fluidic chip for droplet extraction
  • Micro-fluidic chip for droplet extraction
  • Micro-fluidic chip for droplet extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, microfluidic chip

[0034] The structure of the present invention is illustrated by taking the direct droplet generation method and the diversion droplet recovery method as examples.

[0035] Such as figure 1 As shown, the microfluidic chip provided by the present invention includes a main channel 6, which is a linear channel, and one end of the main channel 6 is provided with a sample inlet (not marked in the figure), and the sample inlet communicates with the sample introduction tube 1, It is used to inject the sample solution; the other end of the main channel 6 is provided with a waste liquid outlet (not marked in the figure), and the waste liquid outlet is connected with the waste liquid outlet pipe 5 for exporting the waste liquid after extraction; At one end, the main channel 6 communicates with a side channel 9, and the free end of the side channel 9 is an extraction agent inlet (not marked in the figure), and the extraction agent inlet communicat...

Embodiment 2

[0039] Embodiment 2, the hydrophilic modification of microfluidic chip

[0040] Such as image 3 As shown, after the microfluidic chip of the present invention is made, it can be modified by layer-by-layer self-assembly. After water-based, first pass through sodium chloride solution (containing 0.5M sodium chloride) containing 0.1% polyallylamine hydrochloride (PAH) for modification for 10 minutes, rinse with 0.1M sodium chloride solution, pass through containing 0.1 % polystyrene sulfonate (PSS) sodium chloride solution (containing 0.5M sodium chloride) modified for 10min, then washed again with 0.1M sodium chloride solution, and then passed through PAH and The sodium chloride solution of PSS is modified and repeated twice to modify the channel surface of the microfluidic chip to be hydrophilic and maintain it for a long time.

Embodiment 3

[0041] Example 3, the generation of droplets in the microfluidic chip of the present invention-direct droplet generation method

[0042] Such as Figure 4 As shown, the aqueous phase sample solution (simulated by the aqueous solution containing red dye) was passed into the main channel of the microfluidic chip at a flow rate of 5 μL / min, and at the same time, the flow rate of 1 μL / min was used to generate part of the microfluidic chip droplet The extractant solution (simulated by butanol) is passed into the side channel. When the extractant solution enters the main channel, it will be cut by the sample solution flowing in the main channel to form monodisperse droplets, which will flow forward with the sample solution. By adjusting the flow rate of the sample solution and the velocity of the extractant solution, the number and size of the droplets can be adjusted.

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Abstract

The invention discloses a micro-fluidic chip for droplet extraction. The micro-fluidic chip contains a main channel;the main channel is equipped with a sample inlet at one end and a waste outlet at the other end; one end of the main channel near the waste outlet is communicated with a collecting channel; the collecting channel's free end is a sample outlet; near one end of the sample inlet, the main channel communicates with a side channel; the side channel's free end is a extraction agent inlet; there is height difference between the side channel and the main channel; and the side channel's upper edge is located in the bottom of the main channel's lower limb. The micro-fluidic chip provided by the present invention can be used for on-line droplet extraction; when being carried through on-line droplet extraction, the present invention can be adapted to a plurality of continuous flow experimental system; having good compatibility for a plurality of detection methods; enabling real-time on-line determination of substance. The invention can be effectively applied to the biological and medical research of the micro-fluidic chip.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for liquid droplet extraction. Background technique [0002] Microfluidic chip technology has good development prospects in the fields of biology and medicine due to its advantages of low consumption, high throughput, and integration. At present, the microfluidic chip has realized the functions of cell sorting, capture, culture and stimulation, but in order to realize the final application of microfluidic chip technology in the fields of biology and medicine, many microfluidic chip functions need to be further improved and improved. improve. [0003] Studying cell metabolism or drug screening in microfluidic chip is one of the important contents of microfluidic chip technology applied in the field of biology and medicine at the present stage. Documents Gao D.; Wei H.; Guo G.-S.; Lin J.-M.Anal.Chem.,2010,82,5679-568 Cultured human lung epithelial cells A549 in a microfl...

Claims

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Application Information

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IPC IPC(8): B01L3/00B01D11/00B01D11/04G01N1/34
Inventor 林金明王聂君
Owner TSINGHUA UNIV
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