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Extraction method of low-acyl non-clear type gellan gum

An extraction method and gellan gum technology are applied in the field of extraction of low-acyl non-clearing gellan gum, which can solve the problems of not developing a low-acyl non-clearing type gellan gum production process and gel opacity, etc., and achieve excellent dissolution. performance, reduce processing links, and reduce consumption

Inactive Publication Date: 2013-07-17
天茁(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low-acyl non-clear gellan gum products contain some protein (about 17% by mass) and other impurities, so the formed gel is opaque
At present, in the food industry and other fields, the application of low-acyl clear gellan gum is more common, but the application of low-acyl non-clear gellan gum products has not received due understanding, so gellan gum manufacturers currently No specific production process suitable for low-acyl non-clear gellan gum has been developed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The specific process steps of extracting non-clear type low acyl gellan gum in this embodiment are as follows:

[0024] (1) Heating up and pH adjustment of the fermentation broth: directly heat up the gellan gum fermentation broth to 85°C, add potassium hydroxide solution with a mass percentage concentration of 10% to the fermentation broth, and adjust the pH value to 10.0;

[0025] (2) Deacylation treatment of fermentation broth: maintain the fermentation broth obtained in step (1) at 85°C and pH 10.0 for 15 minutes to ensure that the acyl group of gellan gum is completely removed, and deacylated gellan gum is obtained Sol solution;

[0026] (3) Gel and solid-liquid separation of the sol solution: Add sodium chloride accounting for 1.2% of the mass of the sol solution to the gellan gum sol solution obtained in step (2) after deacylation, and add 10% vol. hydrochloric acid solution, adjust the pH value to 4.5, separate the solid and liquid with a chamber filter press a...

Embodiment 2

[0030] The specific process steps of the method for extracting non-clear type low acyl gellan gum in this embodiment are as follows:

[0031] (1) Heating and pH adjustment of the fermentation broth: directly heat the gellan gum fermentation broth to 90°C, add 10% sodium hydroxide solution to the fermentation broth, and adjust the pH value to 10.5;

[0032] (2) Deacylation treatment of fermentation broth: maintain the fermentation broth obtained in step (1) at 90°C and pH 10.5 for 8 minutes to ensure that the acyl group of gellan gum is completely removed, and deacylated gellan gum is obtained Sol solution;

[0033] (3) Gel and solid-liquid separation of the sol solution: add potassium chloride accounting for 1.0% of the mass of the sol solution to the deacylated gellan gum sol solution obtained in step (2), and add potassium chloride with a concentration of 10% by volume Sulfuric acid solution, adjust the pH value to 5.0, and separate the solid and liquid with a chamber filte...

Embodiment 3

[0037] The specific process steps of extracting non-clear type low acyl gellan gum in this embodiment are as follows:

[0038] (1) Heating up and pH adjustment of the fermentation broth: directly heat up the gellan gum fermentation broth to 95°C, add potassium hydroxide solution with a mass percentage concentration of 10% to the fermentation broth, and adjust the pH value to 10.5;

[0039] (2) Deacylation treatment of fermentation broth: maintain the fermentation broth obtained in step (1) at 80°C and pH 10.5 for 8 minutes to ensure that the acyl group of gellan gum is completely removed, and deacylated gellan gum is obtained Sol solution;

[0040] (3) Gel and solid-liquid separation of the sol solution: add potassium chloride accounting for 1.2% of the mass of the sol solution to the deacylated gellan gum sol solution obtained in step (2), and add potassium chloride with a concentration of 10% by volume Sulfuric acid solution, adjust the pH value to 5.5, and separate the sol...

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Abstract

The invention discloses an extraction method of low-acyl non-clear type gellan gum, and the extraction method comprises the following steps of: (1) raising the temperature of fermentation liquor to 70-100 DEG C, alkalizing, and regulating the pH value to 9.0-11.5; (2) keeping for 5-20 minutes, removing acyl to obtain a gellan gum dissolution solution with the acyl removed; (3) adding alkali metal salt which accounts for 0.5-2.0 percent of the weight of the gellan gum dissolution solution to the gellan gum dissolution solution, regulating the pH value to subacidity to form gel, and carrying out solid-liquid separation to obtain a nontransparent gellan gum sheet; (4) cutting the nontransparent gellan gum sheet into gum granules, washing by using lower alcohol, and carrying out solid-liquid separation again to obtain gellan gum powder; and (5) drying and smashing to obtain a low-acyl non-clear type gellan gum finished product. The extraction method disclosed by the invention directly carries out acyl removing treatment on the fermentation liquor by modifying the preprocessing process of the low-acyl gellan gum and omits the filtering and defecating process, thereby decreasing the processing link, reducing the production cost of the gellan gum and ensuring the excellent solubility property of the low-acyl gellan gum product.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for extracting low-acyl non-clear gellan gum. Background technique [0002] Gellan gum (Gellan gum) is a kind of hydrophilic colloid developed by the American Keke (KELCO) company. It is then produced through a series of post-extraction processes. [0003] Gellan gum can be said to be one of the most excellent thickeners and stabilizers at present, and has excellent gel properties. Gellan gum gel is easy to use, has good flavor release, high thermal stability, easy to melt in the mouth, gel time and temperature can be adjusted, the gel is not easily affected by pH, the product is stable, and has various flavors. Texture and other advantages. [0004] At present, gellan gum mainly includes low-acyl clear gellan gum and low-acyl non-clear gellan gum. Due to the filtration process used in the production process of low-acyl clear gellan gum products, most of the ins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 许怀远刘鲁威
Owner 天茁(上海)生物科技有限公司
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