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Micro-fluid control device capable of simultaneously exerting mechanical stimulation and chemical stimulation

A technology of microfluidic chips and microfluidic channels, which can be used in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., and can solve problems that do not involve chemical stimuli

Active Publication Date: 2013-07-24
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent applications (Application Nos. 201110404504.6 and 201110404620.8) relate to a microfluidic device for simulating arterial vessels and its application, mainly realizing long-term mechanical stimulation of cells on a microfluidic chip, but not involving the introduction of chemical stimuli chip, thereby simulating diseases such as atherosclerosis, a disease caused by chemical and mechanical stimuli acting together on vascular endothelial cells

Method used

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  • Micro-fluid control device capable of simultaneously exerting mechanical stimulation and chemical stimulation
  • Micro-fluid control device capable of simultaneously exerting mechanical stimulation and chemical stimulation
  • Micro-fluid control device capable of simultaneously exerting mechanical stimulation and chemical stimulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of microfluidic chip

[0044] The polymethyl methacrylate template was prepared by mechanical processing. After the surface was treated with perfluorosilane, the polydimethylsiloxane template was prepared by soft etching technology and the polydimethylsiloxane module was prepared by the second mold, including Microfluidic Channel Module ( figure 1 ) and the negative pressure generating module ( figure 2 ). Among them, the pipe D of the microfluidic channel module is 20mm long, 1mm wide, and 0.4mm high; three cylindrical holes A, B, and C run through the entire module, about 2mm high, and 0.8mm in diameter. The total length of the microfluidic channel module is about 30mm, and the width is about 15mm. The total length of the negative pressure generating module is about 30mm, the width is about 15mm, and the thickness is about 2mm. The groove on the negative pressure generating module is 1mm deep. Other specific data such as image 3 shown. The distanc...

Embodiment approach

[0048] The embodiment of utilizing the model of the present invention to reproduce the normal microenvironment of vascular endothelial cells in vivo is as follows:

[0049] 1. Fabricate a microfluidic chip according to the method in Example 1.

[0050] 2. Inoculate vascular endothelial cells into the microfluidic channel through fluid outlet B and inlet C on the microfluidic channel module, and culture them for 12 hours after they attach to the wall.

[0051] 3. Use a PE hose with an outer diameter of 0.8mm to connect the fluid inlet and outlet B and C to form a loop. The loop is filled with medium, and the loop is connected to a peristaltic pump. The flow rate of the circulating liquid flow is controlled by the peristaltic pump. The fluid shear stress suffered by the cells is controlled between 1.16Pa and 5.07Pa (the range of normal fluid shear stress suffered by cells in vivo).

[0052] 4. Use a PE hose with an outer diameter of 0.8mm to connect the through hole A with the ...

Embodiment 3

[0056] Using the model of the present invention to reproduce the microenvironment of vascular endothelial cells in vivo, changing the chemical stimulation conditions in the culture medium alone, and providing a hyperglycemic environment for vascular endothelial cells, one of the embodiments is as follows:

[0057] 1. Fabricate a microfluidic chip according to the method in Example 1.

[0058] 2. Inoculate vascular endothelial cells into the microfluidic channel through fluid outlet B and inlet C on the microfluidic channel module, and culture them for 12 hours after they attach to the wall.

[0059] 3. Use a PE hose with an outer diameter of 0.8mm to connect the fluid inlets B and C to form a loop, add 20mmol / L glucose to the culture medium and fill the loop, and connect the loop to a peristaltic pump, which is controlled by the peristaltic pump The flow rate of the circulating liquid flow controls the fluid shear force suffered by the cells within 1.16Pa to 5.07Pa (the range ...

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Abstract

The invention discloses a micro-fluid control device capable of simultaneously exerting mechanical stimulation and chemical stimulation. The micro-fluid control device is applied to in-vitro simulation of atherosclerosis and comprises a micro-fluid control chip, a cell culture driving system and a negative pressure generator, wherein the cell culture driving system is connected with the micro-fluid control chip so as to drive liquid in a micro-fluid control channel of the micro-fluid control chip to flow; and the negative pressure generator is connected with the micro-fluid control chip so as to generate a negative pressure. By utilizing the device, the influence of the mechanical stimulation and chemical stimulation on vascular endothelial cells in blood vessels can be simulated, so as to research the occurrence and the development of the atherosclerosis and further carry out drug screening and exploration of therapeutic methods.

Description

technical field [0001] The invention relates to a microfluidic chip capable of simultaneously applying mechanical stimulation and chemical stimulation and a microfluidic device with the chip, specifically for simulating atherosclerosis in vitro. Background technique [0002] Existing microfluidic technology is the technology of controlling gas and fluid in micropipes. This technique is simple to operate and can be easily combined with various commonly used biochemical analysis methods, so it is widely used in biochemical analysis. Atherosclerosis is the most common and harmful disease. It is a type of arteriosclerosis. Yellow substances containing cholesterol and fat-like substances appear in the intima of large and medium arteries. Disorder caused. Often lead to thrombosis, blood supply disorders. Atherosclerosis is more common in men over the age of 40 and in postmenopausal women. The disease is often accompanied by hypertension, hypercholesterolemia or diabetes. This...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 蒋兴宇姜博郑文富张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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