Liquid-state calibration product for determining ProGRP content

Abstract

The invention discloses a liquid-state calibration product for determining the ProGRP content. The liquid-state calibration product for determining the ProGRP content is prepared from the following components: 10 to 200mmol / L of buffer solution, 0.1 to 0.5 percent (w / v) of preservative, 1 to 5 percent (w / v) of protein stabilizer, 5 to 10 percent (w / v) of sugar stabilizer, 1 to 5 percent (w / v) of EDTA (Ethylene Diamine Tetraacetic Acid), 0.1 to 0.5 percent (w / v) of surfactant, 0 to 5000pg / mL of ProGRP antigen, and 1 to 10mg / L of aprotinin. The liquid-state calibration product for determining the ProGRP content provided by the invention has the advantages that no freeze drying is needed, no redissolving is needed when in use, uncontrollable factors probably occurred in a redissolving processare reduced, and a clinical detection result is more accurate. The sugar stabilizer, a metalloproteinase inhibitor (EDTA), the aprotinin and other components are added, so that the stability of the ProGRP protein is effectively improved, and the prepared ProGRP liquid-state calibration product is better in stability and can be stably stored for 12 months by being refrigerated at 2 to 8 DEG C.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a liquid calibrator used for measuring the content of gastrin releasing peptide precursor (ProGRP). Background technique [0002] Gastrin-releasing peptide (GRP) is a gastrin-stimulating gastrointestinal hormone isolated from pig gastric tissue cells by McDonald et al. in 1978. It consists of 27 amino acids and is an effective prophylaxis Secretins or regulatory peptides are widely distributed in the gastrointestinal nervous system and pulmonary airways of mammals. Lung cancer cancer cells can also produce and secrete GRP to regulate their own growth in an autocrine manner, and can also bind to corresponding receptors on the surrounding tumor cell membrane through local diffusion to stimulate tumor growth. Low levels of GRP can stimulate DNA synthesis in small cell lung cancer (SCLC) cells, so GRP is considered to be an autonomous growth factor of SCLC. However, because GRP is ea...

AI Technical Summary

Problems solved by technology

At present, the ProGRP calibrators provided by well-known manufacturers of in vitro diagnostic reagents at home and abroad (such as Roche, Kang Neger, etc.) are mainly freeze-dried products, which are not only expensive, but also have the following disadvantages: a. b. During the reconstitution process, the difference in water quality and human error may cause the concentration of the reconstituted calibrator to deviate from the target value, resulting in inaccurate clinical test results; c. Reconstitution of the calibrator It is not easy to store afterward, and the storage time is short

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