Quick blended soy sauce judging method
A technology for preparing soy sauce and identification method, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as narrow application, infertility, carcinogenicity, etc., and achieves the effects of high accuracy, high detection efficiency, and simple operation.
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Embodiment 1
[0026] The fast identification method of preparing soy sauce in the present embodiment is:
[0027] (1) Take 1mL of the soy sauce sample to be tested, and dilute to 20mL with first-grade water as the test solution.
[0028] (2) Accurately weigh valyl-leucine (Val-Leu), valyl-isoleucine (Val-Ile), leucyl-valine (Leu-Val), isoleucyl - Valine (Ile-Val) standard reference substance 20mg each, dissolved in first-grade water, dilute to 10mL, shake well, and prepare mixed standard solution.
[0029] (3) Use HPLC-MS to detect the above-mentioned test solution and mixed standard solution respectively.
[0030] The HPLC conditions are as follows: the chromatographic column is a C18 column, 250×4.6mm, the column temperature is 40°C, the flow rate is 1mL / min, the UV detection wavelength is 220nm, and the elution mobile phase ratio is water-acetonitrile (85:15, v / v ), and the injection volume was 25 μL.
[0031]The MS conditions are set as follows: the ion source is ESI, the scanning ra...
Embodiment 2
[0036] The fast identification method of preparing soy sauce in the present embodiment is:
[0037] (1) Take 1mL of the soy sauce sample to be tested, add 1mL of 0.1mol / L borate buffer solution (pH10.4), 0.5mL of 10mg / mL o-phthalaldehyde and 0.5mL of 10mg / mL β-mercaptoethanol mL, dilute to 20mL with first-grade water, shake well, and use it as the test solution.
[0038] (2) Accurately weigh 20 mg each of Val-Leu, Val-Ile, Leu-Val, and Ile-Val standard reference substances, dissolve them in first-grade water and dilute to 10 mL, shake well, take 1 mL of the solution, and add 0.1 mol / L borate buffer solution (pH10.4) 1mL, 10mg / mL o-phthalaldehyde 0.5mL and 10mg / mL β-mercaptoethanol 0.5mL, dilute to 10mL with primary water, shake well, as Mix the standard solutions.
[0039] (3) Use HPLC-MS to detect the above-mentioned test solution and mixed standard solution respectively.
[0040] The HPLC conditions are as follows: the chromatographic column is a C18 column, 150×4.6mm, t...
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