Chemiluminescent immunoassay method for 3,5,3'-triiodothyronine in blood serum

Abstract

The invention relates to a chemiluminescent immunoassay method for 3,5,3'-triiodothyronine in blood serum. The method comprises the steps of preparing T3 standard substance, preparing and diluting enzyme marker, coating envelope antigen into a precoated reaction plate, preparing luminescent substrate solution, preparing washing concentrate and the like. According to the chemiluminescent immunoassay method for 3,5,3'-triiodothyronine in blood serum, the detection sensitivity and detection range can be greatly detected by combining chemiluminescent technology on the basis of enzyme immunoassay, the limitness of the traditional chemiluminescent substrate solution can also be changed, the manner that two luminescent substrate solutions are mixed to be used is changed, only one luminescent substrate solution is used, so that the operation is simpler, thereby bringing greater convenience and flexibility for large-scale production and clinical application of products.

Description

technical field [0001] The invention relates to a chemiluminescence immunoassay method for 3,5,3'-triiodothyronine in serum, belonging to the technical field of clinical blood immunoassay methods. Background technique [0002] 3,5,3'-Triiodothyronine (T3) is an important thyroid hormone secreted by the thyroid gland. Except a small part of T3 in blood circulation is directly released by thyroid tissue, most of it is converted from thyroxine (T4) deiodination. Both T3 and T4 are regulated by the hypothalamus-pituitary-thyroid axis, and cooperate to maintain thyroid function, and play an important role in regulating the growth, development, metabolism and systemic hormone balance of the body. The total amount of T3 in serum is only 1 / 60 of that of T4, but its biological activity is 5 times greater than that of T4. [0003] T3 is one of the most sensitive indicators for diagnosing hyperthyroidism. It can specifically reflect the functional state of the thyroid gland and has h...

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Problems solved by technology

However, these two methods have their disadvantages, such as complex operation of radioimmunoassay, long time, and radioactive contamination, while enzyme immunoassay has low sensitivity and narrow detection range; the sandwich method has high cost, poor stability, and At present, there is no paired antibody at home and abroad, so the sandwich method cannot be used for detection; and the chemiluminescence method can improve the sensitivity of detection, but because the traditional chemiluminescence substrate solution is composed of A solution (oxidant) and B solution (luminescent agent) Partial composition, liquid A and liquid B are stored separately, when using, mix the same volume of liquid A and liquid B, and use it up within 10 minutes, otherwise its stability will deteriorate, so it will also cause a sharp drop in the test signal value Decrease, the detection range is obviously narrowed, the stability is worse, and it is not convenient for clinical application