Preparation method of antibiotic medicine erythromycin high-efficiency degradation microbial inoculum

A technology for efficiently degrading bacteria and erythromycin, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsatisfactory treatment effect of erythromycin, high energy consumption, high investment, etc., and achieve accelerated successful start-up , Efficient degradation, high safety effect

Inactive Publication Date: 2013-07-31
DONGHUA UNIV +1
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  • Abstract
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  • Claims
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Problems solved by technology

Although the membrane filtration method can remove erythromycin in water more efficiently, it has the disadvantages of high energy consumption and high investment. In addition, the treatment of the c

Method used

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  • Preparation method of antibiotic medicine erythromycin high-efficiency degradation microbial inoculum
  • Preparation method of antibiotic medicine erythromycin high-efficiency degradation microbial inoculum
  • Preparation method of antibiotic medicine erythromycin high-efficiency degradation microbial inoculum

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Embodiment 1

[0026] 1. Isolation and identification of strains

[0027] (1) Put the activated sludge into the culture medium at an inoculum amount of 10%, and acclimatize with the gradient pressure acclimation method. Under the conditions of culture temperature of 30°C and stirring speed of 160r / min, the filling volume of 250mL Erlenmeyer flask was 100mL for acclimation culture, and the total acclimatization time was about 3 months. The formula of inorganic salt medium is: K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 C12.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O0.01g, CaCl 2 2H 2 O0.03g, distilled water 1000mL, pH=6.8~7.0.

[0028] (2) Take 1mL of the above acclimatized culture solution, and use sterilized physiological saline to make 10 -1 ~10 -6 Gradually dilute, spread evenly on the solid medium with a coating rod, and then place the medium in a light incubator at 30°C for 48 hours. Single colonies with clear morphology were picked and numbered, and then purified repeatedly. ...

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Abstract

The invention relates to a preparation method of an antibiotic medicine erythromycin high-efficiency degradation microbial inoculum. The method comprises the steps that: (1) active sludge is inoculated into a culture medium, and is acclimated with a gradient pressure acclimation method; after acclimation, the material is settled; upper-layer water sample is fetched; separation is carried out by using a coating separation method and a plate streaking method after gradient dilution; culturing and purification are carried out; the separated strain is inoculated into beef extract and peptone culture media, and is subjected to shaking culturing until a logarithmic growth phase is reached; and (2) the obtained strain is inoculated into a culture medium and is cultured, such that the microbial inoculum is obtained. The preparation method provided by the invention is simple and feasible, and has the advantages of high safety and low cost. The obtained erythromycin degradation microbial inoculum is convenient to use, and can degrade the antibiotic medicine erythromycin highly efficiently. The strain can easily be deactivated, and can be used in erythromycin effective removing in water treatments.

Description

technical field [0001] The invention belongs to the field of preparation of degrading bacterial agents, in particular to a preparation method of antibiotic drug erythromycin with high efficiency degrading bacterial agents. Background technique [0002] With the rapid development and application of new environmental analysis tools, as well as the continuous enhancement of people's environmental awareness, the focus of environmental workers in the field of environmental pollution and human health has gradually extended from traditional typical pollutants to the presence in the environment. Emerging trace pollutants, such as antibiotic drugs, etc. In recent years, various concentrations of antibiotics have been detected in sewage, surface water, groundwater, and even drinking water. Studies have shown that long-term exposure to environmental concentrations of antibiotics can cause serious harm to organisms such as chronic toxicity, endocrine disruption, and evolution of drug r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/40
Inventor 高品黄圣琳何梦琦李侃竹王宇晖薛罡刘亚男刘振鸿张慧芬刘畅吴立乐刘路石为民
Owner DONGHUA UNIV
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