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Preparation method of pig spleen transfer factor injection

A technology of pig spleen transfer factor and injection, which is applied in the field of preparation of biological products, can solve the problems of low utilization rate of active ingredients, long production cycle, and destruction of biologically active ingredients, and achieve the effects of shortening the production cycle, low cost, and easy material acquisition

Active Publication Date: 2013-07-24
SHANDONG SINDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of pig spleen transfer factor injection, to solve the problems of the destruction of the bioactive components of the above-mentioned process, low utilization rate of active components and long production cycle

Method used

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  • Preparation method of pig spleen transfer factor injection
  • Preparation method of pig spleen transfer factor injection
  • Preparation method of pig spleen transfer factor injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation method of porcine spleen transfer factor injection

[0031] A preparation method of porcine spleen transfer factor injection, the specific steps are as follows:

[0032] S1: Collection and inspection of pig spleen;

[0033] Raw materials are selected from standardized pig breeding and slaughtering enterprises to collect pig spleen, standardized pig breeding and slaughtering enterprises, this slaughtering enterprise should have the "Animal Epidemic Prevention Certificate", and the slaughterhouse should comply with the national standard of the People's Republic of China GB / T17236-1998 "Operational Specifications for Pig Slaughter" "Stipulates that the hygienic requirements should comply with the provisions of the "Hygienic Standards for Meat Processing" GB12649. The raw material used in this example—pig spleen comes from Shandong Delisi Group, which meets the raw material location and slaughter requirements of pig spleen.

[0034] Pig splee...

Embodiment 2

[0045] Embodiment 2: the preparation method of porcine spleen transfer factor injection

[0046] A preparation method of porcine spleen transfer factor injection, the specific steps are as follows:

[0047] S1: Collection and inspection of pig spleen;

[0048] Raw materials are selected from standardized pig breeding and slaughtering enterprises to collect pig spleen, standardized pig breeding and slaughtering enterprises, this slaughtering enterprise should have the "Animal Epidemic Prevention Certificate", and the slaughterhouse should comply with the national standard of the People's Republic of China GB / T17236-1998 "Operational Specifications for Pig Slaughter" "Stipulates that the hygienic requirements should comply with the provisions of the "Hygienic Standards for Meat Processing" GB12649. The raw material used in this example—pig spleen comes from Shandong Delisi Group, which meets the raw material location and slaughter requirements of pig spleen.

[0049] Pig splee...

Embodiment 3

[0060] Embodiment 3: the preparation method of porcine spleen transfer factor injection

[0061] A preparation method of porcine spleen transfer factor injection, the specific steps are as follows:

[0062] S1: Collection and inspection of pig spleen;

[0063] Raw materials are selected from standardized pig breeding and slaughtering enterprises to collect pig spleen, standardized pig breeding and slaughtering enterprises, this slaughtering enterprise should have the "Animal Epidemic Prevention Certificate", and the slaughterhouse should comply with the national standard of the People's Republic of China GB / T17236-1998 "Operational Specifications for Pig Slaughter" "Stipulates that the hygienic requirements should comply with the provisions of the "Hygienic Standards for Meat Processing" GB12649. The raw material used in this example—pig spleen comes from Shandong Delisi Group, which meets the raw material location and slaughter requirements of pig spleen.

[0064] Pig splee...

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Abstract

The invention relates to a preparation method of pig spleen transfer factor injection. The preparation method comprises the following steps: performing pretreatment on a pig spleen which is inspected to be qualified, and then preliminarily mincing once to three times; finely mincing and grinding the pig spleen after preliminary mincing for 1.5-8 minutes to prepare a homogenate; adding a formaldehyde solution into the homogenate for inactivation; repeatedly freezing and thawing the inactivated homogenate 6-10 times; performing centrifugal separation, filtration and ultra-filtration on the homogenate which is repeatedly frozen and thawed to get a semi-finished product; and adding water for injection into the semi-finished product which is inspected to be qualified to get a finished product. The preparation method disclosed by the invention has the advantages of simple process and low cost, and can greatly shorten production cycle to 4 days, greatly save manpower and material resources and improve the production efficiency of a transfer factor.

Description

technical field [0001] The invention relates to a preparation method of biological products, in particular to a preparation method of porcine spleen transfer factor injection. Background technique [0002] In the modern intensive breeding industry, there are many kinds of frequent and multiple infectious diseases, and the application of vaccination to prevent various infectious diseases has become the key to the success of the breeding industry. The immune effect of vaccines is affected by various external and internal factors. External factors include vaccine quality, storage, and use methods. There are many factors that affect the functional state of the body's immune system, such as certain diseases, stress, application of antibiotics, malnutrition, etc., can reduce or inhibit the body's immune system function and affect the immune effect. The development and application of animal immune enhancers to improve the body's specific and non-specific immune system functions, i...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61K35/28A61K9/08A61P37/04A61K35/26
Inventor 李朝阳乔彦良
Owner SHANDONG SINDER TECH
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