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Quick blended soy sauce judging method

A technology for preparing soy sauce and identification method, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as narrow application, infertility, carcinogenicity, etc., and achieves the effects of high accuracy, high detection efficiency, and simple operation.

Active Publication Date: 2014-08-27
FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two types of identification methods for brewed soy sauce and prepared soy sauce: one is to detect a single characteristic component in the prepared soy sauce for identification. Currently, the index (i.e. characteristic prompt) used to judge brewed soy sauce and non-brewed soy sauce is chloropropanol (3-Monochlo-Propane-1, 2-diol, 3-MCPD) and levulinic acid, but studies have shown that these two characteristic prompts have their limitations: during the production process of AHVPS, the raw material for preparing soy sauce, it is catalyzed by hydrochloric acid, Under high temperature conditions, plant protein can be completely hydrolyzed into free amino acids in about 10 hours, and its side reaction produces 3-MCPD, which can cause cancer and infertility
Zhao Guohua et al. used the difference in the infrared spectra of brewed soy sauce and prepared soy sauce, and combined Fourier transform mid-infrared spectroscopy with the least partial squares method to achieve category analysis and identification of brewed soy sauce, prepared soy sauce, and AHVPS. However, because this method is Use the characteristic fingerprint peaks of brewed soy sauce in the infrared spectrum for identification, but the infrared characteristic fingerprint peaks of brewed soy sauce of different brands and different brewing processes are not completely similar, so the method is applicable to a narrow range

Method used

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Experimental program
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Effect test

Embodiment 1

[0026] The fast identification method of preparing soy sauce in the present embodiment is:

[0027] (1) Take 1mL of the soy sauce sample to be tested, and dilute to 20mL with first-grade water as the test solution.

[0028] (2) Accurately weigh valyl-leucine (Val-Leu), valyl-isoleucine (Val-Ile), leucyl-valine (Leu-Val), isoleucyl - Valine (Ile-Val) standard reference substance 20mg each, dissolved in first-grade water, dilute to 10mL, shake well, and prepare mixed standard solution.

[0029] (3) Use HPLC-MS to detect the above-mentioned test solution and mixed standard solution respectively.

[0030] The HPLC conditions are as follows: the chromatographic column is a C18 column, 250×4.6mm, the column temperature is 40°C, the flow rate is 1mL / min, the UV detection wavelength is 220nm, and the elution mobile phase ratio is water-acetonitrile (85:15, v / v ), and the injection volume was 25 μL.

[0031]The MS conditions are set as follows: the ion source is ESI, the scanning ra...

Embodiment 2

[0036] The fast identification method of preparing soy sauce in the present embodiment is:

[0037] (1) Take 1mL of the soy sauce sample to be tested, add 1mL of 0.1mol / L borate buffer solution (pH10.4), 0.5mL of 10mg / mL o-phthalaldehyde and 0.5mL of 10mg / mL β-mercaptoethanol mL, dilute to 20mL with first-grade water, shake well, and use it as the test solution.

[0038] (2) Accurately weigh 20 mg each of Val-Leu, Val-Ile, Leu-Val, and Ile-Val standard reference substances, dissolve them in first-grade water and dilute to 10 mL, shake well, take 1 mL of the solution, and add 0.1 mol / L borate buffer solution (pH10.4) 1mL, 10mg / mL o-phthalaldehyde 0.5mL and 10mg / mL β-mercaptoethanol 0.5mL, dilute to 10mL with primary water, shake well, as Mix the standard solutions.

[0039] (3) Use HPLC-MS to detect the above-mentioned test solution and mixed standard solution respectively.

[0040] The HPLC conditions are as follows: the chromatographic column is a C18 column, 150×4.6mm, t...

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Abstract

The invention discloses a quick blended soy sauce judging method. The quick blended soy sauce judging method includes: detecting whether special substances exist in soy sauce samples to be detected or not, if yes, judging the soy sauce samples to be blended soy sauce, wherein the special substances can be any one or more kinds of valyl-leucine, valyl-leucine derivatives, valyl-isoleucine, valyl-isoleucine derivatives, leucyl-valyl, leucyl-valyl derivatives, isoleucyl-valyl, and isoleucyl-valyl derivatives. The quick blended soy sauce judging method is wide in application range, high in accuracy and simplicity in operation.

Description

technical field [0001] The invention relates to an identification method for soy sauce, in particular to a rapid identification method for preparing soy sauce. Background technique [0002] Soy sauce is a traditional brewing product in my country. It has a history of more than 2,000 years and has become an indispensable condiment in the daily life of ordinary people. At present, the annual output of soy sauce in my country reaches 5 million tons, accounting for 60% of the world's annual output, and the annual output value exceeds 16 billion yuan. According to different production processes, soy sauce is divided into two types: brewed soy sauce and prepared soy sauce. Among them, brewed soy sauce refers to a liquid condiment with special color, aroma and taste made from soybean, wheat or bran through microbial fermentation; and brewed soy sauce is mainly made of brewed soy sauce, mixed with acid hydrolyzed vegetable protein liquid (AHVPS) and food additives are mixed and pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 童星谭丽贤李术丹李贤信黄文彪刘向铧
Owner FOSHAN HAITIAN FLAVOURING & FOOD CO LTD